Phenobarbital (PB)-induced changes in blood coagulationrelated parameters in pregnant rats, lactating rats and pups

Abstract

-Effects of repeated administration of phenobarbital (PB) on blood coagulation-related parameters were examined in non-pregnant, pregnant and lactating rats, and also in pups born to PB-treated lactating dams. PB was orally administered at a dose level of 80 mg/kg/day to pregnant (from gestation day (GD) 13), postpartum (from postpartum day (PPD) 7) and non-pregnant rats (from 13 weeks of age) for 7 days. Blood was collected on GD20 or PPD14 to perform blood coagulation examination. Concurrently, the blood coagulation parameters were examined in the pups. Increases in liver weight and/or hepatic cytochrome P450 content were observed in the PB-treated non-pregnant, pregnant and lactating rats. Activated partial thromboplastin time (APTT) was prolonged and anti-thrombin III (ATIII) concentration was increased in the lactating rats, while there were no changes in prothrombin time (PT) or APTT in the non-pregnant and pregnant rats. Moreover, prolongation of PT and APTT and decreases in factors VII and IX activities were observed in their pups. Thus, prolongation of blood coagulation time was con-2 (VK 2 ) on PB-induced changes in blood coagulation-related parameters of both dams and their pups were examined by co-administration with PB and VK 2 to lactating dams. PT and APTT were comparable to the control and PB-induced prolongation of blood coagulation time was improved in the pups while APTT was prolonged in dams, suggesting that VK 2

Effects of pregnancy and lactation on warfarin-induced changes in blood coagulation-related parameters in rats

“…Therefore, the reaction to chemical exposure is possibly different between pregnant or postpartum animals and non-pregnant animals. To date, there are only a few reports of effects of chemical-treatment on blood coagulation-related parameters or blood chemistry parameters in pregnant and lactating animals (Mochizuki et al, 2009a(Mochizuki et al, , 2009b.…”