Phasin interactome reveals the interplay of<scp>PhaF</scp>with the polyhydroxyalkanoate transcriptional regulatory protein<scp>PhaD</scp>in<i>Pseudomonas putida</i>

Tarazona, Natalia A.; Hernández‐Arriaga, Ana M.; Kniewel, R.; Prieto, María Auxiliadora

doi:10.1111/1462-2920.15175

Cited by 20 publications

(23 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Very recently, FadD1 (PP_4549) has been identified as a prominent GAP in the granule proteome of P. putida KT2440—suggesting that biochemical reactions involved in the entire PHA cycle (i.e., polymer synthesis, polymer degradation, and monomer activation) are carried out in the proximity of the granule surface, thus facilitating a continuous and dynamic turnover of PHA. [ 57 ]…”

Section: β‐Oxidation Orchestrates Pha Biosynthesis From Structurally mentioning

confidence: 99%

See 1 more Smart Citation

Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates

Mezzina

Manoli

Prieto

et al. 2020

Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

Growing environmental concern sparks renewed interest in the sustainable production of (bio)materials that can replace oil‐derived goods. Polyhydroxyalkanoates (PHAs) are isotactic polymers that play a critical role in the central metabolism of producer bacteria, as they act as dynamic reservoirs of carbon and reducing equivalents. PHAs continue to attract industrial attention as a starting point toward renewable, biodegradable, biocompatible, and versatile thermoplastic and elastomeric materials. Pseudomonas species have been known for long as efficient biopolymer producers, especially for medium‐chain‐length PHAs. The surge of synthetic biology and metabolic engineering approaches in recent years offers the possibility of exploiting the untapped potential of Pseudomonas cell factories for the production of tailored PHAs. In this article, an overview of the metabolic and regulatory circuits that rule PHA accumulation in Pseudomonas putida is provided, and approaches leading to the biosynthesis of novel polymers (e.g., PHAs including nonbiological chemical elements in their structures) are discussed. The potential of novel PHAs to disrupt existing and future market segments is closer to realization than ever before. The review is concluded by pinpointing challenges that currently hinder the wide adoption of bio‐based PHAs, and strategies toward programmable polymer biosynthesis from alternative substrates in engineered P. putida strains are proposed.

show abstract

Section: β‐Oxidation Orchestrates Pha Biosynthesis From Structurally mentioning

confidence: 99%

“…[ 22b ] Very recently, a direct interaction of PhaF and PhaD has been demonstrated—suggesting the existence of a multicomplex regulatory system formed by PHA granules, a PhaD–PhaF complex, and the target DNA. [ 57 ]…”

Section: Regulation Of Pha Synthesis In P Putida Kt2440: From Transcmentioning

confidence: 99%

Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates

Mezzina

Manoli

Prieto

et al. 2020

Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…We will first review the most recent findings on PHA granules biogenesis achieved by studying Ralstonia eutropha H16 (also designated as Hydrogenomonas eutropha H16, Alcaligenes eutrophus H16, Wautersia eutropha H16, and Cupriavidus necator H16). Most statements apply to PHB granules of other species and to PHA granules consisting of mcl-PHA (for earlier overviews on PHB/PHA granules, see the following references (Pötter and Steinbüchel 2006;Kuchta et al 2007;Jendrossek and Pfeiffer 2014;Prieto et al 2016;Tarazona et al 2020).…”

Section: Phb Synthesis and Supramolecular Structure Of Granulesmentioning

confidence: 99%

“…The PHA granules of mcl-PHA accumulating species have a similar structure consisting of a polymer core and several polymer surface-attached proteins. Most studies on the structure and composition of mcl-PHA granules have been made on Pseudomonas putida (Prieto et al 2016;Tarazona et al, 2020).…”

Section: Phb Granule-associated Proteinsmentioning

confidence: 99%

The protective role of PHB and its degradation products against stress situations in bacteria

Müller-Santos

Koskimäki

Alves

et al. 2020

FEMS Microbiology Reviews

View full text Add to dashboard Cite

Many bacteria produce storage biopolymers that are mobilized under conditions of metabolic adaptation, for example, low nutrient availability and cellular stress. Polyhydroxyalkanoates (PHA) are often found as carbon storage in Bacteria or Archaea, and polyhydroxybutyrate (PHB) is the more frequent. Bacteria usually produce PHB upon availability of a carbon source and limitation of another essential nutrient. Therefore, it is widely believed that the function of PHB is to serve as a mobilizable carbon repository when bacteria face carbon limitation, supporting their survival. However, recent findings indicate that bacteria switch from PHB synthesis to mobilization under stress conditions such as thermal and oxidative shock. The mobilization products, 3-hydroxybutyrate and its oligomers, show a protective effect against protein aggregation and cellular damage caused by reactive oxygen species and heat shock. Thus, bacteria should have an environmental monitoring mechanism directly connected to the regulation of the PHB metabolism. Here, we review the current knowledge on PHB physiology together with a summary of recent findings on novel functions of PHB in stress resistance. Potential applications of these new functions are also presented.

show abstract

“…The carboxy terminus containing histone‐like DNA‐binding domain is natively unfolded in the absence of DNA but acquires a super helical structure upon its non‐specific binding to DNA. It has been demonstrated that PhaF segregates the PHA granules among the two daughter cells during cell division process by binding to both PHA granules and nucleoid region (Galán et al ., 2011 ; Tarazona et al ., 2020 ). The central core of PhaF contains a leucine zipper motif, which is involved in the oligomerization of PhaF protein.…”

Section: Regulation Of Pha Synthesismentioning

confidence: 99%

An updated overview on the regulatory circuits of polyhydroxyalkanoates synthesis

Mitra

Chen

et al. 2021

Microbial Biotechnology

View full text Add to dashboard Cite

Polyhydroxyalkanoates (PHA) are a promising and sustainable alternative to the petroleum-based synthetic plastics. Regulation of PHA synthesis is receiving considerable importance as engineering the regulatory factors might help developing strains with improved PHA-producing abilities. PHA synthesis is dedicatedly regulated by a number of regulatory networks. They tightly control the PHA content, granule size and their distribution in cells. Most PHAaccumulating microorganisms have multiple regulatory networks that impart a combined effect on PHA metabolism. Among them, several factors ranging from global to specific regulators, have been identified and characterized till now. This review is an attempt to categorically summarize the diverse regulatory circuits that operate in some important PHA-producing microorganisms. However, in several organisms, the detailed mechanisms involved in the regulation of PHA synthesis is not well-explored and hence further research is needed. The information presented in this review might help researcher to identify the prevailing research gaps in PHA regulation.

show abstract

Phasin interactome reveals the interplay ofPhaFwith the polyhydroxyalkanoate transcriptional regulatory proteinPhaDinPseudomonas putida

Abstract: proteins. Analysis of the interaction of PhaF and PhaD with the phaI promoter by EMSA suggested a new role for PhaF in interacting with PhaD and raises new questions on the regulatory system controlling pha gene expression.

Cited by 20 publications

References 40 publications

Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates

Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates

The protective role of PHB and its degradation products against stress situations in bacteria

An updated overview on the regulatory circuits of polyhydroxyalkanoates synthesis

Contact Info

Product

Resources

About