Phase separation of polymerized mixed liposomes: analysis of release behavior of entrapped molecules with skeletonization

Takeoka, Shinji; Sakai, Hiromi; Ohno, Hiroyuki; Tsuchida, Eishun

doi:10.1021/ma00006a011

Cited by 8 publications

(16 citation statements)

References 13 publications

(14 reference statements)

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“…16 Several methods may be used. The most popular are gel filtration, [17][18][19][20][21] ultrafiltration, [21][22][23] and ultracentrifugation. 24,25 Once the free fraction is separated from the nanoparticles bound fraction, the evaluation of the encapsulation rate is determined by the appropriate analytical method (GPC, 26 spectrophotometry UV/vis, 27 radioactivity measurement, 27 .…”

Section: Tyrosine Entrapment In Nanoparticlesmentioning

confidence: 99%

Nanoparticles from vesicles polymerization. II. Evaluation of their encapsulation capacity

Poulain

Nakache

1998

J. Polym. Sci. A Polym. Chem.

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The polymerization of isodecyl acrylate (ISODAC) in vesicles of sodium di‐2‐ethylhexyl phosphate (SEHP) loaded with dyes or tyrosine was achieved. The study of the polymerization rate–conversion curve of the monomer confirmed that this polymerization was different from other polymerizations known, as it was proposed previously. Then the entrapment of hydrophilic molecules such as tyrosine or brilliant cresyl blue and lipophilic molecules such as sudan III into the nanoparticles was evaluated and confirmed by different methods. While tyrosine was not retained, brilliant cresyl blue and sudan III were entrapped inside nanoparticles. The size and the polarity of the molecules to be encapsulated, which are related to their diffusion coefficients and partition coefficients between water and the nanoparticle, seem to be the parameters responsible of the entrapment. © 1998 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 36: 3035–3043, 1998

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Section: Tyrosine Entrapment In Nanoparticlesmentioning

confidence: 99%

Nanoparticles from vesicles polymerization. II. Evaluation of their encapsulation capacity

Poulain

Nakache

1998

J. Polym. Sci. A Polym. Chem.

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“…Among the most promising geometries for intracellular sensing and delivery is the recent advent of silica and polymer nanoshells. 10,18–21,25–36 The nanoshell architecture provides an aqueous interior free of diffusional restrictions and non-covalent interactions with the nanoparticle matrix which may degrade the activity of encapsulated cargo, e.g. enzymes, aptamers, etc.…”

Section: Introductionmentioning

confidence: 99%

“…To overcome this limitation, porous nanoshell architectures have been developed. 10,21,24,25,28,30–33,35,37–41 The resultant thin porous nanoshells function similar to a dialysis membrane where the size exclusion limit is determined by the pore size within the nanoshell surface. Porous silica nanoshells are prepared via selective etching of condensed silica nanoparticles to yield a tunable nanoshell diameter and shell thickness.…”

Section: Introductionmentioning

confidence: 99%

“…Porous silica nanoshells are prepared via selective etching of condensed silica nanoparticles to yield a tunable nanoshell diameter and shell thickness. 29–33,42 A very promising approach for nanoshell synthesis relies upon self-assembly of natural or synthetic materials into a three dimensional spherical nanoshell, 21,26–28,34–36 similar to a liposome, followed by pore formation using pore forming proteins, 27,34,37,38,43 bilayer disrupting compounds 44 or skeletonization 10 . Self-assembly is particularly attractive for encapsulating water-soluble, large molecular weight species that may be damaged during sol-gel polymerization and the subsequent chemical etching.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Pore Sizes and Relative Porosity in Porous Nanoshell Architectures Using Dextran Retention with Single Monomer Resolution and Proton Permeation

