Phenol degradation by isolated bacterial strains: kinetics study and application in coking wastewater treatment

Successful bioremediation of a phenol-contaminated environment requires application of those microbial strains that have acquired phenol tolerance and phenol-degrading abilities. A newly isolated strain B9 of Acinetobacter sp. was adapted to a high phenol concentration by growing sequentially from low-to highstrength phenol. The acclimatised strain was able to grow and completely degrade up to 14 mM of phenol in 136 h. The degradation rates were found to increase with an increase in the phenol concentration from 2.0 to 7.5 mM. The strain preferred neutral to alkaline pH range for growth and phenol degradation, with the optimum being pH 8.0. The optimum temperature for phenol degradation was found to be in the range of 30-35°C. Transmission electron micrographs showed a disorganised and convoluted cell membrane in the case of phenol-stressed cells, showing a major effect of phenol on the membrane. Enzymatic and gas chromatography-mass spectrometry studies show the presence of an ortho-cleavage pathway for phenol degradation. Efficient phenol degradation was observed even in the presence of pyridine and heavy metals as co-toxicants showing the potential of strain in bioremediation of industrial wastes. Application of strain B9 to real tannery wastewater showed 100% removal of initial 0.5 mM phenol within 48 h of treatment.

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“…[15,26] Thus, the dephenolising ability of the strain was tested in the co-presence of these toxicants. The Acinetobacter sp.…”

Section: Phenol Biodegradation In the Presence Of Pyridine And Heavy mentioning

confidence: 99%

Assessment of phenol-degrading ability ofAcinetobactersp. B9 for its application in bioremediation of phenol-contaminated industrial effluents

Bhattacharya

Gupta

Kaur

et al. 2015

Chemistry and Ecology

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“…Furthermore, the addition of phenol‐degrading bacteria to a biological contact oxidation reactor improved total phenol removal from 66% to 80% . Removal of phenol from coke wastewater was considered by Zhu et al . who isolated two different strains of Pseudomonas (sp.PCT01 and PTS02).…”

Section: Introductionmentioning

confidence: 99%

Enhancing the removal of pollutants from coke wastewater by bioaugmentation: A scoping study

Raper

Stephenson

Simoes

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Bioaugmentation and biostimulation were investigated for their ability to improve the removal of thiocyanate (SCN–), polycyclic aromatic hydrocarbons (PAHs), phenol and trace metals in coke wastewater. Additionally, the ability of the microorganisms supplemented with the bioaugmentation product to survive in a simulated river water discharge was evaluated. RESULTS A commercially available bioaugmentation product composed mainly of Bacillus sp. was mixed with activated sludge biomass. A dose of 0.5 g L–1 increased the removal of Σ6PAHs (sum of fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, indeno[1,2,3‐cd]pyrene and benzo[g,h,i]perylene) by 51% and reduced SCN– below 4 mg L–1 enabling compliance with the EU Industrial Emissions Directive (IED). Biostimulation (supplementing micronutrients and alkalinity) allowed compliance for both SCN– and phenol (<0.5 mg L–1). Bacillus sp. accounted for 4.4% of the microbial population after 25 h (1.5 g L–1 dose) which declined to 0.06% after exposure to river water (24 h). Exposure of the activated sludge biomass to river water resulted in a 98.6% decline in viable cell counts. CONCLUSION In order to comply with the IED, bioaugmentation and biostimulation are recommended for the treatment of coke wastewater to enable an effluent Σ6PAHs of 6.6 µg L–1, 0.3 mg L–1 phenol and 1.2 mg L–1 SCN–. Such techniques are not anticipated to impact on downstream river water quality. © 2018 Society of Chemical Industry

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“…which are difficult to degrade. Many of these compounds are highly toxic, refractory and carcinogenic, and may cause serious pollution of the environment and be a direct threat to human health …”

Section: Introductionmentioning

confidence: 99%

“…The characterization and coagulation behaviour of these coagulants were also studied. But investigations of the synthesis and optimization of PAFSiC from coal gangue are limited . To the best of our knowledge, the fabrication of a composite coagulant of PAFSiC customized for the tertiary‐treatment of coking wastewater has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and optimization of a novel poly‐silicic metal coagulant from coal gangue for tertiary‐treatment of coking wastewater

Liu

Wang

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND Bio‐treated coking wastewater is difficult to treat further, mainly due to the bio‐refractory organic content. Coal gangue is the largest solid waste in China and it contains large amounts of aluminum, iron and silicon. This study attempts to recover the waste in coal gangue as a novel poly‐silicic metal coagulant for bio‐treated coking wastewater treatment. RESULTS The poly‐silicic metal coagulant was synthesized from coal gangue under different conditions. Fourier transform infrared spectroscopy, X‐ray diffraction and scanning electron microscopy confirmed that both amorphous and crystalline phases were present in the samples and new chemical bonds such as Si–O–Al and Si–O–Fe were formed. The results show that the composite coagulant was effective for organic removal from the bio‐treated coking wastewater and the optimal Al/Fe/Si molar ratios were Al/Fe = 3 and (Al + Fe)/Si = 13. The removal of organics exhibits a parabolic curve as the coagulant dosage increases. However, the optimal dosage (125 mg L‐1) for organic removal was not equal to the isoelectric point (100 mg L‐1). Furthermore, SEM images showed that the flocs start to disintegrate at a dosage of 150 mg L‐1, leading to deteriorating sludge settling. CONCLUSIONS It is feasible to fabricate poly‐silicic metal coagulants for coking wastewater treatment by using coal gangue as the raw material. The removal of the bio‐refractory organics by the composite coagulant was attributed to the combined effects of charge neutralization, bridge adsorption and the net capturing. © 2017 Society of Chemical Industry

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Phenol degradation by isolated bacterial strains: kinetics study and application in coking wastewater treatment

Cited by 20 publications

References 33 publications

Assessment of phenol-degrading ability ofAcinetobactersp. B9 for its application in bioremediation of phenol-contaminated industrial effluents

Assessment of phenol-degrading ability ofAcinetobactersp. B9 for its application in bioremediation of phenol-contaminated industrial effluents

Enhancing the removal of pollutants from coke wastewater by bioaugmentation: A scoping study

Synthesis and optimization of a novel poly‐silicic metal coagulant from coal gangue for tertiary‐treatment of coking wastewater

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