Phenolic compounds from Brazilian propolis with pharmacological activities

Marcucci, María Cristina; Ferreres, Federico; Garcı́a-Viguera, C.; Bankova, Vassya; Castro, Solange L. de; Dantas, A.P.; Valente, Patricia; Paulino, Niraldo

doi:10.1016/s0378-8741(00)00326-3

Cited by 426 publications

(377 citation statements)

References 35 publications

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“…Although differences in susceptibility to propolis in the bacteria tested resulted in MBC values ranging from 2.5 and 20.0 mg/mL, one (1) determined the activity of 2,2 dimethyl-6-carboxyethenyl-2H-1-benzopyran, 3-prenyl-4-hydroxycinnamic acid and 3,5-diprenyl-4-hydroxycinnamic acid against a series of bacteria. Marcucci et al (13), have determined the antibacterial activity of 2,2 dimethyl-6-carboxyethenyl-2H-1-benzopyrane, 6-propenoic-2, 2-dimethyl-8-prenyl-2H-1-benzopyranic acid and 3,5-diprenyl-4-hydroxycinnamic acid against S. aureus.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of the composition of Brazilian propolis extracts by chromatography and evaluation of their in vitro activity against gram-positive bacteria

Sawaya¹,

Souza²,

Marcucci³

et al. 2004

Braz. J. Microbiol.

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Brazilian propolis from São Paulo state was submitted to extraction using several solvents, resulting in extracts with different composition. These extracts were submitted to Thin Layer Chromatography (TLC). Bioauthographic analysis of the TLC plates identified fractions with inhibitory activity, which were then analysed by High Performance Liquid Chromatography (HPLC). In vitro assays, commonly used to evaluate the activity of propolis against Gram-positive bacteria, were compared to determine which rendered the most consistent results. The bactericidal activity of these extracts were analysed by Serial Dilution in Tubes and Agar Plate Diffusion. Serial Dilution in Tubes obtained the most consistent results, with the Minimal Bactericidal Concentration of the extracts ranging between 2.5 and 20.0 mg/mL, for the species of Gram-positive bacteria tested. The results of the Agar Plate Diffusion were directly proportional to the hydro-solubility of the extracts and did not evaluate their bactericidal activity correctly. The bactericidal activity of this sample of propolis was due to the combined effect of several components that were identified by HPLC and were best extracted using 50% ethanol as a solvent.

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Section: Discussionmentioning

confidence: 99%

“…The elution was carried out with a linear gradient and a flow rate of 1 mL/min. The detection was monitored at 280 and 340 nm and the components identified by comparison with standards acquired commercially or isolated during previous work (13).…”

Section: High Performance Liquid Chromatographymentioning

confidence: 99%

Analysis of the composition of Brazilian propolis extracts by chromatography and evaluation of their in vitro activity against gram-positive bacteria

Sawaya¹,

Souza²,

Marcucci³

et al. 2004

Braz. J. Microbiol.

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“…Thus, our group has been engaged in experimental chemotherapy studies using propolis (Higashi & De Castro 1994, De Castro & Higashi 1995, Marcucci et al 2001, Prytzyk et al 2003, Paulino et al 2003, Salomão et al 2004). Propolis, a bee product collected from different plant exudates, is a complex mixture containing known bioactive constituents, such as flavonoids, phenolic acids and esters, terpenes, sesquiterpenes, aromatic aldehydes, and alcohols (Marcucci 1995 In temperate zones, bud exudates from poplar trees (Populus spp.)…”

Section: Extracts From a Bulgarian Propolis With Known Chemical Compmentioning

confidence: 99%

The effect of Bulgarian propolis against Trypanosoma cruzi and during its interaction with host cells

Dantas

Salomão

Barbosa

et al. 2006

Mem. Inst. Oswaldo Cruz

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Propolis has shown activity against pathogenic microorganisms that cause diseases in humans and animals. The ethanol (Et-Blg) and acetone (Ket-Blg) extracts from a Bulgarian propolis, with known chemical compositions, presented similar activity against tissue culture-derived amastigotes. The treatment of Trypanosoma cruzi-infected skeletal muscle cells with Et-Blg led to a decrease of infection and of the intracellular proliferation of amastigotes, while damage to the host cell was observed only at concentration 12.5 times higher than those affecting the parasite. Ultrastructural analysis of the effect of both extracts in epimastigotes revealed that the main targets were the mitochondrion and reservosomes. Et-Blg also affected the mitochondrion-kinetoplast complex in trypomastigotes, offering a potential target for chemotherapeutic agents.Key words: propolis -natural products -Trypanosoma cruzi -Chagas disease Chagas disease, caused by the trypanosomatid protozoan Trypanosoma cruzi, is endemic in Latin America. Initially restricted to natural ecotopes, with transmission among small autochthonous mammals by hematophagous Triatominae bugs, the occupation by humans of such ecotopes led to the establishment of the vectors in human dwellings, changing the characteristic of the disease from zoonotic to antropozoonotic. In humans, the acute phase results in 2-8% mortality, specially among children, while in the chronic phase most patients remain asymptomatic and about 30 to 40% of the cases develop symptoms of cardiac or digestive lesions. The World Health Organization estimates that about 18 million people are infected with T. cruzi (WHO 2002). The available drugs for the clinical treatment, the nitroderivatives benznidazole and nifurtimox, are unsatisfactory. They present variable healing effects during the acute phase according to the geographical region and require long term treatment, besides presenting frequent toxic side effects and limited efficacy in the chronic stage (reviewed in Coura & De Castro 2001).In this context, there is an intense search for new synthetic compounds and natural products for treatment of Chagas disease. Thus, our group has been engaged in experimental chemotherapy studies using propolis (Higashi & De Castro 1994, De Castro & Higashi 1995, Marcucci et al. 2001, Prytzyk et al. 2003, Paulino et al. 2003, Salomão et al. 2004). Propolis, a bee product collected from different plant exudates, is a complex mixture containing known bioactive constituents, such as flavonoids, phenolic acids and esters, terpenes, sesquiterpenes, aromatic aldehydes, and alcohols (Marcucci 1995). In temperate zones, bud exudates from poplar trees (Populus spp.) are the main source of flavonoid-rich propolis, which are rich in flavonoids (reviewed in Bankova et al. 2000).During the last decades, an increasing number of studies on the chemical composition, biological activity, and therapeutic uses of propolis has been published (reviewed in De Castro 2001). In recent studies with a Bulgarian propolis sample, we ...

