Phenolic Composition and Antioxidant Capacity of Bilberry (Vaccinium myrtillus) Leaves in Northern Europe Following Foliar Development and Along Environmental Gradients

Martz, Françoise; Jaakola, Laura; Julkunen‐Tiitto, Riitta; Stark, Sari

doi:10.1007/s10886-010-9836-9

Cited by 109 publications

(110 citation statements)

References 37 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several studies demonstrated strong antioxidant activity of these phenolic compounds (Nakajima et al, 2004;Viljanen et al, 2004;Ehala et al, 2005). Recent studies confirm these findings also for phenolic composition and antioxidant capacity of bilberry leaves (Martz et al, 2010). Literature data indicate that the V. myrtillus is a medicinal plant in traditional medicine and it is applied as a source of active substances (Taruscio et al, 2004;Faria et al, 2005;Cooke et al, 2006).…”

Section: Discussionmentioning

confidence: 62%

Antibacterial and antioxidant activities of bilberry (Vaccinium myrtillus L.) in vitro

Vui¹,

Petkovi²,

Rodi-Grabovac³

et al. 2013

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Antibacterial and antioxidant activity, total phenolic and flavonoid concentrations of water, ethanol and ethyl acetate extract of fruits and leaves of Vaccinium myrtillus L. were studied. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) have been determined. Testing was performed on 30 clinical isolates, including strains of Escherichia coli, Enterococcus faecalis and Proteus vulgaris. The values for MIC were in the range from 5 to 40 mg/ml. The most sensitive bacterial strain was Enterococcus faecalis MF-Ef8 strain. The ethanol extract of fruits of V. myrtillus was found the most active. The total phenolic content was determined using Folin-Ciocalteu reagent and ranged between 31.44 to 119.17 mg GAE/g. The concentration of flavonoids in extracts was determined and the highest amount was in ethyl acetate extract of leaves of V. myrtillus. Antioxidant activity was monitored spectrophotometrically using 2,2-diphenyl-1-picrylhydrazyl (DPPH) reagent. The highest capacity to neutralize DPPH radicals (94%RSA) was found in the ethanol extract from fruits and in the water extract from leaves of V. myrtillus. The results of the total phenolic content determination of the examined extracts indicate that bilberry extracts are a rich source of phenolic compounds and also possess a significant antioxidant activity and moderate antibacterial activity.

show abstract

Section: Discussionmentioning

confidence: 62%

Antibacterial and antioxidant activities of bilberry (Vaccinium myrtillus L.) in vitro

Vui¹,

Petkovi²,

Rodi-Grabovac³

et al. 2013

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…Another explanation for the lack of correlation between changes in gene expression and metabolite accumulation in transgenic seedlings might be the seasonal regulation of flavan-3-ol biosynthesis. In bilberry (Vaccinium myrtillus), for example, gene expression and flavonoid biosynthesis occurred predominantly early in the growth season, after which the PA contents remained stable (Martz et al, 2010). Because, in our study, spruce saplings were harvested 4 months after their spring flush, a similar seasonal rhythm could have led to differences in gene expression later in the season, with larger differences in metabolite accumulation that reflect early season differences in biosynthetic rate.…”

Section: Discussionmentioning

confidence: 83%

Flavan-3-ols in Norway Spruce: Biosynthesis, Accumulation, and Function in Response to Attack by the Bark Beetle-Associated Fungus Ceratocystis polonica

