Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaf extracts from ten Algerian Ficus carica L. varieties

Mahmoudi, Souhila; Khali, Mustapha; Benkhaled, Abderahim; Benamirouche, Karima; Baiti, Imen

doi:10.1016/j.apjtb.2015.12.010

Cited by 116 publications

(70 citation statements)

References 32 publications

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“…Enhanced activities of these enzymes also result in enhancement of signal transduction, preventing further pathogen spread (Kowalcyzk et al, 2004; Van Buyten and Höfte, 2013; Falcón-Rodríguez et al, 2014). PAL and POX are important components of phenylpropanoid pathway, and enhanced activities of these enzymes will lead to increased production of lignin and secondary metabolites, such as flavonoids and phenolic compounds, which have anti-microbial and anti-oxidant potential (Fraser and Chapple, 2011; Mahmoudi et al, 2016). Lignin, flavonoids, and phenolic compounds can inhibit pathogen penetration by increasing the rate of localized cell death, thereby preventing further pathogen spread from the sites of infection (Thatcher et al, 2005; Duan et al, 2014; Motallebi et al, 2015; Yoon et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Exogenous Trehalose Treatment Enhances the Activities of Defense-Related Enzymes and Triggers Resistance against Downy Mildew Disease of Pearl Millet

et al. 2016

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In recent years, diverse physiological functions of various sugars are the subject of investigations. Their roles in signal transduction in plant responses to adverse biotic and abiotic stress conditions have become apparent, and growing scientific evidence has indicated that disaccharides like sucrose and trehalose mediate plant defense responses in similar way as those induced by elicitors against the pathogens. Trehalose is a well-known metabolic osmoregulator, stress-protectant and non-reducing disaccharide existing in a variety of organisms, including fungi, bacteria, and plants. Commercially procured trehalose was applied to seeds of susceptible pearl millet (Pennisetum glaucum) cultivar “HB3,” and tested for its ability to reduce downy mildew disease incidence by induction of resistance. Seed treatment with trehalose at 200 mM for 9 h recorded 70.25% downy mildew disease protection, followed by those with 100 and 50 mM trehalose which offered 64.35 and 52.55% defense, respectively, under greenhouse conditions. Furthermore, under field conditions treatment with 200 mM trehalose for 9 h recorded 67.25% downy mildew disease protection, and reduced the disease severity to 32.75% when compared with untreated control which displayed 90% of disease severity. Trehalose did not affect either sporangial formation or zoospore release from sporangia, indicating that the reduction in disease incidence was not due to direct inhibition but rather through induction of resistance responses in the host. Additionally, trehalose was shown to enhance the levels of polyphenol oxidase, phenylalanine ammonia lyase, and peroxidase, which are known as markers of both biotic and abiotic stress responses. Our study shows that osmoregulators like trehalose could be used to protect plants against pathogen attacks by seed treatment, thus offering dual benefits of biotic and abiotic stress tolerance.

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Section: Discussionmentioning

confidence: 99%

Exogenous Trehalose Treatment Enhances the Activities of Defense-Related Enzymes and Triggers Resistance against Downy Mildew Disease of Pearl Millet

et al. 2016

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“…Petri dishes were then incubated during 24 h at 37ºC for bacterial strains. Antibacterial activity was evaluated by measuring the inhibition zone (mm) against the studied microorganisms, including disc diameter [10].…”

Section: Determining Total Anthocyanin Contentmentioning

confidence: 99%

Chemical Composition, Antioxidant and Antibacterial Activity of Tunisian Date Palm Seed

Metoui¹,

Essid²,

Bouzoumita³

et al. 2018

Pol. J. Environ. Stud.

