: Antioxidant of tomato (Lycopersicon esculentum Mill. cv MT1) that have been pla nted according to split split plot experimental design and subjected to sixteen (n=16) treatment s namely as T1 to T16 were evaluated. The antioxidant of tomato extracts were determined by three methods namely total phenolic content (TPC), free radical scavenging activity (DPPH) a nd Ferric Reducing Antioxidant Power (FRAP). The highest mean efficiency for TPC and DPP H values in tomato were from T7, 1163.6 mg Gallic acid equivalent (GAE)/100 g and 55.7 % ( chemical pesticide, mixture of organic and chemical fertilizer; and growth medium in cow ma nure compost). However T3, 54.2 % (chemical pesticide, organic fertilizer and growth mediu m in cow manure compost) showed significantly highest for DPPH only. FRAP values for T2, 9.00 μmol trolox equivalent (TE)/100 g (chemical pesticide, without fertilizer and growth med ium in EFB compost) treatment showed significantly higher (p<0.05) than other treatment. Pea rson coefficient correlation test showed positive correlation (p<0.05) between TPC and DPPH assay (r=0.933) and FRAP assay (r=0.874), respectively showed that the phenolic compounds was a contributor of the antioxidant activity in tomato. Thus, the finding of this study demonst rated that pesticide, fertilizer and growth medium in compost factor and their interaction did n ot show any specific patterns content toward TPC, DPPH and FRAP; while the TPC was the m ain contributor of antioxidant activity in tomat