Phase-transition thresholds and vaporization phenomena for ultrasound phase-change nanoemulsions assessed via high-speed optical microscopy

Sheeran, Paul S.; Matsunaga, Terry O.; Dayton, Paul A.

doi:10.1088/0031-9155/58/13/4513

Cited by 88 publications

(112 citation statements)

References 61 publications

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“…This circulation of the dynamic system has been reported by Sheeran et al [45] and observed with PFP and PFH nanoparticle droplets in water at 25 °C and 37 °C [46].…”

Section: Bubble Growthsupporting

confidence: 82%

“…The phase shift capability of PFC nanoparticles will decrease with increasing ultrasound frequency and surface tension. Many of these results have been confirmed with experimental observations [34,45,53].…”

Section: Acoustic Droplet Vaporizationsupporting

confidence: 68%

“…For small droplets trapped by elastic lipid shells, however, even though the vapor pressure of liquid droplets is greater than its surrounding pressure in the surrounding liquid, the small droplets still have not changed to gas because of Laplace pressure, which contributes to the local pressure imposed on the interior fluid of droplets due to the surface tension [44][45][46].…”

Section: Thermodynamics Of Vaporizationmentioning

confidence: 99%

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Phase-transition Perfluorocarbon Nanoparticles for Ultrasound Molecular Imaging and Therapy

Xu¹,

Cao²,

Xu³

et al. 2015

Nano BioMed ENG

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Recently, the advances in perfluorocarbon nanoparticles have been introduced to expand the diagnostic and therapeutic capability of ultrasound molecular imaging, a non-invasive diagnostic imaging, which passed from robust anatomical presentation to detection of physiological processes and recognition of specific tissue epitopes at the cellular level. The good properties of perfluorocarbon nanoparticles, such as nontoxicity, small size, phase-shift capability, etc., have been resulted in tremendous potential prospects in clinical application. Herein, this review focuses on the mechanisms of perfluorocarbon nanoparticles in terms of phase transition and ultrasonic theranostic. And the potential applications of perfluorocarbon nanoparticles in ultrasound medicine are also discussed.

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“…This circulation of the dynamic system has been reported by Sheeran et al [45] and observed with PFP and PFH nanoparticle droplets in water at 25 °C and 37 °C [46].…”

Section: Bubble Growthsupporting

confidence: 82%

Section: Acoustic Droplet Vaporizationsupporting

confidence: 68%

Section: Thermodynamics Of Vaporizationmentioning

confidence: 99%

See 1 more Smart Citation

Phase-transition Perfluorocarbon Nanoparticles for Ultrasound Molecular Imaging and Therapy

Xu¹,

Cao²,

Xu³

et al. 2015

Nano BioMed ENG

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“…To overcome the size/circulation limitations of microbubbles, researchers have begun using phase change contrast agents (PCCAs), which are injected intravenously as liquid nanodroplets, migrate to the target site, and vaporize into gas microbubbles when exposed to a threshold negative pressure using ultrasound [7]. While in the liquid state, PCCAs can persist in circulation.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound imaging from vaporization signals emitted by phase change contrast agents

Arena

Novell

Sheeran

et al. 2014

2014 IEEE International Ultrasonics Symposium

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Phase change contrast agents (PCCAs) exhibit a unique acoustic signature during the transition from a liquid droplet to a gas microbubble. Here, we demonstrate that this event can be used to generate an ultrasound image, and that the signal can be separated from that of a conventional microbubble. This presents a new opportunity to monitor PCCA activation in both diagnostic and therapeutic applications. A confocal, dualfrequency transducer was used to transmit 2 cycle, Gaussian enveloped sinusoids at 8 MHz and passively receive at 1 MHz. PCCAs were continuously infused through a microcellulose tube (250 μm diameter). At low pressures, vaporization signals from PCCAs were of significantly higher energy than signals emitted from the equivalent microbubble formulation. Specifically, when a peak negative pressure (PNP) of 0.51 MPa was transmitted, the contrast-to-noise ratio (CNR) was 18.94 dB for PCCAs and 2.28 dB for control microbubbles. As the PNP was increased to 0.76 MPa, these values changed to 22.1 dB and 9.73 dB, respectively. Time-domain averaging (TDA) helped to increase the separation of PCCA and microbubble signals. After TDA, the CNR at 0.76 MPa was 23.79 dB for PCCAs and 1.72 dB for microbubbles. The lateral resolution of the system was pressure dependent. With increasing pressure, the apparent diameter of the tube increased from 0.74 mm at 0.51 MPa to 1.14 mm at 0.76 MPa. This is due to the fact that the focal zone capable of activating PCCAs expands with increasing pressure.

