Phenol-Rich Compounds Sweet Gel: A Statistically More Effective Antibiotic than Cloxacillin Against Pseudomonas Aeruginosa

Abstract

Objectives:The purpose of this study was to obtain a natural antibiotic from Phenol-rich compounds; for the dressing and the treatment of chronic wounds.Methods:The Phenol-rich compound sweet gel was prepared by blending four natural herbal extracts, Acacia catechu (L.F.), Momia (Shilajit), Castanea sativa, and Ephedra sinica stapf, with combination of a sweet gel medium, including honey, maple saps, Phoenix dactylifera L. (date), pomegranate extract and Azadirachta indica gum as a stabilizer. The combinations were screened by using a well-diffusion assay with cloxacillin as a control. Pseudomonas spp. was tested with our novel antimicrobial compound. The zones of inhibition in agar culture were measured for each individual component and for the compound, and the results were compared with those of the control group which had been treated with cloxacillin. Data were expressed as means ± standard deviations. Quantitative analyses were performed using the paired t-test.Results:The antibiotic effect of the Phenol-rich compound sweet gel was statistically shown to be more significant than that of cloxacillin against Pseudomonas aeruginosa (P < 0.05).Conclusion:Our novel approach to fighting the antibiotic resistance of Pseudomonas proved to be successful. The Phenol-rich compound sweet gel was found to be suitable for use as an alternative medicine and bioactive dressing material, for the treatment of patients with various types of wounds, including burns, venous leg ulcers, ulcers of various etiologies, leg ulcers on the feet of diabetic, unhealed graft sampling sites, abscesses, boils, surgical wounds, necrotic process, post-operative and neonatal wound infection, and should be considered as an alternative to the usual methods of cure.

<p>Honey Combination Therapies for Skin and Wound Infections: A Systematic Review of the Literature</p>

“…Even more complex mixtures of natural agents have been tested. For example, Dashtdar et al 2016 42 prepared a gel composed of herbal extracts of Acacia catechu, Castanea sativa, Ephedra sinica and Momia combined with honey (25%), maple saps, Phoenix dactylifera (date), pomegranate extract and Azadirachta indica gum. This gel had a higher antibacterial activity in an agar well diffusion assay than the antibiotic cloxacillin or honey alone against P. aeruginosa .…”

“… 41 Iranian honey (25%) from Fasa, Iran Gel composed of herbal extracts of Acacia catechu, Castanea sativa, Ephedra sinica and Momia combined with honey (25%), maple saps, Phoenix dactylifera (date), pomegranate extract and Azadirachta indica gum Agar well diffusion assay P. aeruginosa Zone of inhibition induced by the herbal gel formulation was greater than honey alone (35.1 mm vs 13.1 mm respectively). 42 Slovakian honeys (sunflower, acacia and honeydew) UMF 15+ manuka honey (Natures Nectar, UK) Vitamin C (Sub-inhibitory concentrations) MIC assay Biofilm wound model Planktonic bacteria including P. aeruginosa and S. aureus . Multispecies biofilms ( P. aeruginosa, S. aureus, S. agalactiae, E. faecalis ) Supplementation of honey with vitamin C reduced the MIC of all honey types tested against planktonic P. aeruginosa .…”

The Effect of Neem (Azadirachta indica) Gum Administration to Malondialdehyde Level of Wistar Rats’ Kidney Induced by Diazinon

“…Gum mimba (Azadirachta indica) adalah salah satu bagian tanaman yang berpotensi sebagai agen antioksidan karena memiliki kandungan polisakarida yang tinggi meliputi glukosa, arabinosa, fukosa, manosa, xilosa, dan asam uronat (Samrot et al, 2020). Gum mimba yang merupakan eksudat alami dari kulit batang mimba ini memiliki rasa manis dan mudah dicerna sehingga dijadikan sebagai pelapis obat dalam bidang farmasi (Dashtdar et al, 2016;Ketkar et al, 2005;Kulkarni et al, 2013). Dibandingkan dengan beberapa bagian tanaman mimba lainnya seperti bagian daun dan biji, gum mimba memiliki kemampuan antioksidan yang lebih tinggi jika ditinjau dari perhitungan EC50 dalam menghambat radikal bebas DPPH (Abdulkadir et al, 2017;Malviya et al, 2017;Sithisarn & Gritsanapan, 2005).…”