Phenethyl alcohol induced germination of ascospores of Neurospora

Lingappa, B. T.; Lingappa, Yamuna; Turian, G.

doi:10.1007/bf00409515

Cited by 11 publications

(10 citation statements)

References 17 publications

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“…In fact, the first IMP that was studied in detail, the glycoprotein G of vesicular stomatitis virus, complied well with this set of rules: an NH2-terminal cleaved signal sequence initiates translocation; the translocation is interrupted by a stop-transfer sequence, yielding a bitopic IMP with an NH2-terminal trans domain, a single TMS, and a COOHterminal cis domain (26)(27)(28).…”

Section: Resultsmentioning

confidence: 86%

“…Such a topology requires that certain segments of the polypeptide chain (referred to as trans domains) be translocated from the biosynthetic compartment to the trans side of the membrane and that alternating segments (referred to as cis domains) remain untranslocated on the cis side of the membrane facing the biosynthetic compartment. Translocation, whether of an entire polypeptide chain or of only one segment, is dependent on a signal sequence (2)(3)(4).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Multiple topogenic sequences in bovine opsin.

Audigier¹,

Friedlander²,

Blobel³

1987

Proc. Natl. Acad. Sci. U.S.A.

112

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Bovine opsin, a polytopic integral membrane protein, contains seven transmembrane segments connecting eight hydrophilic domains alternating on each side of the membrane. To localize topogenic sequences that might specify the distinct topology of opsin in the membrane, we constructed various opsin mutants, each containing only one transmembrane segment. Messenger RNAs transcribed from these mutants were translated in a cell-free system supplemented with microsomal membranes. Among six of the seven transmembrane segments of opsin that were analyzed, five were able to function as signal sequences and also expressed stop-transfer sequences of variable strength. By the criteria of extractability at pH 11 and protease sensitivity, the presence of a signal sequence in combination with a "strong" stop-transfer sequence yielded integration into the lipid bilayer of the majority of chains. However, in combination with a "weak" stoptransfer sequence, we observed integration into the lipid bilayer of only some chains, with the others either completely translocated across the membrane or retained in a wateraccessible space in the membrane.Polytopic integral membrane proteins (IMPs) span the lipid bilayer several times and have several hydrophilic domains alternately exposed on both sides of the membrane (1). Such a topology requires that certain segments of the polypeptide chain (referred to as trans domains) be translocated from the biosynthetic compartment to the trans side of the membrane and that alternating segments (referred to as cis domains) remain untranslocated on the cis side of the membrane facing the biosynthetic compartment. Translocation, whether of an entire polypeptide chain or of only one segment, is dependent on a signal sequence (2-4).Translocation of only a segment of the polypeptide chain requires that the translocation process that is initiated by a signal sequence be terminated by a "stop-transfer" sequence (1, 5). Thus, one conceivable mechanism by which polytopic integration could be achieved is by a series of alternating signal and stop-transfer sequences (1, 6).Bovine opsin is a representative of a polytopic IMP. It contains four trans domains, four cis domains, and seven transmembrane segments (TMS) (7,8). Conceivably, opsin could contain four signal sequences (one for each trans domain) and four stop-transfer sequences (one for each cis domain) (1,6). By deletion of selected segments from a bovine opsin cDNA clone and subsequent translation of the mRNAs in a cell-free translation system supplemented with canine microsomal membranes, we have localized two signal sequences in TMS 1 and 6 (6). We report here the analysis of six of the seven TMS of opsin and the finding that five of these were able to function as signal sequences. Moreover, we show that those TMS that contained signal sequences also express strong or weak stop-transfer sequences. MATERIALS AND METHODSConstruction of the Opsin Mutants. Preparation of the inserts. The plasmid pSF1 containing the complete cDNA of opsin (8) inse...

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Section: Resultsmentioning

confidence: 86%

mentioning

confidence: 99%

Multiple topogenic sequences in bovine opsin.

Audigier¹,

Friedlander²,

Blobel³

1987

Proc. Natl. Acad. Sci. U.S.A.

