PHEMA-PLLA semi-interpenetrating polymer networks: A study of their swelling kinetics, mechanical properties and cellular behavior

Passos, Marcele Fonseca; Fernández-Gutiérrez, Mar; Vázquez, Blanca; Román, Julio San; Filho, Rubens Maciel

doi:10.1016/j.eurpolymj.2016.10.023

Cited by 17 publications

(8 citation statements)

References 51 publications

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“…The cell viability data are highly positive, indicating that no cell death occurred. According to the ISO 10993-5:2009 standard, there was no compromise on biocompatibility based on these cytocompatibility results [48]. Because of acceptable sample roughness and surface features, cell concentration increased with increasing nHA concentration up to 10 wt% (Figure 7a,b).…”

Section: Degradation Analysismentioning

confidence: 96%

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Comparative Study of Crystallization, Mechanical Properties, and In Vitro Cytotoxicity of Nanocomposites at Low Filler Loadings of Hydroxyapatite for Bone-Tissue Engineering Based on Poly(l-lactic acid)/Cyclo Olefin Copolymer

Nazir

Iqbal

2021

Polymers

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A poly(l-lactic acid)/nanohydroxyapatite (PLLA/nHA) scaffold works as a bioactive, osteoconductive scaffold for bone-tissue engineering, but its low degradation rate limits embedded HA in PLLA to efficiently interact with body fluids. In this work, nano-hydroxyapatite (nHA) was added in lower filler loadings (1, 5, 10, and 20 wt%) in a poly(l-lactic acid)/cyclo olefin copolymer10 wt% (PLLA/COC10) blend to obtain novel poly(l-lactic acid)/cyclo olefin copolymer/nanohydroxyapatite (PLLA/COC10-nHA) scaffolds for bone-tissue regeneration and repair. Furthermore, the structure-activity relationship of PLLA/COC10-nHA (ternary system) nanocomposites in comparison with PLLA/nHA (binary system) nanocomposites was systematically studied. Nanocomposites were evaluated for structural (morphology, crystallization), thermomechanical properties, antibacterial potential, and cytocompatibility for bone-tissue engineering applications. Scanning electron microscope images revealed that PLLA/COC10-nHA had uniform morphology and dispersion of nanoparticles up to 10% of HA, and the overall nHA dispersion in matrix was better in PLLA/COC10-nHA as compared to PLLA/nHA. Fourier transformation infrared spectroscopy (FTIR), powder X-ray diffraction (XRD), and differential scanning calorimetry (DSC) studies confirmed miscibility and transformation of the α-crystal form of PLLA to the ά-crystal form by the addition of nHA in all nanocomposites. The degree of crystallinity (%) in the case of PLLA/COC10-nHA 10 wt% was 114% higher than pure PLLA/COC10 and 128% higher than pristine PLLA, indicating COC and nHA are acting as nucleating agents in the PLLA/COC10-nHA nanocomposites, causing an increase in the degree of crystallinity (%). Moreover, PLLA/COC10-nHA exhibited 140 to 240% (1–20 wt% HA) enhanced mechanical properties in terms of ductility as compared to PLLA/nHA. Antibacterial activity results showed that 10 wt% HA in PLLA/COC10-nHA showed substantial activity against P. aeruginosa, S. aureus, and L. monocytogenes. In vitro cytocompatibility of PLLA/COC10 and PLLA nanocomposites with nHA osteoprogenitor cells (MC3T3-E1) and bone mesenchymal stem cells (BMSC) was evaluated. Both cell lines showed two- to three-fold enhancement in cell viability and 10- to 30-fold in proliferation upon culture on PLLA/COC10-nHA as compared to PLLA/nHA composites. It was observed that the ternary system PLLA/COC10-nHA had good dispersion and interfacial interaction resulting in improved thermomechanical and enhanced osteoconductive properties as compared to PLLA/nHA.

