Phenol metabolism in downy mildew resistant and susceptible genotypes of pearl millet

Mahatma, M. K.; Bhatnagar, Rakesh; Mittal, Gaurav; Mahatma, Lalit

doi:10.1080/03235400903266404

Cited by 14 publications

(8 citation statements)

References 23 publications

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“…5 ). A similar kind of trend was observed in pearl millet plants during downy mildew infection [ 31 ]. Few sugars such as glucopyranose and xylulose contents were higher in control and IS-1 stages, while 3-hydroxy-glutaric acid was higher at control, IS-1 and IS-2 stages of GPBD-4.…”

Section: Resultssupporting

confidence: 77%

Metabolic profiles of peanut (Arachis hypogaea L.) in response to Puccinia arachidis fungal infection

Rathod,

Tomar

et al. 2023

BMC Genomics

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Background Puccinia arachidis fungus causes rust disease in the peanut plants (Arachis hypogaea L.), which leads to high yield loss. Metabolomic profiling of Arachis hypogaea was performed to identify the pathogen-induced production of metabolites involved in the defense mechanism of peanut plants. In this study, two peanut genotypes, one susceptible (JL-24) and one resistant (GPBD-4) were inoculated with Puccinia arachidis fungal pathogen. The metabolic response was assessed at the control stage (0 day without inoculation), 2 DAI (Day after inoculation), 4 DAI and 6 DAI by Gas Chromatography-Mass Spectrometry (GC-MS). Results About 61 metabolites were identified by NIST library, comprising sugars, phenols, fatty acids, carboxylic acids and sugar alcohols. Sugars and fatty acids were predominant in leaf extracts compared to other metabolites. Concentration of different metabolites such as salicylic acid, mannitol, flavonoid, 9,12-octadecadienoic acid, linolenic acid and glucopyranoside were higher in resistant genotype than in susceptible genotype during infection. Systemic acquired resistance (SAR) and hypersensitive reaction (HR) components such as oxalic acid was elevated in resistant genotype during pathogen infection. Partial least square-discriminant analysis (PLS-DA) was applied to GC-MS data for revealing metabolites profile between resistant and susceptible genotype during infection. Conclusion The phenol content and oxidative enzyme activity i.e. catalase, peroxidase and polyphenol oxidase were found to be very high at 4 DAI in resistant genotype (p-value < 0.01). This metabolic approach provides information about bioactive plant metabolites and their application in crop protection and marker-assisted plant breeding.

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Section: Resultssupporting

confidence: 77%

Metabolic profiles of peanut (Arachis hypogaea L.) in response to Puccinia arachidis fungal infection

Rathod,

Tomar

et al. 2023

BMC Genomics

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“…Similar observations were reported by Mahatma et al, (2011). Mahatma et al, (2011) observed that genotypes reported increased content of phenolics but without conferring any resistance.…”

Section: Figure 1:-comparisons Of Secondary Metabolites Absorbance Cosupporting

confidence: 79%

“…Similar observations were reported by Mahatma et al, (2011). Mahatma et al, (2011) observed that genotypes reported increased content of phenolics but without conferring any resistance. This could be attributed to the fact reported by Wu et al, (2015) that plant phenolics were not toxic to insects unless prophenoloxidase genes are lost or the levels of phenolics exceed the catalytic activity of the gut prophenoloxidases.…”

Section: Figure 1:-comparisons Of Secondary Metabolites Absorbance Cosupporting

confidence: 79%

Biochemicals Associated With Callosobruchus Chinensis Resistance in Soybean.