et al. 2014

View full text Add to dashboard Cite

Proanthocyanidins (PAs) are common polyphenolic polymers of plants found in foliage, fruit, bark, roots, rhizomes, and seed coats that consist of flavan-3-ol units such as 2,3-trans-(+)-catechin and 2,3-cis-(–)-epicatechin. Although the biosynthesis of flavan-3-ols has been studied in angiosperms, little is known about their biosynthesis and ecological roles in gymnosperms. In this study, the genes encoding leucoanthocyanidin reductase, a branch point enzyme involved in the biosynthesis of 2,3-trans-(+)-flavan-3-ols, were identified and functionally characterized in Norway spruce (Picea abies), the most widespread and economically important conifer in Europe. In addition, the accumulation of flavan-3-ols and PAs was investigated in Norway spruce saplings after wounding or inoculation with the fungal pathogen Ceratocystis polonica, which is vectored by bark beetles (Ips typographus) and is usually present during fatal beetle attacks. Monomeric and dimeric flavan-3-ols were analyzed by reverse-phase high-pressure liquid chromatography, while the size and subunit composition of larger PAs were characterized using a novel acid hydrolysis method and normal phase chromatography. Only flavan-3-ol monomers with 2,3-trans stereochemistry were detected in spruce bark; dimeric and larger PAs contained flavan-3-ols with both 2,3-trans and 2,3-cis stereochemistry. Levels of monomers as well as PAs with a higher degree of polymerization increased dramatically in spruce bark after infection by C. polonica. In accordance with their role in the biosynthesis of 2,3-trans-(+)-flavan-3-ols, transcript abundance of Norway spruce LEUCOANTHOCYANIDIN REDUCTASE genes also increased significantly during fungal infection. Bioassays with C. polonica revealed that the levels of 2,3-trans-(+)-catechin and PAs that are produced in the tree in response to fungal infection inhibit C. polonica growth and can therefore be considered chemical defense compounds.

show abstract

“…[9] A previous study demonstrated that higher light intensity seemed to be a more important factor stimulating higher production of phenolics than the soil nutrient status. [31] Petunidin-3-O-glucoside (A7) was the most abundant anthocyanin for bilberry (16% of total anthocyanins), whereas malvidin-3-O-glucoside (A11) was the most abundant anthocyanin for bog bilberry (49% of total anthocyanins).The proportions of the different anthocyanins in bilberries were very similar for four of them: delphinidin (33%), malvidin (24%), cyanidin (22%), and petunidin (22%), and peonidin (2%) had a very low relative amount (Fig. 3a).…”

Section: S872mentioning

confidence: 99%

Phenolics and mineral content in bilberry and bog bilberry from Macedonia

Станоева

Стефова

Andonovska

et al. 2017

International Journal of Food Properties

View full text Add to dashboard Cite

Polyphenolic and mineral profiles of berries and leaves of V. myrtillus (bilberry) and V. uliginosum (bog bilberry) collected from northwestern Macedonia were examined in this study. Overall, 70 polyphenolic compounds were identified from various classes. Laricitrin-3-O-pentoside, trans-and cis-piceid, hexahydroxydiphenoyl-galoyl-glucose, and feruloylacetyl glucoside and coumaroyl glucoside of kaempferol were identified for Vaccinium genus. Bilberries had higher total phenolic content than bog bilberry. Major differences in the polyphenolic pattern of berries were found in the flavonols content, whereas leaves differed mostly in the procyanidin content. Berries and leaves of bilberry had higher K and P content compared with bog bilberry and similar contents for Ca, Mg, Mn, and Al, which were comparable to the data for other regions, whereas the measured levels of Zn, Fe, and Cu were higher than the ones reported for bilberries from other regions. ARTICLE HISTORY

show abstract

Phenolic Composition and Antioxidant Capacity of Bilberry (Vaccinium myrtillus) Leaves in Northern Europe Following Foliar Development and Along Environmental Gradients

Cited by 109 publications

References 37 publications

Antibacterial and antioxidant activities of bilberry (Vaccinium myrtillus L.) in vitro

Antibacterial and antioxidant activities of bilberry (Vaccinium myrtillus L.) in vitro

Flavan-3-ols in Norway Spruce: Biosynthesis, Accumulation, and Function in Response to Attack by the Bark Beetle-Associated Fungus Ceratocystis polonica

Phenolics and mineral content in bilberry and bog bilberry from Macedonia

Contact Info

Product

Resources

About