Phenolic Compounds, Antioxidant and Anti-Cancer Properties of the Australian Maroon Bush Scaevola spinescens (Goodeniaceae)

Vuong, Quan V.; Sadeqzadeh, Elham; Hirun, Sathira; Goldsmith, Chloe D.; Zammitt, Nicholas; Bowyer, Michael C.; Sakoff, Jennette A.; Thorne, Rick F.; Weidenhofer, Judith; Scarlett, Christopher J.

doi:10.4172/1948-593x.s12-002

Cited by 6 publications

(4 citation statements)

References 60 publications

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“…Furthermore, folic acid showed the lowest antioxidant capacity among the tested compound, 4% of that of ASC, which is in good accordance with a previous study . Regarding water-insoluble antioxidants, α-tocopherol showed 67% antioxidant activity of that of ASC, which is in good accordance with the literature . β-Carotene has about half of the antioxidant activity of ASC, which is very similar to that obtained previously by an ORAC assay …”

Section: Resultssupporting

confidence: 85%

“…40 Regarding water-insoluble antioxidants, α-tocopherol showed 67% antioxidant activity of that of ASC, which is in good accordance with the literature. 41 β-Carotene has about half of the antioxidant activity of ASC, which is very similar to that obtained previously by an ORAC assay. 42 As shown in Figure 3, the CL intensities obtained from ASC and dihydrolipoic acid were more intense than the CL obtained from their oxidized forms, dehydroascorbic acid and α-lipoic acid, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…The microplate-based CL imaging assay was applied to simultaneously evaluate the antioxidant capacity of many food samples. Aqueous extracts of food samples are widely used for evaluation of antioxidant capacities of food samples ,− because of the simplicity of the extraction and for more simulation of the obtained antioxidant value upon the consumption of these food samples.…”

Section: Resultsmentioning

confidence: 99%

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A Smart Advanced Chemiluminescence-Sensing Platform for Determination and Imaging of the Tissue Distribution of Natural Antioxidants

et al. 2020

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Antioxidants have gained marked attention owing to their ability to prevent the oxidation of biological components and to protect the body from reactive oxygen species, thereby maintaining human health. Thus, antioxidant-rich dietary supplements and natural foods can be effective against oxidative stress and can even act as chemopreventive agents. Therefore, a simple and rapid assay for evaluation of antioxidant capacity and assessment of their distribution profile in natural sources is vital. Herein, we report a rapid, innovative chemiluminescence (CL) platform for evaluation and visualization of antioxidant capacity. We found that intense and long-lasting CL was formed upon the redox reaction of quinones, e.g., menadione, with antioxidants, e.g., l-ascorbic acid, in the presence of luminol. The produced CL intensities were proportional to the antioxidants’ concentrations with a detection limit of 0.18 μM for the model antioxidant, l-ascorbic acid. As the formed CL was long-lasting, it could be easily captured and detected with a charge-coupled device (CCD) camera. To evaluate the quantification ability of the CCD camera, we developed a smart and fast microplate-based assay based on photographing the generated CL with a cooled CCD camera. The photographed CL intensities were linearly proportional with the antioxidant concentrations, and then the method was applied for photographing multiple food sample extracts. Ultimately, we utilized our method for the distribution profiling of antioxidant capacity in food cut sections. Samples were dipped in luminol and then in quinone, followed by CCD camera photography, without the need for any pulverization/extraction procedure, giving precise antioxidant distribution information.

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Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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A Smart Advanced Chemiluminescence-Sensing Platform for Determination and Imaging of the Tissue Distribution of Natural Antioxidants

et al. 2020

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“…Regarding the concentration that inhibits cell growth by 50% (GI 50 ), the lowest GI 50 value was observed for A2780 (31 lg/ml), followed by SMA (40 lg/ml), and HT29 (47 lg/ml), whereas the highest GI 50 value was determined for SJ-G2 (81 lg/ ml). Cell growth inhibition by the PTL extract achieved in this study was stronger than those of the extracts from E. robusta leaf [22] and maroon bush (S. spinescens) [23] (both <100% on the same cancer cell lines), while the GI 50 value of the PTL extract on all cancer cell lines obtained in this study was also lower than those of the extracts from E. robusta leaf (77 -183 lg/ml) and S. spinescens (28 -110 lg/ml, except for MCF-7: 12 lg/ml and SJ-G2: 20 lg/ml), and being comparable to the P. trimera root extract. [9] The high saponin content of PTL might contribute to its strong anti-proliferative capacity when compared to those of E. robusta leaf and S. spinescens.…”

Section: Resultsmentioning

confidence: 94%

Physicochemical Properties, Antioxidant and Anti‐proliferative Capacities of Dried Leaf and Its Extract from Xao tam phan (Paramignya trimera)

