Phenanthrene metabolism by Pseudomonas and Burkholderia strains

Balashova, N. V.; Кошелева, И. А.; Golovchenko, N P; Boronin, A. M.

doi:10.1016/s0032-9592(99)00069-2

Cited by 78 publications

(43 citation statements)

References 11 publications

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“…Pollutant degradation and transformation to more volatile or less toxic forms were commonly used strategies for bacteria to reduce the toxicity of their immediate surroundings (Ford, 1993). In our study, most of denitrifiers were closely related to the orders of Rhizobiales, Rhodobacterales, Burkholderiales and Pseudomonadales through phylogenetic analyses, and it has been reported that some species in these orders (Keum et al, 2006;Johnson et al, 2004;Zhang et al, 2004;Guo et al, 2008;Balashova et al, 1999), and a great number of other bacterial and fungal species in soil (Haritash and Kaushik, 2009) could degrade PAHs through cometabolism. Degradation as well as aging of PAHs could alleviate stress and promote the recovery of denitrification enzyme activity (Maliszewska-Kordybach, 2005).…”

Section: Potential Denitrification Activity and Dynamic Of N 2 Omentioning

confidence: 56%

“…NCW, Thalassopira xianhensis sp. nov. and Pseudomonas putida belonging to the above four groups were isolated from contaminated soils, wastewater, sludge and marine, and these species were able to degrade PAHs and other hydrocarbons (Bodour et al, 2003;Guo et al, 2008;Zhao et al, 2010;Balashova et al, 1999).…”

Section: Shifts In Diversity and Composition Of The Denitrifying Commmentioning

confidence: 99%

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Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

Guo

Deng

Qiao

et al. 2011

Environmental Pollution

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a b s t r a c tToxicity of pyrene on the denitrifiers was studied by spiking an agricultural soil with pyrene to a series of concentrations (0e500 mg kg À1 ) followed by doseeresponse and dynamic incubation experiments. Results showed a positive correlation between potential denitrification activity and copy numbers of denitrifying functional genes (nirK, nirS and nosZ), and were both negatively correlated with pyrene concentrations. Based on the comparison of EC 50 values, denitrifiers harboring nirK, nirS or nosZ gene were more sensitive than denitrification activity, and denitrifiers harboring nirS gene were more sensitive than that harboring nirK or nosZ genes. Seven days after spiking with EC 50 concentration of pyrene, denitrifiers diversity decreased and community composition changed in comparison with the control. Phylogenetic analyses of three genes showed that the addition of pyrene increased the proportion of Bradyrhizobiaceae, Rhodospirillales, Burkholderiales and Pseudomonadales. Some species belonging to these groups were reported to be able to degrade PAHs.

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Section: Potential Denitrification Activity and Dynamic Of N 2 Omentioning

confidence: 56%

Section: Shifts In Diversity and Composition Of The Denitrifying Commmentioning

confidence: 99%

Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

Guo

Deng

Qiao

et al. 2011

Environmental Pollution

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“…But no attempt had been made to use 2-hydroxy-1-naphthoic acid as growth substrate or in a resting cell assay to confirm its involvement in the phenanthrene degradation pathway. Similarly, Balashova et al (1999) reported the accumulation of 1-hydroxy-2-naphthoic acid and 2-hydroxy-1-naphthoic acid as the major metabolites in phenanthrene metabolism in Pseudomonas and Burkholderia spp., but the authors were unable to examine the pathway involved in the metabolism of 2-hydroxy-1-naphthoic acid. Recently, 1-hydroxy-2-naphthoic acid and 2-hydroxy-1-naphthoic acid were reported to be identified as metabolic intermediates in the degradation of phenanthrene by Burkholderia sp.…”

Section: Discussionmentioning

confidence: 88%

“…strain P2, Mycobacterium vanbaalenii PYR-1 and Sinorhizobium sp. C4 has been reported recently (Balashova et al, 1999;Pinyakong et al, 2000;Keum et al, 2006;Kim et al, 2005). These studies revealed that the 1,2-dihydroxyphenanthrene produced in the degradation of phenanthrene was further metabolized to 2-hydroxy-1-naphthoic acid, which was either accumulated as a dead-end metabolite in the culture filtrate or subsequently metabolized by less-characterized minor pathway(s).…”

