Phaseolamin treatment prevents oxidative stress and collagen deposition in the hearts of streptozotocin-induced diabetic rats

Abstract

The development of cardiovascular complications in patients with diabetes is often associated with an imbalance between reactive oxygen species and antioxidant systems. This imbalance can contribute to high cardiac collagen content, which increases cross-linking and the stiffness of the myocardium. In this study, the protective effect of phaseolamin against damage under oxidative stress and collagen deposition in the cardiac tissue in association with diabetes was evaluated. Non-diabetic and diabetic animals were distributed into groups and treated for 20 days with commercial phaseolamin. The phaseolamin treatment increased total antioxidant activity but reduced the following in diabetic rats: (a) hyperglycaemic state, (b) catalase and superoxide dismutase activity and (c) tissue damage caused by lipid peroxidation. Additionally, the phaseolamin treatment attenuated the collagen levels compared to non-treated diabetic rats. Thus, the short-term anti-hyperglycaemic effect of the phaseolamin treatment may prevent the initial changes caused by oxidative stress and the deposition of collagen, as well as reduce the incidence of heart complications.

Phaseolus vulgaris L. Extract: Alpha-Amylase Inhibition against Metabolic Syndrome in Mice

“…This enzyme was discovered in 1945 by [9], and was characterized and named “phaseolamin” in 1975 by [10]. Since then, phaseolamin was tested for its efficacy as a starch blocker and purified extracts were used as human dietary supplements with anti-hyperglycaemic and anti-obesity purpose [11]. The results were not as expected, probably because of an insufficient inhibitory activity on human alpha-amylase.…”

Clinical applications of amylase: Novel perspectives

“…Oliveira et al found that phaseolamin can be used to control diabetes due to its anti-hyperglycemic effect. 100 Another study did not find any effect on the glycemic state, although this could be due to the low dose used in the latter study. 101 Interestingly, the product of amylase activity (glucose) has been shown to inhibit effectively the production of the main toxins responsible for the onset and progression of gas gangrene, lending some credence to claims of antibacterial efficacy of sugar based wound dressings such as Manuka Hone.…”

It’s No Has Bean: A Review of the Effects of White Kidney Bean Extract on Body Composition and Metabolic Health

“…Despite the promising findings noted above, not all studies have reported a reduction in body weight/reduced weight gain in animals supplemented with WKBE [ 29 , 30 , 31 ]. Further research is therefore warranted to elucidate specific factors which may account for the disparate findings reported in the literature, which could include the activity, purity, dose and duration of the supplement administered [ 32 ]; the health status and type or breed of animals studied [ 40 ]; and the quality of the research (e.g., the presence/nature of a control group, sample size, blinding of researchers) [ 41 ]. In addition, whilst animal model investigations provide useful insight into the potential effects of WKBE on body composition, it is important to consider how well these findings translate into humans, especially when considering a condition such as obesity, which has a complex and multifaceted aetiology.…”

“…Studies investigating the impact of WKBE on blood biochemistry have primarily focused on blood glucose, triglycerides and cholesterol. In both mice and rats supplemented with a variety of different WKBEs and administered doses between 500 mg/kg and 4 g/kg, improvements in blood glucose have been reported when compared with the control groups [ 31 , 34 , 35 , 36 , 40 , 42 ]. Despite reductions in blood glucose, Tormo and colleagues did not observe accompanying improvements in plasma insulin levels [ 35 , 36 ].…”

“…In a rat model of diabetes, which is frequently associated with cardiovascular complications due to a greater number of reactive oxygen species and an impaired antioxidant system, Oliveira et al [ 40 ] evaluated the effects of WKBE on oxidative stress-induced damage and collagen deposition in cardiac tissue. The treatment of diabetic rats with 100, 500 and 1500 mg/kg WKBE for 20 days increased the total antioxidant status of cardiac tissue and reduced the markers of oxidative stress (superoxidase dismutase and catalase activity) and concentrations of malondialdehyde (only with highest dose) when compared with untreated diabetic rats [ 40 ]. These effects of WKBE were accompanied by reduced collagen deposition, which was increased in the hearts of untreated diabetic rats.…”