Phenolic compounds in leaves of<i>Salix</i>species and hybrids growing under different soil conditions

Gąsecka, Monika; Mleczek, Mirosław; Jutrzenka, Agata; Goliński, Piotr; Stuper‐Szablewska, Kinga

doi:10.1080/02757540.2017.1289186

Cited by 23 publications

(11 citation statements)

References 43 publications

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“…Their presence has been reported in the leaves of trees such as Betula pendula [14], Salix sp. [15] and Aesculus sp. [16,17] as well as the leaf litter of Central Europe's forests [18].…”

Section: Introductionmentioning

confidence: 99%

Laccase activity of the ascomycete fungus Nectriella pironii and innovative strategies for its production on leaf litter of an urban park

et al. 2020

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A laccase-producing ascomycete fungus was isolated from soil collected around the premises of a textile dye factory and identified as Nectriella pironii. Efficient laccase production was achieved via the synergistic action of 1 mM copper sulfate and ferulic acid. Extracts of rapeseed oil cake, grass hay, and leaf litter collected in a pocket urban park were used for enzyme production. The highest laccase activity (3,330 U/L) was observed in the culture grown on the leaf litter extract. This is the first report on biosynthesis of laccase by N. pironii. This is also the first study on utilization of naturally fallen park leaves as a substrate for fungal laccase production. The extracellular enzyme possessing laccase activity was purified to homogeneity by ion-exchange and gel filtration chromatographic techniques. The amino acid sequence of the protein revealed highest similarity to the laccase enzyme produced by Stachybotrys chartarum-and considerable homology to those produced by other fungal species. The purified laccase possessed a molecular mass of 50 kDa. The enzyme had an optimum pH of 2.0 or 6.0 and retained more than 50% of residual activity after 3 hours of incubation at pH 3.0-10.6 or 4.0-9.0 when 2,2 0-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid or 2,6-dimethoxyphenol, respectively, were used. Dithiothreitol, β-mercaptoethanol, and sodium azide at 1 mM concentration strongly inhibited the laccase activity, while in the presence of 50 mM urea, the enzyme was found to retain 25% of its activity. The laccase was able to decolorize more than 80% of Indigo Carmine, Remazol Brilliant Blue R, Reactive Orange 16, and Acid Red 27 dyes within 1 h. The possibility of leaf litter use for the production of the laccase enzyme from N. pironii (IM 6443), exhibiting high pH stability and degradative potential, makes it a promising tool for use in different environmental and industrial operations.

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“…Their presence has been reported in the leaves of trees such as Betula pendula [14], Salix sp. [15] and Aesculus sp. [16,17] as well as the leaf litter of Central Europe's forests [18].…”

Section: Introductionmentioning

confidence: 99%

Laccase activity of the ascomycete fungus Nectriella pironii and innovative strategies for its production on leaf litter of an urban park

et al. 2020

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“…Similar concentrations of polyphenols were obtained after applying a water/ethanol mixture (30/70, v/v ) for maceration or ultrasound-assisted extraction or accelerated solvent extraction. In leaves of Salix species or hybrids, polyphenol concentration after extraction with methanol and HCl (99/1, v/v ) ranged from 18.19 to 84.71 mg GAE/g d.m [ 52 ]. These values were similar to those obtained in this study after maceration and extraction with scCO 2 and were much lower compared to those following extraction with scCO 2 and water ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Activity and Chemical Characteristics of Supercritical CO2 and Water Extracts from Willow and Poplar

