Phenolic Compounds in Centaurea rupestris Tissues and Their Antiphytoviral Activity

Abstract

Abstract. Quantitative and qualitative analysis of phenolic compounds in tissue extracts from Centaurea rupestris L. as well as their antiphytoviral activity against Tomato bushy stunt virus was performed. Extracts of flowers, leaves and roots from C. rupestris growing in the wild, as well as extracts from shoots, tissue consisting of callus and shoots and undifferentiated callus grown in vitro, were tested. Between tested extracts predominantly quantitative and only several qualitative differences in phenolics were detected by high performance liquid chromatography. The highest amounts of quercetagetin 3'-methylether-7-O-ß-D-glucopyranoside and quercetin were detected in flowers while in leaves the highest amounts of luteolin, caffeic and p-coumaric acid were detected. Except for roots, antiphytoviral activity of all other extracts was high, inducing virus inhibition, ranging from 43 to 90 %. Simultaneous application of quercetin, caffeic or p-coumaric acid with virus decreased the number of lesions indicating that these substances contribute to the antiphytoviral activity of C. rupestris extracts. (doi: 10.5562/cca2272)

Terezine E, bioactive prenylated tryptophan analogue from an endophyte of Centaurea stoebe

“…The anticancer activity of extracts from Centaurea species has been furthermore confirmed by a study conducted on the Turkish endemic plant C. drabifolia which revealed antiproliferative activity against leukemia cells (Formisano et al2017). Previous studies also reported anti-inflammatory activities of C. sadleriana (Csupor et al 2013) and more recently antiphytoviral activity against Tomato bushy stunt virus for phenolic compounds isolated from C. rupestris (Curkovic-Perica et al 2014).…”

Phytochemical and Cytogenetic Study of Two Centaurea Species from Croatia: the Particular Case of Diploid and Tetraploid C. salonitana

“…[15 -19] Centaurea salonitana is known for its anti-inflammatory and wound healing properties, [20] while the phenolic compounds of C. rupestris have shown good antiphytoviral activity, along with the antimicrobial activity of the essential oil tested previously. [21,22] Plant secondary metabolites mixtures, such as volatile oils (VOs), may contain chemical compounds that are characteristic of certain species and whose study is of interest for phytotaxonomy. The common constituents of the Centaurea species' volatile oils belong to a group of non-oxygenated and oxygenated terpenes, together with the non-oxygenated hydrocarbon derivatives, while oxygenated hydrocarbon derivatives make up a smaller part of VOs.…”

“…Many Centaurea species are known to be valuable sources of pharmacologically active compounds and have been used in traditional medicine to treat various diseases and ailments [15–19] . Centaurea salonitana is known for its anti‐inflammatory and wound healing properties, [20] while the phenolic compounds of C. rupestris have shown good antiphytoviral activity, along with the antimicrobial activity of the essential oil tested previously [21,22] …”

“…The common constituents of the Centaurea species’ volatile oils belong to a group of non‐oxygenated and oxygenated terpenes, together with the non‐oxygenated hydrocarbon derivatives, while oxygenated hydrocarbon derivatives make up a smaller part of VOs. The main constituents of the Centaurea essential oils are the sesquiterpenes germacrene D, bicyclogermacrene, β‐caryophyllene, caryophyllene oxide, spathulenol, and α ‐copaene [21–25,31–48] …”