Phenolic Composition and Biological (Anti Diabetic and Antioxidant) Activities of Different Solvent Extracts of an Endemic Plant (Heliotropium Strigosum)

Abstract

Heliotropium strigosum plant is widely used in traditional medicines for treatment of various ailments. In current study, an effort was made to evaluate phenolic profile as well as antidiabetic and antioxidant activities of crude methanol extract and different solvent (n-hexane, ethyl acetate, chloroform and aqueous) fractions of H. strigosum. Total phenolic contents were determined by spectrophotometric assay. Polyphenolic compounds in crude methanol extract and each solvent fraction were identified by reverse phase high performance liquid chromatography (RP-HPLC). Antioxidant and antidiabetic activities were determined using DPPH and α-amylase inhibition assays, respectively. The outcome of spectrophotometric assay showed that methanol extract had higher amount of total phenolic (84.50 ± 0.06 µg GAE/ mg of plant extract) contents than aqueous, ethyl acetate, chloroform and n-hexane fractions, respectively. The RP-HPLC analysis revealed the maximum number of phenolic (chromotropic acid, quercetin, trans-4-hydroxy-3-methoxy cinnamic acid, vanillic acid, gallic acid, caffeic acid, m-coumaric acid, p-coumaric acid, syringic acid, sinapic acid and ferulic acid) compounds in methanol extract. Methanol extract (IC 50 = 8.97 µg/mL) exhibited the maximum antidiabetic activity followed by aqueous (IC 50 = 20.04 µg/mL), ethyl acetate (IC 50 = 27.79 µg/mL), chloroform (IC 50 = 56.87 µg/mL) and hexane (IC 50 = 32.16 µg/mL) fractions, respectively. The outcome of antioxidant assay revealed that methanol extract was the leading one with regard to antioxidant activity at different doses (10 to 250 µg/mL). The current study concludes that H. strigosum solvents extracts with significant phenolic profile and potent biological activities could be explored for potential uses in neutraceutical and pharmaceutical industries.

Pharmacologically active flavonoids from the anticancer, antioxidant and antimicrobial extracts of Cassia angustifolia Vahl

“…Extensive researches on medicinal plants have also indicated that they are good sources of antioxidants [4–6]. They are involved in free radical scavenging activities which contribute to the protection from oxidative stress caused by the overproduction of free radicals and reactive oxygen species [7].…”

RP-UHPLC-MS Chemical Profiling, Biological and In Silico Docking Studies to Unravel the Therapeutic Potential of Heliotropium crispum Desf. as a Novel Source of Neuroprotective Bioactive Compounds

“…These results offer a better understanding of the antioxidant potential of H. crispum . In the previously reported data [ 14 ], the H. strigosum methanolic extract also showed higher potential in the DPPH assay, with IC 50 of 10.45 μg/mL having higher phenolic contents (84.50 ± 0.06 µg QE/mg of plant extract). In another report, the major product of the dichloromethane extract of H. ovalifolium has been reported to contain heliotropamide, an alkaloid with a novel oxopyrrolidine-3-carboxamide central moiety, which exhibited radical-scavenging properties in the DPPH test [ 23 ].…”

“…In the present study, a methanolic extract of H. crispum showed high total phenolic contents (24.84 mg GAE/g extract) compared to the flavonoid contents (19.73 mg GAE/g extract), as presented in Table 1 . In a published report on the outcome of a spectrophotometric assay of H. strigosum , the methanol extract showed a higher amount of total phenolic contents than the aqueous, ethyl acetate, chloroform, and n -hexane fractions [ 14 ]. Likewise, another study conducted on different extracts of H. indicum showed a higher amount of phenolic contents in a hydro-alcoholic crude extract compared with the methanolic, ethyl acetate, and hexane fractions [ 15 ].…”

“…The tentatively identified plant pyrrolizidine alkaloids (in positive ionization mode) were supinine ( 3 ), europine (4) , and heliotrine (5) [ 14 ]. These pyrrolizidine alkaloids ( Scheme 2 ) are known phytochemicals from various Heliotropium species.…”

Effects of Heliotropium strigosum and Trapa bicornis in hyperactive gut disorders

“…Trachelanthamidine and 3 phthalic acid esters have been isolated from H. strigosum. Similarly, chromotropic acid, quercetin, trans -4-hydroxy-3-methoxy cinnamic acid, vanillic acid, gallic acid, caffeic acid, m-coumaric acid, p-coumaric acid, syringic acid, sinapic acid and ferulic acid have also been detected in this plant (Qayyum et al, 2016).…”