“…Nevertheless, the obtained range of TPC (from 307.01 ± 4.77 to 1273.75 ± 6.25 mg GAE/kg) was comparable with the results obtained for buckwheat honey (26), which is recognizable by its specific dark color. These values were lower than the published TPC values for polyfloral and monofloral Algerian honey (14).…”
Section: Antioxidant Capacity Of Honey Samplescontrasting
confidence: 80%
“…The parameters determined by sensory analyses can be dependent on the chemical constituents and their content in the honey, which was shown for the honey color and phenolic content (11), as well as for the botanical origin of honey (25). The color of the honey is related to the composition of phenolic compounds (14,16), with total flavonoid content showing the highest influence (25). A correlation between phenolic composition and antioxidant properties of honey (16) was also noted.…”
Section: Introductionmentioning
confidence: 88%
“…Given that Serbia (8)(9)(10) and its neighboring countries (11)(12)(13) have a high potential for honey production, testing of honey is of high importance. Based on published studies the phenolic compounds play a significant role in the bioactive properties of honey (8,10,11,14), and can also serve as potential markers of the botanical and/or geographical origin of honey (10,12,15). Determination of physicochemical parameters has proven to be an additional tool for differentiating honey types (8,9,(16)(17)(18)(19) and a good indicator of honey quality (20), which is also dependent on storage conditions that also affect the phenol content (21).…”
Section: Introductionmentioning
confidence: 99%
“…A correlation between phenolic composition and antioxidant properties of honey (16) was also noted. Antimicrobial activity against Staphylococcus aureus was observed for many types of honey (14). It was found that the antibacterial and antioxidant properties of honey mostly depend on the geographical origin and to a lesser extent on the type of honey (14).…”
This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13–4.94), diastase activity (24.20–41.70 DN), acidity (14.60–29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20–3.90 g/100 g), and moisture content (15.01–19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.
“…Nevertheless, the obtained range of TPC (from 307.01 ± 4.77 to 1273.75 ± 6.25 mg GAE/kg) was comparable with the results obtained for buckwheat honey (26), which is recognizable by its specific dark color. These values were lower than the published TPC values for polyfloral and monofloral Algerian honey (14).…”
Section: Antioxidant Capacity Of Honey Samplescontrasting
confidence: 80%
“…The parameters determined by sensory analyses can be dependent on the chemical constituents and their content in the honey, which was shown for the honey color and phenolic content (11), as well as for the botanical origin of honey (25). The color of the honey is related to the composition of phenolic compounds (14,16), with total flavonoid content showing the highest influence (25). A correlation between phenolic composition and antioxidant properties of honey (16) was also noted.…”
Section: Introductionmentioning
confidence: 88%
“…Given that Serbia (8)(9)(10) and its neighboring countries (11)(12)(13) have a high potential for honey production, testing of honey is of high importance. Based on published studies the phenolic compounds play a significant role in the bioactive properties of honey (8,10,11,14), and can also serve as potential markers of the botanical and/or geographical origin of honey (10,12,15). Determination of physicochemical parameters has proven to be an additional tool for differentiating honey types (8,9,(16)(17)(18)(19) and a good indicator of honey quality (20), which is also dependent on storage conditions that also affect the phenol content (21).…”
Section: Introductionmentioning
confidence: 99%
“…A correlation between phenolic composition and antioxidant properties of honey (16) was also noted. Antimicrobial activity against Staphylococcus aureus was observed for many types of honey (14). It was found that the antibacterial and antioxidant properties of honey mostly depend on the geographical origin and to a lesser extent on the type of honey (14).…”
This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13–4.94), diastase activity (24.20–41.70 DN), acidity (14.60–29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20–3.90 g/100 g), and moisture content (15.01–19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.
“…In our study, S. pneumoniae (Gram+) was more sensitive compared to P. aeruginosa (Gram−) both in the time-kill assay and membrane degradation studies, which is likely due to structural differences. Similarly, Otmani et al [81] found that a Gram+ strain (Staphylococcus aureus FRI S6) was inhibited to larger extent by honey samples, compared to a Gram-strain (Salmonella typhi ATCC 14028).…”
Honey is a rich source of carbohydrates, while minor compounds such as amino acids and polyphenols contribute to its health-promoting effects. Honey is one of the oldest traditional remedies applied for microbial infections, due to its antibacterial, anti-inflammatory, and antioxidant properties. The aim of this study was to investigate the antibacterial and antibiofilm effects of Hungarian black locust, linden, and sunflower honeys against the most common biofilm-forming respiratory tract pathogens Haemophilus spp., Pseudomonas aeruginosa, and Streptococcus pneumoniae. The unifloral character of all three honey types was confirmed by melissopalynological analysis. The antibacterial activity of each honey sample against each bacterium strain was proven with agar well diffusion assay and thin layer chromatography—direct bioautography. Kinetics and mechanisms of antibacterial action were clarified with time-kill assay and membrane degradation study. The anti-biofilm activity was evidenced using crystal violet assay. In each assay, linden honey was the most effective, followed by sunflower and black locust honey. In addition, each honey sample had greater potential to suppress respiratory tract bacteria, compared to major sugar components. In conclusion, honey in general and linden honey in particular, can have a role in the treatment of respiratory tract infections caused by biofilm-forming bacteria.
Robusta coffee blossom honey stands as a key regional product in Dak Lak province, Vietnam. Despite its significance, there exists a dearth of scientific data for assessing its quality. This study aims to fill this gap by characterizing the physicochemical properties and biological activities of coffee blossom honeys from three distinct sub‐regions within Dak Lak province, Vietnam. These activities include ferric reducing power (FRP), DPPH and ABTS radical scavenging, as well as tyrosinase inhibitory activities. Moreover, the study compares these honey samples with other popular varieties in Vietnam, such as Lychee and Longan honeys. The physicochemical parameters of the honey samples meet the standards set by Codex Alimentarius 2001. Through UPLC analysis, eleven compounds were identified, with caffeine serving as a marker for coffee honey. Furthermore, by employing multiple factor analysis (MFA), it was observed that certain physicochemical properties correlate positively with tyrosinase inhibitory, DPPH, ABTS free radicals scavenging activities, and FRP. Notably, tyrosinase inhibitory activity exhibited a positive correlation with antioxidant activity. These findings underscore the high quality of Coffea robusta honey, showcasing its potent antioxidant and tyrosinase inhibitory activities.
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