Abstract:The paper presents original results concerning phenolic and mineral composition of different parts of wild chicory grown in Romania (flowers, leaves, stems and roots). Total and some individual phenols concentrations were determined using molecular absorption spectrometry (modified Folin Ciocalteu method) and HPLC-DAD. Metals concentrations were measured by FAAS. The highest total phenolic compounds concentration was founded in flowers (1531.2 mg GAE/100g d.w.), followed by leaves (1180.3 mg GAE/100g d.w.), ro… Show more
“…Previous research on the wild chicory grown in southern Romania analysed the polyphenols in different organs of the plant in methanolic extracts. Our results were higher than those reported by our colleagues (11.80 mg GAE/g d.w.) [ 41 ]. We attribute this to different sources of the vegetable material.…”
The present study analyzed the methanol extract and tincture obtained from the spontaneous Romanian Cichorium intybus species, in order to evaluate polyphenols content and some biological properties. Chromatographic and spectrophotometric methods were used for the analysis of polyphenols and the antioxidant capacity was assessed in vitro with DPPH● (2,2-diphenyl-picrylhydrazil) and FRAP (ferric-reducing antioxidant power) tests. The cardio-protective effects of Cichorii herba tincture on myocardial ischemia induced by isoprenaline and nephroprotection on renal failure induced by gentamicin were evaluated on rats. Also, aspartate aminotrasferase (AST), alanine aminotransferase (ALT), creatine kinase-MB (CK-MB) and creatinine clearance (CrCl) were measured. The antioxidant effect was evaluated by determining total oxidative stress (TOS), oxidative stress index (OSI, total antioxidant capacity (TAC), malondyaldehide (MDA), total thiols (SH) and total nitrites and nitrates (NOx). Cichoric acid was the main polyphenolic compound. The extracts had moderate in vitro antioxidant activity but the in vivo antioxidant and anti-inflammatory effects were significant and associated with myocardial and renal dysfunction improvement. The results were attributed to the content of polyphenols in the extracts, for which reason C. intybus may be considered an important raw material for pharmaceuticals formulations recommended in the prevention or treatment of heart or kidney diseases.
“…Previous research on the wild chicory grown in southern Romania analysed the polyphenols in different organs of the plant in methanolic extracts. Our results were higher than those reported by our colleagues (11.80 mg GAE/g d.w.) [ 41 ]. We attribute this to different sources of the vegetable material.…”
The present study analyzed the methanol extract and tincture obtained from the spontaneous Romanian Cichorium intybus species, in order to evaluate polyphenols content and some biological properties. Chromatographic and spectrophotometric methods were used for the analysis of polyphenols and the antioxidant capacity was assessed in vitro with DPPH● (2,2-diphenyl-picrylhydrazil) and FRAP (ferric-reducing antioxidant power) tests. The cardio-protective effects of Cichorii herba tincture on myocardial ischemia induced by isoprenaline and nephroprotection on renal failure induced by gentamicin were evaluated on rats. Also, aspartate aminotrasferase (AST), alanine aminotransferase (ALT), creatine kinase-MB (CK-MB) and creatinine clearance (CrCl) were measured. The antioxidant effect was evaluated by determining total oxidative stress (TOS), oxidative stress index (OSI, total antioxidant capacity (TAC), malondyaldehide (MDA), total thiols (SH) and total nitrites and nitrates (NOx). Cichoric acid was the main polyphenolic compound. The extracts had moderate in vitro antioxidant activity but the in vivo antioxidant and anti-inflammatory effects were significant and associated with myocardial and renal dysfunction improvement. The results were attributed to the content of polyphenols in the extracts, for which reason C. intybus may be considered an important raw material for pharmaceuticals formulations recommended in the prevention or treatment of heart or kidney diseases.
“…Some individual and total phenol concentrations of different parts of wild chicory have been determined using molecular absorption spectrometry and HPLC [ 51 ]. The flowers showed the highest total phenolic derivatives concentration (1531.2 mg Gallic acid equivalent (GAE)/100 g dry weight (d.w.)), followed by leaves (1180.3 mg GAE/100 g d.w.), roots (167.86 mg GAE/100 g d.w.) and stems (142.36 mg GAE/100 g d.w.).…”
The genus Cichorium (Asteraceae) that originates from the Mediterranean area consists of six species (Cichorium intybus, Cichorium frisee, Cichorium endivia, Cichorium grouse, Cichorium chico and Cichorium pumilum). Cichorium intybus L., commonly known as chicory, has a rich history of being known as a medicinal plant and coffee substitute. A variety of key constituents in chicory play important roles as antioxidant agents. The herb is also used as a forage plant for animals. This review highlights the bioactive composition of C. intybus L. and summarizes the antioxidant activity associated with the presence of inulin, caffeic acid derivatives, ferrulic acid, caftaric acid, chicoric acid, chlorogenic and isochlorogenic acids, dicaffeoyl tartaric acid, sugars, proteins, hydroxycoumarins, flavonoids and sesquiterpene lactones. It also covers the plant’s occurrence, agriculture improvement, natural biosynthesis, geographical distribution and waste valorization.
“…Identification and quantitative determination of the active constituents from samples macerates was done by comparing the chromatogram of standards mixture. The total phenols were estimated according to the Folin-Ciocalteau method [22][23][24][25][26][27][28].…”
Section: Identification and Quantification Of Phenolic Compounds By Hmentioning
confidence: 99%
“…The antioxidant capacity was evaluated using DPPH Radical Scavenging test. Gallic acid (GA) was used as standard to plot calibration curves and the results were expressed as equivalents (mg GAE) [14,16,[27][28][29][30][31][32]. In 25 mL calibrated flasks different volume of gallic acid solutions were added, then 5 mL DPPH 0.063% (1.268 mM) in methanol, filled up to the mark with methanol and let in the dark, to the room temperature for 45 minutes before the absorbance registration at 530 nm versus methanol.…”
The total phenols concentration in two sage macerates has been estimated by Folin-Ciocâlteau method, identified and quantified using HPLC-DAD method in order to assess the biological activity. The results for total phenols values of Folin Ciocalteau method indicate that Salvia officinalis L macerate S2, presents a higher amount of phenolic compounds than macerate S1. By HPLC-DAD method, six individual phenolic compounds were identified in sage macerates among which where cinnamic acid was found in highest concentration (652.478 mg/100g d.w. in S2 and 473.381 mg/100g d.w. in S1). The antioxidant activity of sage macerates was evaluated using DPPH Radical Scavenging test. Sage macerates exhibited high antioxidant activity, between 439.5 mg GAE /mL and 400 mg GAE /mL. Antibacterial activity of sage macerates was evaluated against 20 Gram positive and Gram negative bacterial strains isolated from clinical specimens. Both macerates showed significant but variable antibacterial activity with inhibition zones ranging from 4.97 mm (S2) to 7.28.mm (S1). The effect was stronger on Gram positive (Enterococcus, Staphyococcus) than Gram negative bacteria (Escherichia sp, Proteus sp, Klebsiella sp). Eleven metals concentrations were determined by AAS method in sage leaves; it has been found that Cd, Ni and Pb concentrations are below the detection limits.
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