et al. 2012

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Unilamellar phospholipid vesicles prepared using the polymerizable lipid bis-sorbylphosphatidylcholine (bis-SorbPC) yield three-dimensional nanoarchitectures that are highly permeable to small molecules. The resulting porous phospholipid nanoshells (PPNs) are potentially useful for a range of biomedical applications including nanosensors and nanodelivery vehicles for cellular assays and manipulations. The uniformity and size distribution of the pores, key properties for sensor design and utilization, has not previously been reported. Fluorophore-assisted carbohydrate electrophoresis (FACE) was utilized to assess the nominal molecular weight cutoff limit (NMCL) of the PPN via analysis of retained dextran with single monomer resolution. The NMCL of PPNs prepared from pure bis-SorbPC was equivalent to a 1800 Da linear dextran, corresponding to a maximum pore diameter of 2.6 nm. Further investigation of PPNs prepared using binary mixtures of bis-SorbPC and dioleylphosphatidylcholine (DOPC) revealed a similar NMCL when the bis-SorbPC content exceeded 30 mol %, whereas different size-dependent permeation was observed below this composition. Below 30 mol % bis-SorbPC, dextran retention provided insufficient mass resolution (162 Da) to observe porosity on the experimental time scale; however, proton permeability showed a marked enhancement for bis-SorbPC ≥ 10 mol %. Combined these data suggest that the NMCL for native pores in bis-SorbPC PPNs results from an inherent property within the lipid assembly that can be partially disrupted by dilution of bis-SorbPC below a critical value for domain formation. Additionally, the analytical method described herein should prove useful for the challenging task of elucidating porosity in a range of three-dimensional nanomaterials.

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“…Phosphatidylcholines with diene groups in the fatty acid chains, and in particular DODPC, have been investigated mainly in order to stabilize bilayer structures by means of polymerization. − Hence, the physicochemical properties of aqueous vesicle systems of DODPC have been studied before and after polymerization to optimize the polymerization conversion, to modify the architecture of the polymerized membranes, and finally, to control the stability and the leakage rate of vesicles. − Despite the intensive and imaginative work on polymerizable amphiphiles, the mesomorphism and consequently the phase behavior of most of the synthesized monomers were only little characterized with the exception of diacetylene lipids, which form tubular structures in the gel state. − …”

Section: Introductionmentioning

confidence: 99%

Hydration-Induced Gel States of the Dienic Lipid 1,2-Bis(2,4-octadecadienoyl)-sn-glycero-3-phosphorylcholine and Their Characterization Using Infrared Spectroscopy

et al. 1997

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The lyotropic phase behavior of the dienic lipid 1,2-bis(2,4-octadecadienoyl)-sn-glycero-3-phosphorylcholine (DODPC) has been investigated by means of IR spectroscopy at 25 °C. Gradual hydration has been realized exposing the lipid to an atmosphere of variable relative humidity (RH). Upon scans of decreasing RH, the liquid crystalline lipid undergoes the chain-freezing transition to a metastable gel state. By storage of the sample at low RH, the gel transforms to a crystalline subgel designated as SGI. Subsequent hydration induces the conversion to a second subgel (SGII). The subgel phases are characterized by the dense packing of the acyl chains as indicated by the correlation field splitting of the CH2 rocking and bending modes. Band shifts of phosphate group vibrations as well as the splitting of the carbonyl stretching mode are correlated with the hydration of the polar region of the bilayer given in terms of the molar ratio of water to lipid. The ν1,3(OH) absorption band of water yields qualitative information about the water−lipid interaction. The drastic sharpening of this band in the SGI phase was attributed to the reduction of water binding sites on the lipid, leading to a more uniform population of water molecules adsorbed onto the lipid headgroup. The external conditions of phase transformation of DODPC were compared with corresponding data of dimyristoylphosphatidylcholine (DMPC) having the same number of subsequent methylene segments in the acyl chains. Apparent differences can be attributed to the influence of the diene groups representing a rigid spacer inserted between the methylene chains and the ester groups of the lipid, i.e., in a position near the polar/apolar interface of the bilayer.

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Phase separation of polymerized mixed liposomes: analysis of release behavior of entrapped molecules with skeletonization

Cited by 8 publications

References 13 publications

Nanoparticles from vesicles polymerization. II. Evaluation of their encapsulation capacity

Nanoparticles from vesicles polymerization. II. Evaluation of their encapsulation capacity

Determination of Pore Sizes and Relative Porosity in Porous Nanoshell Architectures Using Dextran Retention with Single Monomer Resolution and Proton Permeation

Hydration-Induced Gel States of the Dienic Lipid 1,2-Bis(2,4-octadecadienoyl)-sn-glycero-3-phosphorylcholine and Their Characterization Using Infrared Spectroscopy

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