Phase transition of DNA-linked gold nanoparticles

Kiang, Ching‐Hwa

doi:10.1016/s0378-4371(02)01775-2

Cited by 52 publications

(78 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DNA-linked nanoparticle assemblies are a novel system in which gold nanoparticles are chemically affixed to known DNA sequences to create DNA "probes" with the capability to self-assemble into aggregates [1][2][3][4][5]. The interaction potential between these colloids are tunable and controllable, which makes these multicomponent complex fluids particularly suitable for studying the link between the interaction potential and phase behavior [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Recent theory has sought to explain these observations [6,23,24]. More study is necessary to fully understand the underlying mechanisms affecting the phase behavior of this system.Here we report experimental observations of the effects of disorder on the melting temperature of the DNA-linked nanoparticle assemblies [2,3]. The basic building block is illustrated in Fig.…”

mentioning

confidence: 99%

“…Linker DNA resulting in unequal DNA duplex lengths introduces disorder and lowers the melting temperature of the nanoparticle system. Comparison with free DNA thermodynamics shows that such an anomalous zigzag pattern does not exist for free DNA duplex melting, which suggests that the disorder introduced by unequal DNA duplex lengths results in this unusual collective behavior of DNA-linked nanoparticle assemblies.DNA-linked nanoparticle assemblies are a novel system in which gold nanoparticles are chemically affixed to known DNA sequences to create DNA "probes" with the capability to self-assemble into aggregates [1][2][3][4][5]. The interaction potential between these colloids are tunable and controllable, which makes these multicomponent complex fluids particularly suitable for studying the link between the interaction potential and phase behavior [6,7].…”

mentioning

confidence: 99%

“…The assembly melting temperature is dependent upon parameters such as particle size, DNA composition, and electrolyte concentrations [1,2]. Recent theory has sought to explain these observations [6,23,24].…”

mentioning

confidence: 99%

“…The melting temperature accesses the stability of systems. DNA gold nanoparticle probes were synthesized using previously described methods [2]. DNA-gold aggregates were formed by mixing probe DNA particles with linker DNA [2][3][4].…”

mentioning

confidence: 99%

See 4 more Smart Citations

Disorder in DNA-Linked Gold Nanoparticle Assemblies

Harris

Kiang

2005

Phys. Rev. Lett.

Self Cite

View full text Add to dashboard Cite

We report experimental observations on the effect of disorder on the phase behavior of DNA-linked nanoparticle assemblies. Variation in DNA linker lengths results in different melting temperatures of the DNA-linked nanoparticle assemblies. We observed an unusual trend of a nonmonotonic "zigzag" pattern in the melting temperature as a function of DNA linker length. Linker DNA resulting in unequal DNA duplex lengths introduces disorder and lowers the melting temperature of the nanoparticle system. Comparison with free DNA thermodynamics shows that such an anomalous zigzag pattern does not exist for free DNA duplex melting, which suggests that the disorder introduced by unequal DNA duplex lengths results in this unusual collective behavior of DNA-linked nanoparticle assemblies.DNA-linked nanoparticle assemblies are a novel system in which gold nanoparticles are chemically affixed to known DNA sequences to create DNA "probes" with the capability to self-assemble into aggregates [1][2][3][4][5]. The interaction potential between these colloids are tunable and controllable, which makes these multicomponent complex fluids particularly suitable for studying the link between the interaction potential and phase behavior [6,7]. Similar colloidal phase transitions have also been used to detect the protein interactions at membrane surfaces [8]. On the other hand, the dynamics of DNA melting and hybridization in DNA replication and transcription is also a subject of intense investigation [9,10].The change in optical property upon aggregation makes DNA-linked nanoparticle systems a potential tool in future DNA detection technology [11,12]. DNA detection is important in medical research for applications such as detection of genetic diseases, RNA profiling, and biodefense [13][14][15][16][17]. The DNA-linked nanoparticle detection systems utilize the sequencedependent hybridization of DNA for accuracy and the optical properties of colloidal gold for sensitivity to create a DNA detection method that changes color upon the introduction of a specific DNA sequence. Study of these DNA-gold nanoparticle assemblies is warranted to gain a fundamental understanding of DNA hybridization in confined geometries, as well as to probe the potential of this and similar systems for practical applications in biotechnology [18,19].Much like other colloidal suspensions [20][21][22], the DNA-gold nanoparticle system has been shown to exhibit interesting phase behavior. The assembly melting temperature is dependent upon parameters such as particle size, DNA composition, and electrolyte concentrations [1,2]. Recent theory has sought to explain these observations [6,23,24]. More study is necessary to fully understand the underlying mechanisms affecting the phase behavior of this system.Here we report experimental observations of the effects of disorder on the melting temperature of the DNA-linked nanoparticle assemblies [2,3]. The basic building block is illustrated in Fig. 1. Disorder in the DNA duplex length was introduced by choosing linker...

show abstract