Section: Introductionmentioning

confidence: 82%

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A novel degradation pathway in the assimilation of phenanthrene by Staphylococcus sp. strain PN/Y via meta-cleavage of 2-hydroxy-1-naphthoic acid: formation of trans-2,3-dioxo-5-(2′-hydroxyphenyl)-pent-4-enoic acid

2007

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Staphylococcus sp. strain PN/Y, capable of utilizing phenanthrene as a sole source of carbon and energy, was isolated from petroleum-contaminated soil. In the degradation of phenanthrene by strain PN/Y, various metabolites, isolated and identified by a combination of chromatographic and spectrometric analyses, revealed a novel phenanthrene assimilation pathway involving 2-hydroxy-1-naphthoic acid. Metabolism of phenanthrene was initiated by the dioxygenation on the 1,2-position of phenanthrene followed by meta-cleavage of phenanthrene-1,2-diol, leading to 2-hydroxy-1-naphthoic acid, which was then processed via a novel meta-cleavage pathway, leading to the formation of trans-2,3-dioxo-5-(29-hydroxyphenyl)-pent-4-enoic acid and subsequently to salicylic acid. In the lower pathway, salicylic acid was transformed to catechol, which was then metabolized by catechol-2,3-dioxygenase to 2-hydroxymuconaldehyde acid, ultimately forming TCA cycle intermediates. The catabolic genes involved in phenanthrene degradation were found to be plasmid-encoded. This detailed study of polycyclic aromatic hydrocarbon (PAH) metabolism by a Gram-positive species involving a unique ring-cleavage dioxygenase in a novel phenanthrene degradation pathway provides a new insight into the microbial degradation of PAHs. INTRODUCTIONPolycyclic aromatic hydrocarbons (PAHs) constitute a group of priority environmental pollutants, which are ubiquitous contaminants in soils and sediments and are of environmental concern because of their toxic, mutagenic and/or carcinogenic effects (Mastrangelo et al., 1996;Marston et al., 2001;Xue & Warshawsky, 2005). In recent years, the biodegradation of PAHs has received considerable attention and a variety of micro-organisms have been reported to play important roles in the process (Pothuluri & Cerniglia, 1994;Shuttleworth & Cerniglia, 1995;Kanaly & Harayama, 2000;Habe & Omori, 2003;Tortella et al., 2005). Bioremediation technologies have increasingly been proposed to decontaminate PAH-contaminated sites (Harayama, 1997;Samanta et al., 2002;Parrish et al., 2004;Vinas et al., 2005).Phenanthrene, a PAH with three condensed rings fused in angular fashion, has a 'bay-region' and a 'K-region' and is often used as a model substrate for studies on the metabolism of carcinogenic PAHs. Over the last 60 years, a number of studies on phenanthrene degradation by several Gram-negative and Gram-positive bacterial species have been reported (Evans et al., 1965;Kiyohara et al., 1976Kiyohara et al., , 1982Kiyohara & Nagao, 1978;Barnsley, 1983;Gibson & Subramanian, 1984;Houghton & Shanley, 1994;Adachi et al., 1999;Samanta et al., 1999), where various pathways and metabolic diversity involved in phenanthrene degradation were documented. In general, the metabolic pathway is initiated by the double hydroxylation of the bay-region of phenanthrene by a dioxygenase enzyme to form cis-3,4-phenanthrenedihydrodiol. The resultant dihydrodiol is then converted by the action of dihydrodiol dehydrogenase to 3,4-dihydroxyphenanthrene, which und...

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Application of Microorganisms in Bioremediation

Thakkar¹,

Vincent²

2021

Bioprospecting of Microorganism‐Based Industrial Molecules

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Phenanthrene metabolism by Pseudomonas and Burkholderia strains

Cited by 78 publications

References 11 publications

Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

A novel degradation pathway in the assimilation of phenanthrene by Staphylococcus sp. strain PN/Y via meta-cleavage of 2-hydroxy-1-naphthoic acid: formation of trans-2,3-dioxo-5-(2′-hydroxyphenyl)-pent-4-enoic acid

Application of Microorganisms in Bioremediation

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