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“…A ação antitumoral sugerida para a própolis de A. mellifera L. pode estar relacionada à sua composição química, que inclui, dentre outros compostos, flavonóides, ácidos fenólicos e terpenos (Kimoto et al, 1999;Marcucci et al, 2001). Quercetina e galangina são flavonóides que têm sido identificados como promissores agentes antitumorais (Orsolic et al, 2005a;b).…”

Section: Introductionunclassified

Efeito do tratamento com própolis de Scaptotrigona aff. postica sobre o desenvolvimento do tumor de Ehrlich em camundongos

Araújo¹,

Dutra²,

Costa³

et al. 2010

Rev. bras. farmacogn.

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RESUMO:Neste trabalho foi investigado o efeito do extrato hidroalcoólico de própolis (EHP) de Scaptotrigona aff. postica sobre o desenvolvimento do tumor de Ehrlich na forma sólida, sobre a celularidade dos órgãos linfóides dos animais portadores de tumor, bem como, sobre a produção de óxido nítrico (NO) pelos macrófagos destes animais. Camundongos Swiss foram divididos em quatro grupos: controle, EHP 0,5; EHP 5 e EHP 50, os quais foram tratados por via intraperitoneal com dose única de solução salina (NaCl 0,9%); 0,5; 5 ou 50 mg de EHP/kg de animal, respectivamente. Depois de 48 h do tratamento, os animais foram inoculados com 10 5 células do tumor de Ehrlich nas patas. Os resultados mostraram que o tratamento com EHP nas doses de 5 e 50 mg/kg inibiu de forma significativa o desenvolvimento do tumor a partir do 6º dia pós-inóculo quando comparado ao controle e ao EHP 0,5. Além disso, houve aumento significativo da celularidade do baço e da medula óssea nos grupos EHP 0,5 e EHP 5 em relação ao controle. A produção de NO estimulada com concanavalina A (ConA) apresentou uma significante diminuição nos grupos tratados com EHP em relação ao controle. Pode-se concluir que o tratamento com EHP apresentou efeito antitumoral quando administrado nas doses de 5 e 50 mg/kg, o que pode estar relacionado com a sua composição química e com a inibição da produção de NO.Unitermos: própolis, Scaptotrigona aff. postica, tumor de Ehrlich, abelhas sem ferrão, Meliponinae.ABSTRACT: "Effect of propolis of Scaptotrigona aff. postica on the development of the tumor of Ehrlich in mice". It was investigated the effect of hydroalcoholic extract (HEP) of propolis from Scaptotrigona aff. postica on the solid Ehrlich tumor, on the tumor-bearing mice lymphoid organs and on the nitric oxide (NO) production. Swiss mice were divided in 4 groups: control, HEP 0.5; HEP 5 and HEP 50 that was treated by intraperitoneal route with a single dose of saline solution (NaCl 0.9%) or 0.5 or 5 or 50 mg of HEP/kg body weight, respectively. After 48 h of treatment, the animals were inoculated with 10 5 tumor cells in their footpad. The results showed that the treatment with HEP in the doses of 5 and 50 mg/kg inhibited the development of the tumor from the 6 th day post inoculums when compared to the control and to the HEP 0.5 groups. Besides, there was an increase of spleen and bone marrow cell number in HEP 0.5 and HEP 5 as compared to the control. Concanavalin A (ConA)-stimulated NO production was decreased in all HEP-treated groups when compared to the control. In conclusion, the treatment with HEP had an anti-tumor effect what may be related to its chemical composition and to the inhibition of NO production.

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Phenolic compounds from Brazilian propolis with pharmacological activities

Cited by 426 publications

References 35 publications

Analysis of the composition of Brazilian propolis extracts by chromatography and evaluation of their in vitro activity against gram-positive bacteria

Analysis of the composition of Brazilian propolis extracts by chromatography and evaluation of their in vitro activity against gram-positive bacteria

The effect of Bulgarian propolis against Trypanosoma cruzi and during its interaction with host cells

Efeito do tratamento com própolis de Scaptotrigona aff. postica sobre o desenvolvimento do tumor de Ehrlich em camundongos

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