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PART I. METHODS OF PURE CULTURE STUDY. REVISED The 1917 report of this committee contained a description of the methods recommended to be used with the Descriptive Chart. Copies of the report have been on sale by the Society and the first pdition is now practically exhausted. Opportunity is therefore Jtaken to revise the report, printing it in the JOURNAL OF BAC-TEIOLOGY to cal it to the attention of the members of the Society, the reprints to be placed on sale as before. As soon as these reprints are available, one copy will be furnished with every order for Descriptive Charts, and additional copies may be obtained for 15 cents apiece postpaid, or if five or more copies are ordered, for 10 cents each, transportation not postpaid. Orders for these reports, as well as for the Descriptive Charts, are to be sent to the chairman of the committee (address Geneva, N. Y.). Methods are given in this report for all the determinations listed on the 1917 chart (recommended for instruction) but methods have not yet been prepared for all the determinations called for by the older chart. As the chart designed for instruction is now in most general use, it has seemed wisest to give further attention to these few methods rather than to do work on some of the other, less frequently used, determinations. The methods given here are not to be considered official. They are merely the best that have come to the attention of the committee at the present time. Criticisms and suggestions are at all times welcome, in order that future editions of this report may be brought up-to-date.
PART I. METHODS OF PURE CULTURE STUDY. REVISED The 1917 report of this committee contained a description of the methods recommended to be used with the Descriptive Chart. Copies of the report have been on sale by the Society and the first pdition is now practically exhausted. Opportunity is therefore Jtaken to revise the report, printing it in the JOURNAL OF BAC-TEIOLOGY to cal it to the attention of the members of the Society, the reprints to be placed on sale as before. As soon as these reprints are available, one copy will be furnished with every order for Descriptive Charts, and additional copies may be obtained for 15 cents apiece postpaid, or if five or more copies are ordered, for 10 cents each, transportation not postpaid. Orders for these reports, as well as for the Descriptive Charts, are to be sent to the chairman of the committee (address Geneva, N. Y.). Methods are given in this report for all the determinations listed on the 1917 chart (recommended for instruction) but methods have not yet been prepared for all the determinations called for by the older chart. As the chart designed for instruction is now in most general use, it has seemed wisest to give further attention to these few methods rather than to do work on some of the other, less frequently used, determinations. The methods given here are not to be considered official. They are merely the best that have come to the attention of the committee at the present time. Criticisms and suggestions are at all times welcome, in order that future editions of this report may be brought up-to-date.
The question of just how far environmental changes will affect the cultural characters of a species or a single strain of a species is one that needs more study. A review of the literature shows considerable contradictory evidence resulting from the experiments of different investigators. In an attempt to work out the relation existing between the growth and fermentative powers of bacteria, Herter (1909) and Jacoby (1917) came to opposite conclusions. The former concludes from his work that the fermentative power of species may be greatly impaired or entirely lost without any appreciable effect upon their growth; whereas Jacoby contends that growth and fermentative ability run a fairly parallel course. Pacini (1918), working on the vitamine content of bacteria obtained an excellent growth on Uschinsky's medium with the Anderson strain of Bact. typhosum, while Damon (1921), working on the same problem and using the same medium, was unable to obtain any growth with the typhoid strain which he used. Kirstein (1904) found that, when cultivated on synthetic medium, the typhoid bacillus became spontaneously agglutinable, while Frankel and Barker (1919) reported no change in the agglutinability of the typhoid bacterium cultivated on various synthetic media. Joos (1902), Joachin (1904), and Scheller (1905), (1906) have come to the conclusion that heating the typhoid bacillus alters its antigenic and 1 Thesis submitted to the Faculty of Brown University in partial fulfillment of the requirements for the degree of Doctor of Philosophy.
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