Phenolic Composition, Antioxidant and Enzyme Inhibitory Activities of Eryngium bornmuelleri leaf

Abstract

Eryngium bornmuelleri Nab. (Tusî) is an endemic botanical from the Eastern Anatolia region of Turkey traditionally used for preparation of herbal tea. Within this study, phenolic composition, antioxidant capacities and inhibitory activities towards selected digestive enzymes of E. bornmuelleri leaf were investigated. Sequential extracts, obtained by extraction of plant tissue by ethanol, acetone and water exhibited pronounced antioxidant capacities and in a dose-dependent manner suppressed the metabolic syndrome related enzymes: α-amylase, α-glucosidase and pancreatic lipase. All extracts contained high levels of phenolic compounds. Flavonoid glycosides were the main phytochemicals detected, with rutin as the major compound (70% of total phenolics). Chlorogenic, hydroxybenzoic and caftaric acids as well as traces of caffeic, ferulic and rosmarinic acids were also detected. Correlation analysis indicated that phenolic compounds were the major sources of the enzyme-inhibitory activities. This study suggests that E. bornmuelleri leaf extracts can modulate the metabolism of sugars and fats through inhibition of the relevant digestive enzymes.

Phytochemical and biological evaluations of Arum hygrophilum boiss. (Araceae)

“…[313233] Additionally, the efficacy of phenolic acids, namely caffeic-, rosmarinic-, and ferulic acids, as PL inhibitors are well described. [3435] In effect, the results of the present study indicate that the PL inhibitory efficacy of A. hygrophilum may be attributable to their multiple phenolic components acting additively or synergistically. [36]…”

Phenolic composition and antioxidant properties of Eryngium maritimum (sea holly)

“…In addition, Dalar et al [5] have suggested that rutin, quercetin, luteolin and caffeic acid obtained from E. bornmuelleri leaf extract may be responsible for their antioxidant properties.…”

Effects of Fruit Extracts on Pancreatic Lipase Activity in Lipid Emulsions

“…Other authors also observed significantly weaker inhibitory activity of plant extract as well as pure phenolic compounds against pancreatic lipase than that of orlistat [21][22][23]. For example, the IC 50 values for some Mediterranean dietary plants ranged from 0.99 to > 2.5 mg/ml while orlistat showed the IC 50 value of 0.018 mg/ml [23]. Cha et al [22] noted that (−)-epigallocatechin gallate showed the anti-lipase activity similar to that of orlistat with IC 50 =1.8 and 1.2 μM, respectively.…”

“…The total phenolic content in chokeberry was 1.04 %, while other fruits contained under 0.35 % of phenolic compounds (Table 4). Other authors also observed significantly weaker inhibitory activity of plant extract as well as pure phenolic compounds against pancreatic lipase than that of orlistat [21][22][23]. For example, the IC 50 values for some Mediterranean dietary plants ranged from 0.99 to > 2.5 mg/ml while orlistat showed the IC 50 value of 0.018 mg/ml [23].…”