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Date palm (Phoenix dactylifera L.) is an important crop in arid and semiarid regions. Date palm fruits constitute the principal source of remuneration and the basis of the economy for people living in the Tunisian Sahara. Phoenix dactylifera is a member of the family Arecaceae, also known as Palmaceae. It is well known that the average weight of date seeds is about 10-15% of date weight [1]. The fruit of the date palm is composed of a fleshy pericarp and seed. The edible part of date fruit is fleshy mesocarp, which exists in-between the epicarp and hard endocarp. Mesocarp of date fruit has more than 80% sugars and the remaining 20% has dietetic fibers, protein, Pol. J. Environ. Stud. Vol. 28, No. 1 (2019), 267-274 AbstractThe seeds of 11 common dates (phoenix dactylifera L.) cultivars from the coastal oasis of Gabés, and the noble variety Deglet Nour from the oasis of Kébéli were evaluated for their chemical composition. Polyphenol, flavonoid, anthocyanin, antioxidant, and antimicrobial activity was recorded against 5 pathogen bacteria (Escherichia coli, staphylococcus aureus, staphylococcus epidermis, and Salmonella Typhinurium). The sugar content ranged between 1.20 g/100 gMS ( Bouhattam) and 3.80 g/100 gMS ( Khadhouri). The total phenolic content oscillates between 5.224 g/100 gMS (Baht) and 9.532 g/100 gMS (Khadhouri). Khadhouri also had the highest total flavonoids content (3.82 g/100 gMS). The higher antioxidant activity was found on the Khadhouri cultivar (55.47% of DPPH radical scavenging activity). It has been found that a linear relationship (p<0.01) exists between total phenolic content and inhibition percentage. Antibacterial avtivities clearly show that all the extracts showed antibacterial activity except Enterococcus feacalis. PCA revealed that the first two principal components (PC1 and PC2) accounted for 54.23% of the total variance within the data set. These two principal components separated the studied cultivars into four different groups. This by-product of date processing industries could be regarded as an excellent source of food ingredients with interesting technological functionality that could also be used in medicinal preparation as an important source of oil.

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“…Saponin leads to leakage of proteins and some enzymes from the microbial cell and thus responsible for antimicrobial activity [28]. A previous study has shown that flavonoids and phenolic components present in the plant extract have potential antimicrobial activity [29]. Hence, we could hypothesize that the antimicrobial activity of Rue plant might be due to the presence of the above-mentioned phytoconstituents in the extract.…”

Section: Analysis Of Gc-ms Profilementioning

confidence: 97%

Phytochemical Investigation, Gc-MS Profile and Antimicrobial Activity of a Medicinal Plant Ruta Graveolens L. From Ethiopia

Azalework

Sahabjada

Jafri

et al. 2017

Int J Pharm Pharm Sci

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Objective: This study was designed to screen the phytochemicals present in various solvents extracts of Ruta graveolens (Rue) and furthermore to investigate their antimicrobial activity. Methods:The leaves, stems and seeds of Rue were extracted using four different solvents viz. ethanolic, methanolic, chloroform, and aqueous of varying polarity. The phytochemical screening was carried out qualitatively and Gas Chromatography-Mass Spectroscopy (GC-MS) analysis was performed to identify major phytoconstituents present in the methanolic leaf extract. The antimicrobial effect of extracts was evaluated against six microbial strains namely Bacillus subtillis, Escherichia coli, Proteus vulgaris, Candida albicans, Candida tropicalis and Micrococcus luteus with disc diffusion method.Results: Phytochemical analysis revealed the presence of various secondary metabolites such as flavonoids, alkaloids, terpenoids, saponins and carotenoid. The methanolic leaf extract showed the presence of both tannin and phenolic contents in the higher amount, whereas aqueous extract displayed in the least amount. GC-MS analysis of methanolic leaf extract revealed the presence of approximately 26 phytochemical constituents. The antimicrobial assay revealed that B. subtilis showed a high zone of inhibition (20 mm) at 200 mg/ml of methanolic extract. However, E. coli and C. tropicalis did not show any zone of inhibition against each solvent extract. Conclusion:In conclusion, secondary metabolites present in the extracts have biological activities which warrant further to evaluate in vivo pharmacological studies.

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Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaf extracts from ten Algerian Ficus carica L. varieties

Cited by 116 publications

References 32 publications

Exogenous Trehalose Treatment Enhances the Activities of Defense-Related Enzymes and Triggers Resistance against Downy Mildew Disease of Pearl Millet

Exogenous Trehalose Treatment Enhances the Activities of Defense-Related Enzymes and Triggers Resistance against Downy Mildew Disease of Pearl Millet

Chemical Composition, Antioxidant and Antibacterial Activity of Tunisian Date Palm Seed

Phytochemical Investigation, Gc-MS Profile and Antimicrobial Activity of a Medicinal Plant Ruta Graveolens L. From Ethiopia

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