Phaseic acid: Occurrence in cotton fruit; Acceleration of abscission

Davis, Larry A.; Lyon, J. L.; Addicott, Fredrick T.

doi:10.1007/bf00386615

Cited by 26 publications

(4 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another report phaseic acid was found to occur naturally in cotton fruit (7). It is interesting to observe that ABA application to the leaves of cotton in our experiments (14,15) that in cotton tissues 99% of the radioactivity is recovered as ABA.…”

Section: Discussionsupporting

confidence: 54%

Absorption and Distribution of High Specific Radioactivity 2-¹⁴C-Abscisic Acid in Cotton Seedlings

Shindy

Asmundson²,

Smith³

et al. 1973

Plant Physiol.

View full text Add to dashboard Cite

High specific radioactivity (26.3 mc/mmole) racemic 2-14C-abscisic acid was synthesized. An aliquot of abscisic acid, 1.2 X 10'-M in aqueous methanolic solution, was applied to the surface of either a cotyledon or the first true leaf of 8-to 32-dayold cotton seedlings (Gossypium hirsutum L.). After various intervals (6-192 hours), the seedlings were processed for autoradiography, counting, and identification of the radioactivity. After 6 hours, radioactivity was observed moving basipetally out of the treated leaf toward the roots. Four days later, radioactivity could be detected throughout the whole seedling. After 8 days, 10% of the recovered radioactivity was found in the roots, and 80% remained in the treated leaf blade. Neither leaf type nor age had any effect on the abscisic acid movement or pattern of distribution. Isolated radioactivity from the roots was identified as abscisic acid, based on comparison with an authentic standard by thin layer chromatography, gas-liquid chromatography, or gas-liquid chromatography-mass spectrometry.Abscisic acid has been isolated from cotton plants and identified (6, 24, 25). It has also been found in a large number of plant species (3, 11,18,22,23 MATERIALS AND METHODS Synthesis of High Specific Radioactivity 2-14C-Abscisic Acid. Although a synthesis of 1-"4C-methyl (triphenylphosphoranylidene) acetate has been published (5), the description given is incomplete, and the yield of the 1-4C-compound reported is lower than the yields in the macrosynthesis (17). A similar Wittig reagent was prepared with good yield as follows. Ethyl-2-"C-bromoacetate (21.0 mg) 28.7 mc/mmole, (ICN-Tracerlab) in 0.25 ml of benzene was transferred to a 15-ml graduated centrifuge tube, and the shipping vial was rinsed five times with 0.45 ml of benzene. Triphenylphosphine (132 mg, 0.503 mmole) was added, dissolved in the benzene, and the tube was covered with aluminum foil and allowed to stand at room temperature for 24 hr. The tube was then spun for 15 min at 3000 rpm, and the benzene was decanted. Fresh benzene (2.5 ml) was added, the tube was shaken and then centrifuged again for 15 min at 3000 rpm, and the benzene rinse was decanted. The tube was then dried 2 hr in a vacuum oven at about 250 mm Hg and 40 C to yield 50.22 mg of salt (93.0% of theory). Previous trial runs had given quantitative yields. The salt was dissolved in 2.5 ml of distilled water, and a drop each of 0.1% phenolphthalein alcohol solution and 1% Tween 20 solution was added followed by 4 drops of 10% KOH to a pink end point. The precipitated ethyl (triphenylphosphoranylidene)-2-'4C-acetate was spun down for 15 min at 3000 rpm, the supernatant was decanted, the precipitate was rinsed with 2.5 ml of water and recentrifuged, and the water was decanted. The product was then dried overnight at 250 mm Hg and 60 C to yield 35.67 mg, 87.5% of theory for this step and 81.4% over-all. Trial runs had given 84 + 2% of theory in this step.Next, 4-(2, 6, 6-trimethyl-1 -hydroxy-4-keto-2-cyclohexen-1-yl)-3-buten-2-one, 0.1024 mmole...

show abstract

Section: Discussionsupporting

confidence: 54%

Absorption and Distribution of High Specific Radioactivity 2-¹⁴C-Abscisic Acid in Cotton Seedlings

Shindy

Asmundson²,

Smith³

et al. 1973

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Plant species in which PA has been identified include: Phaseolus multijlorus (MacMillan and Pryce 1969); Gossypium hirsutum (Davis et al 1972); Phaseolus vulgaris (Tinelli et al 1973); Bryophyllum daigremontianum ) and as a rearrangement product of 'metabolite C' in Lycopersicon esculentum (Milborrow 1969). It has very slight growth-inhibiting properties (11200 th that of ABA) in a wheat embryo germination assay (Milborrow 1969) and negligible activity in a bean embryo growth assay (Walton and Sondheimer 1972), while it has about one-tenth the activity of ABA in abscission acceleration (Davis et al 1972). The only PA available to us has been that extracted from grape leaves and we have yet to determine its effect on stomata1 closure.…”

Section: Discussionmentioning

confidence: 99%

Internal Control of Stomatal Physiology and Photosynthesis. I. Stomatal Regulation and Associated Changes in Endogenous Levels of Abscisic and Phaseic Acids

Loveys¹,

Kriedemann²

1974

Functional Plant Biol.