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Section: Degradation Analysismentioning

confidence: 96%

“…The enhancement of swelling was attributed to the amorphous nature of COC copolymer for accommodating water molecules due to the disordered arrangement of COC polymeric network. Therefore, the additional nHA could fine tune swelling properties of the nanocomposites for bone-tissue engineering [48].…”

Section: Swelling Analysis Of Nanocompositesmentioning

confidence: 99%

Comparative Study of Crystallization, Mechanical Properties, and In Vitro Cytotoxicity of Nanocomposites at Low Filler Loadings of Hydroxyapatite for Bone-Tissue Engineering Based on Poly(l-lactic acid)/Cyclo Olefin Copolymer

Nazir

Iqbal

2021

Polymers

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“…To eliminate the thermal history of the material, a heating ramp from 25°C to 250°C at a rate of 20°C/min was performed, followed by a cooling to 25°C. Afterward, the samples were reheated to 250°C at 20°C/min, under a nitrogen atmosphere as a purge gas with a flow rate of 50 mL/min [16]. Calibration was made with indium standards.…”

Section: Characterizationmentioning

confidence: 99%

“…In samples A and B, the F g increased with solvent volume fraction decreasing in an aqueous mixture. In general, around 1 wt% diester ethylene glycol dimethacrylate (EGDMA) is present as a byproduct in the commercial monomer [16], and it works as a cross-linker [35]. us, it is seen that HEMA increases and cross-linker content increased F g .…”

Section: Infrared Radiation-induced Synthesis and Characterization Ofmentioning

confidence: 99%

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PHEMA Hydrogels Obtained by Infrared Radiation for Cartilage Tissue Engineering

Passos

Carvalho

Rodrigues³

et al. 2019

International Journal of Chemical Engineering

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Although the exposure of polymeric materials to radiation is a well-established process, little is known about the relationship between structure and property and the biological behavior of biomaterials obtained by thermal phenomena at 1070 nm wavelength. This study includes results concerning the use of a novel infrared radiation source (ytterbium laser fiber) for the synthesis of poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogel in order to produce medical devices. The materials were obtained by means of free radical polymerization mechanism and evaluated regarding its cross-linking degree, polymer chain mobility, thermal, and mechanical properties. Their potential use as a biomaterial toward cartilage tissue was investigated through incubation with chondrocytes cells culture by dimethylmethylene blue (DMMB) dye and DNA quantification. Differential scanning calorimetry (DSC) results showed that glass transition temperature (Tg) was in the range 103°C–119°C, the maximum degree of swelling was 70.8%, and indentation fluency test presented a strain of 56%–85%. A significant increase of glycosaminoglycans (GAGs) concentration and DNA content in cells cultured with 40 wt% 2-hydroxyethyl methacrylate was observed. Our results showed the suitability of infrared laser fiber in the free radicals formation and in the rapid polymer chain growth, and further cross-linking. The porous material obtained showed improvements concerning cartilage tissue regeneration.

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Hydroxyapatite and Whitlockite Incorporated Cellulose Reinforced Poly-Caprolactone (PCL): Biomimetic Nanocomposites for Bone Tissue Engineering Applications

et al. 2022

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PHEMA-PLLA semi-interpenetrating polymer networks: A study of their swelling kinetics, mechanical properties and cellular behavior

Cited by 17 publications

References 51 publications

Comparative Study of Crystallization, Mechanical Properties, and In Vitro Cytotoxicity of Nanocomposites at Low Filler Loadings of Hydroxyapatite for Bone-Tissue Engineering Based on Poly(l-lactic acid)/Cyclo Olefin Copolymer

Comparative Study of Crystallization, Mechanical Properties, and In Vitro Cytotoxicity of Nanocomposites at Low Filler Loadings of Hydroxyapatite for Bone-Tissue Engineering Based on Poly(l-lactic acid)/Cyclo Olefin Copolymer

PHEMA Hydrogels Obtained by Infrared Radiation for Cartilage Tissue Engineering

Hydroxyapatite and Whitlockite Incorporated Cellulose Reinforced Poly-Caprolactone (PCL): Biomimetic Nanocomposites for Bone Tissue Engineering Applications

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