PHB and Other Polhydroxyalkanoic Acids

Production of polyhydroxyalkanoates (PHAs) by Bacillus subtilis and Escherichia coli grown on cane molasses fortified with ethanol

“…After fermentation, bacterial cells with PHAs are normally separated from the medium by centrifugation (Verlinden et al 2007). Following this, extraction and purification can be done by several methods (Steinbuchel 1996). Most of the existing methods for PHA recovery involve the use of organic solvents, such as acetone and chloroform.…”

Cyanobacterial polyhydroxyalkanoates: an alternative source for plastics

“…Using fermentation technology, the maximum PHB that can be accumulated by heterotrophic bacteria amounts to up to 80% of the dry cell weight (dcw; Reddy et al, 2003). To have a clear picture of bacterial PHA production, readers are referred to Lafferty, Korsatko, and Korsatko (1988); Anderson and Dawes (1990); ; Fuller and Lenz (1990); Lee (1996); Sasikala (1996); Steinbüchel (1996) ;Braunegg, Gilles, and Klaus, (1998); Lee, Choi, and Wong (1999); Sudesh, Abe, and Doi (2000); Ishizaki, Tanaka, and Taga (2001); Serafim et al (2001), Reddy et al (2003), Khanna and Srivastava (2005), Lenz and Marchessault (2005), Valappil et al (2007), Suriyamongkol et al (2007), Chen (2009), Keshavarz and Roy (2010) and Sudesh et al (2011). Despite being under investigation for such a long time, large-scale PHA production is still to be optimized.…”

Inactivation of type I polyhydroxyalkanoate synthase in Aeromonas hydrophila resulted in discovery of another potential PHA synthase

“…Only a few reports are available of bacteria, for example, Rhodospirillum rubrum [2], Rhodocyclus gelatinosus [16], Aeromonas hydrophila [19], Rhodococcus rubber [8] and some Pseudomonad strains [4,12,22,24], which produce PHA consisting of both scl and mcl PHA.…”

“…Depending on the number of carbon atoms in monomer units, PHA can be divided into two groups: short-chain-length (scl) PHA with C 3 to C 5 monomer units and medium-chain-length (mcl) PHA with C 6 to C 14 monomer units [22]. Only a few reports are available of bacteria, for example, Rhodospirillum rubrum [2], Rhodocyclus gelatinosus [16], Aeromonas hydrophila [19], Rhodococcus rubber [8] and some Pseudomonad strains [4,12,22,24], which produce PHA consisting of both scl and mcl PHA.…”