et al. 2021

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Renewable feedstock from perennial industrial crops, including those cultivated on marginal land in a short-rotation coppice system, could be an important contribution to the bioeconomy. The majority of data available on the topic are limited to the production of bioenergy from this type of biomass. According to the concept of bioeconomy, biomass-based bioproducts have priority over energy production. This paper characterizes the chemical composition and antioxidant activity of extracts from bark (b), wood (w) or a mixture of bark and wood (b + w) from Salix purpurea, Salix viminalis and Populus nigra obtained using supercritical carbon dioxide (scCO2), scCO2 and water (1%, w/w) or only water. Generally, a high concentration of polyphenols was obtained after extraction with scCO2 and water, while the lowest concentration was found in extracts obtained with scCO2. The highest concentration of polyphenols (p < 0.05) was obtained in an extract from P. nigra (b) (502.62 ± 9.86 mg GAE/g dry matter (d.m.)) after extraction with scCO2 and water, whereas the lowest polyphenol concentration was observed in an scCO2 extract from S. purpurea (b) (6.02 ± 0.13 mg GAE/g d.m.). The flavonoids were effectively separated by extraction with scCO2 (0.88–18.37 mg QE/g d.m.). A positive linear relationship between the antioxidant activity determined by DPPH and ABTS assays and the concentration of polyphenols was demonstrated, R2 = 0.8377 and R2 = 0.9568, respectively. It is most probable that the concentration of flavonoids, rather than the concentration of polyphenols, determines the chelating activity of Fe2+. The Fe2+-chelating activity of scCO2 extracts ranged from 75.11% (EC50 = 5.41 mg/cm3, S. purpurea, b + w) to 99.43% (EC50 = 0.85 mg/cm3, P. nigra, b + w). The lowest chelating activity was demonstrated by the extracts obtained with scCO2 and water (maximum 26.36%, S. purpurea, b + w). In extracts obtained with scCO2 and water, p-hydroxybenzoic acid (210–428 µg/g), p-coumaric acid (56–281 µg/g), saligenin (142–300 µg/g) and salicortin (16–164 µg/g) were the dominant polyphenols. All of these chemical compounds occurred mainly in the free form. The S. purpurea, S. viminalis and P. nigra biomass proved to be an attractive source of biologically active compounds for various possible applications in food, drugs or cosmetics. These compounds could be extracted using an environmentally friendly method with scCO2 and water as a co-solvent.

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“…Gąsecka group reported that the accumulation of phenolic compounds in Salix spp. leaves were higher than plants grown in areas with high concentrations of Cu, Pb, and Zn elements (Gąsecka et al, 2017). Cu in the form of Cu(NO 3 ) 2 was also reported to increase the biosynthesis of growth regulators and phenolic content in Salix purpurea × viminalis, reported by Drzewiecka et al, 2017(Drzewiecka et al, 2017.…”

Section: Introductionmentioning

confidence: 85%

Evaluating the Content of Some Metal Elements in Soil and Their Effects on the Total Phenolic and Flavonoid Contents of Some Medicinal Plants Using X-Ray Fluorescence (XRF) Method

Nguyen

Huynh

Nguyen

et al. 2022

Preprint

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Soil factors, especially metal elements in the soil, play a significant role in forming and accumulating secondary metabolites, which determine the medicinal properties of medicinal herbs. In this study, the concentrations of some metal elements (K, Mn, Fe, Cu, Zn, and Cr) in Cam Mountain and Tinh Bien Town, An Giang Province, Vietnam, were determined using the XRF method. We simultaneously determined the total phenolic and flavonoid content of some medicinal herbs collected from the collected soil sample areas, thereby assessing the influence of these elements on the formation of secondary metabolites in medicinal plants. The results showed that K, Mn, and Cr were mainly concentrated in the topsoil and transition layers; Fe and Cu elements tended to concentrate in the transition layer and the subsoil when surveying the soil profile. K, Mn, Cu, and Cr concentrations were more focused in Tinh Bien area, while Fe and Zn had higher concentrations at Cam Mountain. Additionally, results from evaluating the relationship between the content of the elements in the soil and the content of two active compounds also showed the corresponding relationship between Cr/Phenolic, Cu/Phenolic, and Zn/Flavonoid (R2 = 0.74; R2 = 0.75; R2 = 0.90, respectively) with the corresponding correlation equations at level six.

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Phenolic compounds in leaves ofSalixspecies and hybrids growing under different soil conditions

Cited by 23 publications

References 43 publications

Laccase activity of the ascomycete fungus Nectriella pironii and innovative strategies for its production on leaf litter of an urban park

Laccase activity of the ascomycete fungus Nectriella pironii and innovative strategies for its production on leaf litter of an urban park

Antioxidant Activity and Chemical Characteristics of Supercritical CO2 and Water Extracts from Willow and Poplar

Evaluating the Content of Some Metal Elements in Soil and Their Effects on the Total Phenolic and Flavonoid Contents of Some Medicinal Plants Using X-Ray Fluorescence (XRF) Method

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