View full text Add to dashboard Cite

Both environmental factors and manipulative treatments (such as fruit excision or stem cincturing) were found to alter gaseous diffusion resistances in grape vine foliage. These responses have been a.nalysed in terms of the hormonal physiology of Vitis vinifera L. leaf tissue.Environmental factors such as moisture stress or photoperiod alterations which contributed towards an increase in stomatal resistance (rs) were correlated with increased levels of endogenous abscisic acid (ABA) and phaseic acid (PA) in mature foliage. Conversely, treatments which elicited a decrease in rs were associated with lower levels of both ABA and PA. For example, rs had increased from 1.41 to 7.14 s cm-l 7 days after fruit removal and stem cincturing, while ABA and PA levels rose by 50 and 370 % respectively. This increase in endogenous ABA was not a consequence of decreased leaf water potential; moisture status was actually improved after treatment.It is proposed that changes in endogenous levels of ABA, and possibly PA, constitute a mechanism for regulating gas exchange in these perennial plants.

show abstract

“…The accumulation of DPA in bean seeds (24), as well as the continuous formation of PA and DPA in water-stressed leaves (5), indicates that this pathway has an active role in regulating total ABA concentration within the plant. The decreased activity of PA in bioassay systems for growth inhibition (9,22), abscission promotion (1,4), and stomatal closure (7) suggests that the DPA pathway has a role in regulating the hormonal activity of ABA. The recent observation that PA inhibits photosynthesis, while ABA does not, indicates that the DPA pathway may also play a role in the activation of physiological activities (7).…”

mentioning

confidence: 99%

Abscisic Acid Metabolism by a Cell-free Preparation from Echinocystis lobata Liquid Endoserum

Gillard

Walton²

1976

Plant Physiol.

105

View full text Add to dashboard Cite

The in vivo feeding of 2-'4C-ABA to embryonic bean axes (Phaseolus vulgaris L.) has established PA2 and DPA as products in one ABA metabolic pathway (21,23). The accumulation of DPA in bean seeds (24), as well as the continuous formation of PA and DPA in water-stressed leaves (5), indicates that this pathway has an active role in regulating total ABA concentration within the plant. The decreased activity of PA in bioassay systems for growth inhibition (9, 22), abscission promotion (1, 4), and stomatal closure (7) suggests that the DPA pathway has a role in regulating the hormonal activity of ABA. The recent observation that PA inhibits photosynthesis, while ABA does not, indicates that the DPA pathway may also play a role in the activation of physiological activities (7). The actual role which the DPA pathway plays in regulating ABA levels requires the investigation of the enzymes involved. The goal of the present investigation was to establish a cell-free enzyme system capable of metabolizing ABA by the DPA pathway.

show abstract

Phaseic acid: Occurrence in cotton fruit; Acceleration of abscission

Cited by 26 publications

References 10 publications

Absorption and Distribution of High Specific Radioactivity 2-¹⁴C-Abscisic Acid in Cotton Seedlings

Absorption and Distribution of High Specific Radioactivity 2-¹⁴C-Abscisic Acid in Cotton Seedlings

Internal Control of Stomatal Physiology and Photosynthesis. I. Stomatal Regulation and Associated Changes in Endogenous Levels of Abscisic and Phaseic Acids

Abscisic Acid Metabolism by a Cell-free Preparation from Echinocystis lobata Liquid Endoserum

Contact Info

Product

Resources

About

Phaseic acid: Occurrence in cotton fruit; Acceleration of abscission

Cited by 26 publications

References 10 publications

Absorption and Distribution of High Specific Radioactivity 2-14C-Abscisic Acid in Cotton Seedlings

Absorption and Distribution of High Specific Radioactivity 2-14C-Abscisic Acid in Cotton Seedlings

Internal Control of Stomatal Physiology and Photosynthesis. I. Stomatal Regulation and Associated Changes in Endogenous Levels of Abscisic and Phaseic Acids

Abscisic Acid Metabolism by a Cell-free Preparation from Echinocystis lobata Liquid Endoserum

Contact Info

Product

Resources

About

Absorption and Distribution of High Specific Radioactivity 2-¹⁴C-Abscisic Acid in Cotton Seedlings

Absorption and Distribution of High Specific Radioactivity 2-¹⁴C-Abscisic Acid in Cotton Seedlings