MsiskaU¹,

ijar²,

MieshoB³

et al. 2018

IJAR

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Soybean is a very important crop worldwide because of its 40% protein and 20% oil content. However, soybean suffers damage from bruchid (Callosobruchus chinensis) during storage. Understanding of factor(s) contributing to bruchid resistance is useful for development of soybean cultivars with genetic resistance to bruchid damage. Biochemicals associated with resistance to Callosobruchus chinensis were investigated on eight soybean genotypes with varying levels of resistance. Significant differences (P<0.05) were observed amongst genotypes with regards to antinutritional factors particularly total antioxidants (TAOX), tannins, peroxidase (POD), and flavonoids. There were no significant differences among genotypes with regards to nutritional factors particularly proteins, starch, lipid peroxidation, and reducing sugars. A resistant genotype AVRDC G8527 had the highest concentration of total antioxidants (1.98 AU min -1 mg -1 ) and tannins (1.85mg TAE 100g -1 ) followed by Maksoy 3N while the least was in a very susceptible genotype AGS 292 (TAOX=0.39 AU min -1 mg -1 , Tannin=0.296 mg TAE 100g -1 ). AGS 292 had the highest concentration of flavonoids (31.22 mg QE 100g -1 ) while AVRDC G8527 had the least (5.13 mg QE 100g -1 ). Peroxidase activity was highest in AVRDC G89 (0.27AU min -1 mg -1 ) while AGS 292 had the least (0.07AU min -1 mg -1 ). From the correlation analysis, a significant strong negative relationship between flavonoids and tannin (r=-0.73 ** ) and TAOX (r=-0.71 ** ) but a positive non significant relationship with phenolic (r=0.22) and alkaloids (r=0.37) was recorded. Peroxidase activity had a significant relationships with median development period (r=0.69 * ) indicating that increased peroxidase activity resulted into increased seed resistance through prolonged insect development period. There was a strong relationship between tannins and total antioxidants (r=0.99 ** ). These results indicate that secondary metabolites; peroxidase, tannin, and TAOX were biochemicals associated with higher resistance to C. chinensis in soybean while flavonoids were associated with higher susceptibility.Copy Right, IJAR, 2018,. All rights reserved. Corresponding Author:-Msiska U. M. ISSN: 2320-5407Int. J. Adv. Res. 6(5), 292-305is an annual legume that belongs to the legume family Fabaceae and is classed as an oil crop (Singh et al., 2007). Soybean is one of the five oldest cultivated crops and was utilized by the Chinese as a source of food before 2500 BC (Gibson et al., 2005). Nigeria is the largest producer of soybean in sub-Saharan Africa (SSA), followed by South Africa (Tefera, 2015). Commercial soybean production on large farms takes place in Zambia, Zimbabwe and South Africa (Tefera, 2015). However, it is mostly cultivated by small-scale farmers in other parts of Africa where it is planted as a minor food crop among sorghum, maize, or cassava. In Africa, Uganda is the second largest consumer of soybean following Nigeria (Murithi et al., 2016). Soybean is among the major industrial, feed and food crops gro...

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“…Enzyme activity related to phenylpropanoid pathway viz. Phenylalanine ammonia lyase (PAL) activity was measured by the method as described by Mahatma, et al, (2011) [23] . Cinnamic acid-4-hydroxylase (C4H) and p-Coumaric acid-3hydroxylase (C3H) activity was measured by the method as described by Padhiar & Sharma, (2008) [30] .…”

Section: Biochemical Parametersmentioning

confidence: 99%

Dissection of phenylpropanoid pathway during salt stress in cotton (<em>Gossypium hirsutum</em> L.)

Aadil¹,

Jha²,

Parekh³

et al. 2020

Int. J. Chem. Stud.

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Salt stress is an abiotic stress in cotton (G. hirsutum L.) that affects evenly yield. The present investigation carried out to study the significance of integrative biochemical and molecular approaches against salt stress in cotton. Four different verities, two salt tolerant (G.Cot.-16, GISV.218) and two susceptible (G. Cot.10, G.Cot.-100) were used screened at different salinity levels viz., 0.8 dS/m, 3 dS/m, 7 dS/m and 10 dS/m. Bio-chemical and molecular parameters analysed at seedling (15 DAS) and squaring stage (45 DAS). Biochemical traits like Protein content, total phenol content and Proline content was found to be increased with increasing salinity levels. Enzyme activity related to phenylpropanoid pathway (PAL, C4H and C3H) was found to be also increased with the advancement in salt stress, hence it implies possible role of these enzymes in imparting tolerance to salt stress in cotton. Reactive oxygen species (ROS) scavenging enzymes such as SOD, POD, CAT and PPO recorded increase in their activity at both seedling and squaring stages with increasing salinity levels. Expression analysis of key genes of phenylpropanoid pathway viz. PAL, C4H (C4H1 and C4H2) revealed maximum fold expression of PAL and C4H1 in the leaves, whereas least fold expression was observed for C4H2. Thus, it may be concluded that phenylpropanoid pathway play significant role in imparting tolerance against salt stress in cotton.

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Phenol metabolism in downy mildew resistant and susceptible genotypes of pearl millet

Cited by 14 publications

References 23 publications

Metabolic profiles of peanut (Arachis hypogaea L.) in response to Puccinia arachidis fungal infection

Metabolic profiles of peanut (Arachis hypogaea L.) in response to Puccinia arachidis fungal infection

Biochemicals Associated With Callosobruchus Chinensis Resistance in Soybean.

Dissection of phenylpropanoid pathway during salt stress in cotton (<em>Gossypium hirsutum</em> L.)

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