112

View full text Add to dashboard Cite

show abstract

“…Cotranslational processing of the in vitro translocation products was achieved by adding 8 A260 units dog pancreatic microsomal membranes to 1 ml of the translation assay [37 -391. Immunoprecipitation of in vitro translation products was according to Lingappa et al [39].…”

Section: Isolation Of Cdna-clone-selected M R N a : Translation And Imentioning

confidence: 99%

Characterization and Cloning of the mRNAs Specific for the Lactating Mouse Mammary Gland

Hennighausen

Sippel

1982

European Journal of Biochemistry

129

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We have characterized and cloned the lactation-specific mRNAs of mouse mammary glands. The group of eight milk-protein-specific mRNAs were identified (a) by size and antigenic properties of their translation products in vitro and (b) by characterization of their respective cDNA clones. Two x-caseins (43 kDa and 39 kDa) are encoded by mRNAs of 1600 nucleotides and two a-caseins (26 kDa) are encoded by mRNAs of 1450 nucleotides in length. Three smaller caseins, y-casein (23.7 kDa), &casein (21 kDa) and &-casein (14.5 kDa) are synthesized by mRNAs of 880, 1150 and 860 nucleotides. Beside these casein mRNAs a mammary specific 620 nucleotide mRNA codes for a novel acidic whey protein (13.7 kDa).cDNA clones corresponding to the mRNAs for the lactation-specific proteins have been isolated from a mammary-specific cDNA library. Cloned a-casein cDNA hybridizes to both cc-casein specific mRNAs and cloned a-casein cDNAs hybridize with both a-casein specific mRNAs. By RNA blot analysis we show that the cloned cDNAs for mouse a-casein, [)-casein, y-casein and &-casein and the acidic whey protein cross-hybridize with mRNAs of the rat, demonstrating partial sequence homology between the corresponding mRNAs of those species.

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“…Phenylethyl alcohol is a common gas released by bacteria and fungi and exerts antimicrobial activities against various plant pathogenic fungi at very high levels or in cooperation with other volatile compounds [33,40,41,[46][47][48]. Phenylethyl alcohol has rose-like flavors and has been demonstrated to induce germination of ascospores, but suppress conidial germination in Neurospora crassa [49,50]. Phenylethyl alcohol affects membrane permeability, causes the alternation of amino acid and sugar transport systems, and inhibits the synthesis of macromoleculars and thus, is inhibitory to microorganisms [51,52].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Penicillium digitatum and Citrus Green Mold by Volatile Compounds Produced by Enterobacter cloacae

Chen¹,

Peng²

2016

J Plant Pathol Microbiol

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Penicillium digitatum causes green mold decay on citrus fruit, resulting in severe economic losses to citrus growers and packers worldwide. The present study is to evaluate the control of citrus green mold by volatiles produced by Enterobacter cloacae. An E. cloacae strain isolated from plant rhizospheres was able to produce three volatile organic compounds, which were identified as butyl acetate, phenylethyl alcohol, and 4,5-dimethyl-1-hexene by GC/MS chromatography. The volatile compounds produced by E. cloacae inhibited conidial germination and hyphal elongation of P. digitatum and reduced green mold severity. E. cloacae cultured at temperatures ranging from 16°C to 28°C, at pH values ≤6, or in a substrate carrier (sphagnum moss, vermiculite, or perlite) provided superior control against P. digitatum. A laboratory formulation using E. cloacae and perlite protected citrus fruit from green mold up to 22 days and its effectiveness outperformed fungicide application at room temperature (~25°C). The results implicate practical application of E. cloacae as a biofumigant for controlling citrus postharvest decay caused by P. digitatum. Significantly, the study provides a model for future research on how to formulate an effective biocontrol agent for disease management.

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Phenethyl alcohol induced germination of ascospores of Neurospora

Cited by 11 publications

References 17 publications

Multiple topogenic sequences in bovine opsin.

Multiple topogenic sequences in bovine opsin.

Characterization and Cloning of the mRNAs Specific for the Lactating Mouse Mammary Gland

Inhibition of Penicillium digitatum and Citrus Green Mold by Volatile Compounds Produced by Enterobacter cloacae

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