Phenolic acid content, antioxidant and cytotoxic activities of four Kalanchoë species

Abstract

Phenolic acid composition, antioxidant, and cytotoxic activities in leaves of four (Crassulaceae) species were evaluated. Determination of phenolic acid contents were conducted by an optimized LC-ESI-MS/MS method. The results show that Raym.-Hamet & H. Perrier (using ASE extraction) and (Lam.) Pers. contain the highest amounts of phenolic acids, while Engl. the lowest ones. Among phenolic acids ferulic, caffeic and protocatechuic acids were occurring in the highest quantities in the analysed species. The greatest amounts of ferulic and protocatechuic acids were found in and Moreover, the antiradical and cytotoxic activities of extracts were investigated. All tested extracts possessed antioxidant activity. The obtained IC values (μg/mL) ranged from 49.9 μg/mL to 1410 μg/mL, indicating a large variation of the activity of the analysed extracts. Cytotoxicity assays revealed dose-dependent effects in the cells lines tested. Only extract showed a high cytotoxicity against the H-9 human T cell line. Other extracts (, ,) showed more pronounced cytotoxicity towards J45.01 cells (human acute lymphoblastic leukaemia T cells). The present study demonstrated that extracts have significant antioxidant and cytotoxic effects. This suggests that these species can be used as new sources of natural antioxidants and potential anticancer compounds.

Identification of Flavonoids and Bufadienolides and Cytotoxic Effects of Kalanchoe daigremontiana Extracts on Human Cancer Cell Lines

“…Kalanchoe daigremontiana has anti-inflammatory, antimicrobial, antioxidant, and cytotoxic activities [9][10][11][12]. However, the effect of extracts of this plant on cancer cells was tested only on human lymphoblastic leukemia T cells (H9 and J45 cell lines) with estimation of inhibitory concentration values [11].…”

“…The different activities of K. daigremontiana depend on its phytochemical composition, and its analysis has shown that flavonoids, phenolic acids, and bufadienolides are the main metabolites [8,11,[13][14][15]. To date, only 4 flavonoid compounds of K. daigremontiana have been reported in the literature [8,16].…”

Assessment of the antioxidant properties of the most common coffee brews available in the local markets of the western region of Saudi Arabia

“…In plants, most of the antioxidant potential is due to the redox properties of phenolic compounds, which act as hydrogen donors, singlet oxygen quenchers and reducing agents (Kasote et al, 2015). Through various mechanisms, antioxidant activity of polyphenols may act as reducing, chelating and scavenging agent; act as cofactors of enzymes catalyzing oxidative reactions, terminate radical chain reactions, inhibit oxidases, and stabilize free radicals (Bogucka-Kocka et al, 2016). Maillard reaction is responsible for the generation of compounds called melanoidins, brown pigments in roasted coffee, from reducing amino acids and proteins or sugars during food processing and preservation (Wang et al, 2011;Karamac et al, 2005).…”

Antioxidant and xanthine oxidase inhibitory properties and LC-MS/MS identification of compoundsof ethanolic extract from Mulberry leaves

“…org/10.17306/J. AFS.2018.0587 anticancer and anti-microbial activities and can act as modulators of the activities of enzymes such as XO (Bogucka-Kocka et al, 2016;Danihelová et al, 2012). Moreover, mulberry leaves consist of rutin, isoquercetin, astragalin, which have been shown to be effective antioxidants and XO inhibitors (Chiang et al, 1994;Masuoka et al, 2012).…”