Phenolic and acetylenic metabolites from Artemisia assoana

Martínez, Vladimir; Barberá, Óscar; Sánchez-Parareda, J.; Marco, J. Alberto

doi:10.1016/s0031-9422(00)83891-1

Cited by 103 publications

(62 citation statements)

References 19 publications

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“…By screening 110 commercial essential oils from different plants using a microbioassay technique, Yoshinari et al (2008) found a novel biological activity from M. recutita that completely inhibited AFG 1 production in A. parasiticus NRRL2999 at a concentration of 100 µg/ml. The components associated with the activity were identified as (E)-and (Z)-spiroethers (Martinez et al 1987). Both compounds inhibited AFG 1 production with IC 50 values of 2.8 and 20.6 µM without affecting fungal growth.…”

Section: Spiroethers From Matricaria Recutitamentioning

confidence: 98%

Aflatoxins: Mechanisms of Inhibition by Antagonistic Plants and Microorganisms

Razzaghi‐Abyaneh¹,

Shams-Ghahfarokhi²,

Chang³

2011

Aflatoxins - Biochemistry and Molecular Biology

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Section: Spiroethers From Matricaria Recutitamentioning

confidence: 98%

Aflatoxins: Mechanisms of Inhibition by Antagonistic Plants and Microorganisms

Razzaghi‐Abyaneh¹,

Shams-Ghahfarokhi²,

Chang³

2011

Aflatoxins - Biochemistry and Molecular Biology

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“…Many anticancer agents arrest the cell cycle at the G 0 /G 1 , S, and G 2 /M phase and then induce apoptosis (Martínez et al, 1987;Murray, 2004;Torres & Horwitz, 1998). Hence, the effect of E. hirta on the cell cycle was determined to identify the mechanism of action of E. hirta inhibiting MCF-7 cell growth.…”

Section: Cell Cyclementioning

confidence: 99%

Evaluation of the cytotoxicity, cell-cycle arrest, and apoptotic induction byEuphorbia hirtain MCF-7 breast cancer cells

Kwan

Saito

Demirtaş

et al. 2015

Pharmaceutical Biology

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Context: Euphorbia hirta L. (Euphorbiaceae) has been used as a folk remedy in Southeast Asia for the treatment of various ailments. Objective: The current study evaluates the cytotoxicity, cell-cycle arrest, and apoptotic induction by E. hirta in MCF-7 breast cancer cells. Materials and methods: Cytotoxic activity of methanol extract of whole part of E. hirta was determined by the MTT assay at various concentrations ranging from 1.96 to 250.00 mg/mL in MCF-7 cells. Cell morphology was assessed by light and fluorescence microscopy. Apoptosis and cell-cycle distribution were determined by annexin V staining and flow cytometry. DNA fragmentation, caspase activity, and reactive oxygen species (ROS) assays were performed using the commercially available kits. To identify the cytotoxic fraction, E. hirta extract was subjected to bioassay-guided fractionation. Results: Euphorbia hirta exhibited significant inhibition of the survival of MCF-7 cells and the half inhibitory concentration (IC 50 ) values was 25.26 mg/mL at 24 h. Microscopic studies showed that E. hirta-treated cells exhibited marked morphological features characteristic of apoptosis. Euphorbia hirta extract also had an ignorable influence on the LDH leakage and generating intracellular ROS. The flow cytometry study confirmed that E. hirta extract induced apoptosis in MCF-7 cells. Euphorbia hirta also resulted in DNA fragmentation in MCF-7 cells. Moreover, E. hirta treatment resulted in the accumulation of cells at the S and G 2 /M phases as well as apoptosis. The caspase activity study revealed that E. hirta extract induced apoptosis through the caspase-3-independent pathway by the activation of caspase-2, 6, 8, and 9. Euphorbia hirta hexane fraction, namely HFsub4 fraction, demonstrated highest activity among all the fractions tested with an IC 50 value of 10.01 mg/mL at 24 h. Discussion and conclusion: This study revealed that E. hirta induced apoptotic cell death and suggests that E. hirta could be used as an apoptosis-inducing anticancer agent for breast cancer treatment with further detailed studies.

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“…The present paper describes the isolation and structure elucidation of two new bisabolane-type sesquiterpenes (3, 4) along with five known flavonoids (5-9), three known phenylethanoid glycosides (10)(11)(12) and two known iridoid glucosides (13,14), as well as the anioxidative activity of eight phenolic compounds (5)(6)(7)(8)(9)(10)(11)(12).…”

mentioning

confidence: 99%

“…The MeOH extract of the aerial parts of L. dulcis was successively subjected to Diaion HP20, silica gel, Sephadex LH20 and Chromatorex ODS column chromatographies as well as HPLC on ODS to afford twelve compounds (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14).…”

mentioning

confidence: 99%

“…Compounds 5-14 were identified as cirsimaritin (5), 5) salvigenin (6), 6) eupatorin (7), 7) 5-hydroxy-6,7,3Ј,4Ј-tetramethoxyflavone (8), 8) 5,3Ј-dihydroxy-6,7,4Ј,5Ј-tetramethoxyflavone (9), 9) decaffeoylverbascoside (10), 10) acteoside (11), 11) isoacteoside (12), 12) 8-epiloganin (13) 13) and lamiide (14), 14) respectively, based on their physical and spectral data. Compounds 3 and 4 were obtained as a colorless oil.…”

mentioning

confidence: 99%

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