Nguyen

Sakoff

Scarlett

2017

Chemistry & Biodiversity

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Xao tam phan (Paramignya trimera) has been used for the treatment of cancer and cancer-like aliments. Among different parts of the P. trimera plant, leaf is considered as a residual part after harvesting of the root. This study aimed to determine the physiochemical properties and the antioxidant and anti-proliferative capacities of P. trimera leaf (PTL) using microwave drying for the preparation of dry sample; MeOH and microwave-assisted extraction for the preparation of crude extract; and freeze-drying for the preparation of powdered extract. The results showed that total phenolic, total flavonoid, proanthocyanidin, and saponin contents of PTL prepared by microwave drying at 450 W were 25.4 mg gallic acid equiv. (GAE), 86.3 mg rutin equiv. (RE), 5.6 mg catechin equiv. (CE), and 702.1 mg escin equiv. (EE) per gram dried sample, respectively. Gallic acid, protocatechuic acid, ellagic acid, rutin, and quercetin were identified in the PTL MeOH extract. Dried PTL displayed potent antioxidant activity, while the powdered PTL extract exhibited great anti-proliferative capacity on various cancer cell lines including MiaPaCa-2 (pancreas), HT29 (colon), A2780 (ovarian), H460 (lung), A431 (skin), Du145 (prostate), BE2-C (neuroblastoma), MCF-7 (breast), MCF-10A (normal breast), and U87, SJ-G2, and SMA (glioblastoma). Anti-proliferative capacity on pancreatic cancer cells (MiaCaPa2, BxPc3, and CFPAC1) of PTL extract (200 μg/ml) was significantly higher (P < 0.05) than those of ostruthin (20 μg/ml) and gemcitabine (50 nm), and to be comparable to the powdered P. trimera root extract and a saponin-enriched extract from quillajia bark (a commercial product). The findings from this study allow us to conclude that the PTL is a rich source of phytochemicals that possess promising antioxidant and anti-proliferative activities, therefore it shows potential as lead compounds for application in the nutraceutical, medicinal and pharmaceutical industries.

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Physicochemical, Antioxidant, and Cytotoxic Properties of Xao Tam Phan (Paramignya trimera) Root Extract and Its Fractions

Nguyen

Sakoff

Scarlett

2017

Chemistry & Biodiversity

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Xao tam phan (Paramignya trimera (Oliv.) Guillaum) has been used as a medicinal plant for cancer prevention and treatment in recent years. The objective of this study was to determine the physicochemical, antioxidant, and cytotoxic properties of crude P. trimera root (PTR) extract and its fractions using MeOH as a solvent and microwave-assisted extraction as an advanced technique for preparation of the PTR extract. The results showed that the PTR extract had high contents of saponins, phenolics, flavonoids, and proanthocyanidins (7731.05 mg escin equiv. (EE), 238.13 mg gallic acid equiv. (GAE), 81.49 mg rutin equiv., and 58.08 mg catechin equiv. (CE)/g dried extract, resp.). Antioxidant activity of PTR extract was significantly higher (P < 0.05) than those of four its fractions and ostruthin, a key bioactive compound in the P. trimera, while potent cytotoxic capacity of PTR extract on various cancer cell lines in terms of MiaPaCa-2 (pancreas), HT29 (colon), A2780 (ovarian), H460 (lung), A431 (skin), Du145 (prostate), BE2-C (neuroblastoma), MCF-7 (breast), MCF-10A (normal breast), and U87, SJ-G2, SMA (glioblastoma) was observed with GI values ranging from 15 to 32 μg/ml. Cytotoxic potential on pancreatic cancer cells of PTR extract (100 - 200 μg/ml) was significantly higher (P < 0.05) than those of its four fractions (50 μg/ml), ostruthin (20 μg/ml) and gemcitabine (50 nm), and being comparable to a saponin-enriched extract from quillajia bark, a commercial product. Based on the results achieved, we can conclude that the PTR extract is a potential source for application of in the nutraceutical, medical, and pharmaceutical industries.

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Phenolic Compounds, Antioxidant and Anti-Cancer Properties of the Australian Maroon Bush Scaevola spinescens (Goodeniaceae)

Cited by 6 publications

References 60 publications

A Smart Advanced Chemiluminescence-Sensing Platform for Determination and Imaging of the Tissue Distribution of Natural Antioxidants

A Smart Advanced Chemiluminescence-Sensing Platform for Determination and Imaging of the Tissue Distribution of Natural Antioxidants

Physicochemical Properties, Antioxidant and Anti‐proliferative Capacities of Dried Leaf and Its Extract from Xao tam phan (Paramignya trimera)

Physicochemical, Antioxidant, and Cytotoxic Properties of Xao Tam Phan (Paramignya trimera) Root Extract and Its Fractions

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