Phenolic Compounds in Chilean Mistletoe (Quintral, Tristerix tetrandus) Analyzed by UHPLC–Q/Orbitrap/MS/MS and Its Antioxidant Properties

Abstract

Abstract:Mass spectrometry has become a method of choice to characterize bioactive compounds in biological samples because of its sensitivity and selectivity. Hybrid ultra-HPLC hyphenated with Orbitrap mass analyzer is an innovative state of the art technology that allows fast and accurate metabolomic analyses. In this work the metabolites of a Chilean mistletoe endemic to the VIII region of Chile were investigated for the first time using UHPLC mass analysis (UHPLC-PDA-HESI-Orbitrap MS n ). The anthocyanins, together with the non-pigmented phenolics were fingerprinted and correlated with the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics, flavonoids and anthocyanins measured by spectroscopic methods. Six anthocyanins were identified, and among them, the 3-O-glycosides of delphinidin and cyanidin were the major ones. In addition, several phenolic acids (including feruloylquinic acid, feruloyl glucose, chlorogenic acid) and several flavonols (luteolin, quercetin, apigenin, isorhamnetin and glycoside derivatives) were also identified. The mistletoe leaves showed the highest antioxidant activity as measured by the DPPH radical bleaching, ferric reducing antioxidant power and superoxide anion scavenging activity tests (13.38˘0.47 µg/mL, 125.32˘5.96 µmolTE/g DW and 84.06˘4.59 at 100 µg/mL, respectively).

An aqueous polyphenol extract from Rosa rugosa tea has antiaging effects on Caenorhabditis elegans

“…According to our results, 23 kinds of polyphenols were separated and identified, with quercetin and its glycosides being the principal component, having a relative percentage content of 25.93% in total. Our results were completely in accordance with some previous reports (Mario et al, 2016;Riffault et al, 2014;Song et al, 2016;Zhu et al, 2018). So, we used quercetin as the positive control in the following experiments of RAP activity determination.…”

“…The identification of these molecules was confirmed through a series of characteristic ion fragments, which was showed in Table 1 in detail. According to the fragmentation mechanisms of polyphenols (Mario, Cristina, Carlos, & Sepúlveda, 2016;Song et al, 2016;Zhu et al, 2018), a tentative identification of 23 compounds of RAP was performed. For example, a mass fragments of 169 u (C 7 H 5 O 5 ) was detected when a galloyl unit was lost from the [M-H] − ion (Number 1,2,3,4,14).…”

Chemical Composition and Antioxidant Activity of Aloe vera from the Pica Oasis (Tarapacá, Chile) by UHPLC-Q/Orbitrap/MS/MS

“…In this scenario, the use of state-of-the-art tools such as metabolomic mass fingerprinting can help to study the metabolomic processes in extreme systems like the one occurring among the "biodiversity of the Atacama Desert." For a complete chemical characterization of A. vera from Tarapaca Region, we used high-resolution hyphenated LC-MS (UHPLC-MS) techniques whose advantage is the rapid separation of compounds and the most accurate determination of the masses [21]. is technique is considered gold standard for the analysis of phenolic compounds, due to its versatility, precision, and relatively low cost [22].…”

High Resolution UHPLC-MS Metabolomics and Sedative-Anxiolytic Effects of Latua pubiflora: A Mystic Plant used by Mapuche Amerindians

“…In the same manner, peaks 27, 30 and 32 all with full MS anions at around 477.10273 amu, and producing all MS n fragments (315.05093, 300.02798, 271.02490 amu) diagnostics of quercetin methyl ester (rhamnetin or isorhamnetin) were identified as the isomers isorhamnetin-3-O-glucoside, isorhamnetin-3-O-galactoside and rhamnetin-3-O-glucoside (C 22 H 21 O 12 ) (Simirgiotis et al, 2016a) respectively. Peak 31 with a [M-H] − ion at m/z: 461.10892 was identified as isorhamnetin-3-O-rhamnoside (C 22 H 21 O 11 ) (Brito et al, 2014) and peak 33 as the aglycone of the mentioned compounds isorhamnetin (C 16 H 11 O 7 ).…”