Phenolic Composition and Antioxidant and Antimicrobial Activities of Extracts Obtained fromCrataegus azarolusL. var.aronia(Willd.) Batt. Ovaries Calli

Abstract

Objective. Plant cell culture is an innovative technology to produce a variety of substances. Numerous plants synthesize among their secondary metabolites phenolic compounds which possess antioxidant and antimicrobial effects. Hawthorn (Crataegus) is one of these plants which has long been used in folk medicine and is widely utilized in pharmaceutical preparations mainly in neuro- and cardiosedative actions. Methods and Results. The production of polyphenol by fifty-two-week-old Crataegus azarolus var. aronia calli was studied in relation to growth variation and antioxidant and antimicrobial capacity within a subcultured period. The DPPH and ABTS+ assays were used to characterize the antioxidant actions of the callus cultures. Antimicrobial activity was tested by using disc diffusion and dilution assays for the determination of the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) values of each active extract. High TEACDPPH, TEACABTS, and antimicrobial activity was observed when maximal growth was reached. An optimum of total phenol, proanthocyanidins, flavonoid, (−)-epicatechin, procyanidin B2, chlorogenic acid, and hyperoside was produced during this period. Conclusion. Antioxidant and antimicrobial activities were strongly correlated with total phenols and total flavonoids. Crataegus azarolus var. aronia cells culture represents an important alternative source of natural antioxidants and antimicrobials.

Screening of Antioxidant and Antimicrobial Potential of Hawthorn (Creatagus Monogyna) Fruit and Leaves Extracts

“…In some studies, the hawthorn extracts showed higher antibacterial activity than did in our study (Kostic et al,2012;Bahri-sahloul et al, 2014 ). There are differences between the results of mentioned studies.…”

Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L.

“…Phenolics may have a direct contribution in the antioxidant activity [10]. The antioxidant potential in various medicinal plants has been shown mainly due to phenolic compounds [11][12][13][14][15][16]. Likewise, different species of the genus Artemisia including A. absinthium L. have shown phenolics associated antioxidant activities [4,[17][18][19].…”

Antimicrobial potential of Taraxacum officinale F.H. Wigg clonally propagated via organogenesis

“…In many studies in which BAP and IBA plant growth regulators were used in equal amounts, shoot formations have been reported as results similar to our study 23,24 . When the literature is examined, many studies on the antimicrobial potential of medicinal plants produced by plant tissue culture attract the attention 25,26 . In this study, the antimicrobial potential of the Taraxacum officinale shoots obtained through propagation have been examined.…”