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“…While a number of studies have focused on the conditions necessary for droplet nucleation 322,328,332,344,370,373 and the vaporization process itself 339,365,374 , only limited information is available regarding the acoustic properties of these newly created microbubbles.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound contrast agents: bubbles drops and particles

Lajoinie¹

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The research described in this thesis is part of the research program NanoNextNL, a micro and nanotechnology consortium of the Government of the Netherlands and 130 partners. This thesis was carried out at the Physics of Fluids group of the Faculty of Science and Technology of the University of Twente. Cover design:As a representation of a crucial goal of this work, the cover depicts a confocal microscopy image showing cells selectively porated by loaded microbubbles upon ultrasound exposure. The porated cells take up a blue fluorescent probe. By Ine Lentacker, Ine De Cock and Guillaume Lajoinie. Guide through the thesisIn this thesis, we investigate different types of innovative agents, designed for biomedical use in a context of molecular imaging. In Chapter 1, we review the agents that arouse the interest of the scientific community, most of them at the research stage of development, together with the experimental methods that can be used to study their interaction with cells. Such in vitro investigations are necessary to test on short scales the impact of the various agents on biological structures.We separate the agents in three main categories. The first group consists of stabilized microbubbles, usually by means of phospholipids that fill up the interfacial area between the gas core and the surrounding liquid (water). Such bubbles are clinically used for 40 years as contrast agents for ultrasound owing to their unique resonance behavior. When irradiated with an ultrasound pulse, a bubble experiences volumetric oscillations, that reemit an ultrasound wave back to the transducer. This process makes them unchallenged ultrasound scatterers. The role of these stable microbubbles in new biomedical research directions is discussed in Chapters 2 to 9.Phospholipid coatings were developed in the last decade to do much more than just prevent bubble dissolution. They are now a crucial tool to enrich the microbubbles with specific markers or drug payloads. In Chapter 2, we investigate the behavior of this coating when the microbubbles are subjected to an ultrasound field. We show that the coating is shed away from the microbubble, which is of major interest for molecular imaging combined with drug delivery using microbubbles. We pursue the idea in Chapter 3 where we investigate how a bubble payload, here liposomes labeled with a fluorescent dye, is released under ultrasound exposure. In Chapter 4, we link these direct observations of shedding of the previous Chapters to the subsequent dissolution of the microbubbles, no longer protected by a phospholipid shell. This dissolution, visible in the ultrasound scatter spectrum can be used to remotely quantify the effect of the microbubbles. In support of these observations, we dedicate the next Chapter (Chapter 5) to physically understand the mechanisms underlying the behavior of the phospholipid coating. The application of these findings in practice requires the production a sufficiently high production rate and with great control of such microbubbles. In Chapter 6, we th...

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Phase-transition thresholds and vaporization phenomena for ultrasound phase-change nanoemulsions assessed via high-speed optical microscopy

Cited by 88 publications

References 61 publications

Phase-transition Perfluorocarbon Nanoparticles for Ultrasound Molecular Imaging and Therapy

Phase-transition Perfluorocarbon Nanoparticles for Ultrasound Molecular Imaging and Therapy

Ultrasound imaging from vaporization signals emitted by phase change contrast agents

Ultrasound contrast agents: bubbles drops and particles

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