Phase Separation Behavior and System Properties of Aqueous Two-Phase Systems with Polyethylene Glycol and Different Salts: Experiment and Correlation

Yuan, Haihua; Liu, Yang; Wei, Wanqian; Zhao, Yongjie

doi:10.1155/2015/682476

Cited by 16 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phase separation might require time because the molecules need to achieve a threshold extent of intermolecular interactions to assemble for the formation of droplets . Several studies have indeed reported LLPS after a certain period of incubation time for a diverse set of proteins, including α-Syn. ,,− Notably, near the binodal LLPS boundary, the protein concentration may be just above the critical concentration but not high enough to induce LLPS immediately . In this region, the droplet assembly is delayed and the droplets formed in this region need to reach a threshold size to stabilize and grow further .…”

Section: Discussionmentioning

confidence: 99%

Modulating α-Synuclein Liquid–Liquid Phase Separation

et al. 2021

View full text Add to dashboard Cite

Liquid–liquid phase separation (LLPS) is a crucial phenomenon for the formation of functional membraneless organelles. However, LLPS is also responsible for protein aggregation in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer’s disease, and Parkinson’s disease (PD). Recently, several reports, including ours, have shown that α-synuclein (α-Syn) undergoes LLPS and a subsequent liquid-to-solid phase transition, which leads to amyloid fibril formation. However, how the environmental (and experimental) parameters modulate the α-Syn LLPS remains elusive. Here, we show that in vitro α-Syn LLPS is strongly dependent on the presence of salts, which allows charge neutralization at both terminal segments of protein and therefore promotes hydrophobic interactions supportive for LLPS. Using various purification methods and experimental conditions, we showed, depending upon conditions, α-Syn undergoes either spontaneous (instantaneous) or delayed LLPS. Furthermore, we delineate that the kinetics of liquid droplet formation (i.e., the critical concentration and critical time) is relative and can be modulated by the salt/counterion concentration, pH, presence of surface, PD-associated multivalent cations, and N-terminal acetylation, which are all known to regulate α-Syn aggregation in vitro. Together, our observations suggest that α-Syn LLPS and subsequent liquid-to-solid phase transition could be pathological, which can be triggered only under disease-associated conditions (high critical concentration and/or conditions promoting α-Syn self-assembly). This study will significantly improve our understanding of the molecular mechanisms of α-Syn LLPS and the liquid-to-solid transition.

show abstract

Section: Discussionmentioning

confidence: 99%

Modulating α-Synuclein Liquid–Liquid Phase Separation

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The comparison of the obtained data in Fig. 3 with the results of Yuan and co-workers 45 , Mobalegholeslam and Bakhshi 46 in the aqueous two phase system (ammonium sulfate and PEG) without cobalt ions and with protein reported by Dumetz and co-workers 47 is shown in Fig. 4 .…”

Section: Resultsmentioning

confidence: 67%

An efficiency strategy for cobalt recovery from simulated wastewater by biphasic system with polyethylene glycol and ammonium sulfate

Amjad

Asadollahzadeh

Torkaman

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Today, biphasic aqueous systems have received more attention than conventional separation methods due to their advantages, such as biocompatibility, low cost, and easy operation. The extraction of cobalt ions from the aqueous phase with the absence and the presence of other ions was investigated using polyethylene glycol, and ammonium sulfate salt without using an extractant. The efficiency was evaluated using operating parameters such as aqueous pH, salt and polymer concentrations, phase volume ratio, and initial metal concentration. The higher temperature, and the lower aqueous pH showed a maximum transfer rate for cobalt ions into the PEG1000 phase. Extraction efficiency under optimal conditions equal to 50% (w/w) polyethylene glycol 1000, 4 M ammonium sulfate, aqueous pH = 2, and 15 min extraction time was over 98%. Results from infrared spectroscopy, and thermo-gravimetric analysis illustrated the presence of the PEG-cobalt ion complex. The observation demonstrated that the biphasic system is the proper technology for wastewater purification.

show abstract

“…Adding to this, citrate is a biodegradable and non-toxic anion, making this molecule more environmentally friendly than other salts used for ATPS. 14,42 As can be seen in Fig. 3 and Table 2, the partition of proteins in PEG 6000-citrate ATPS was slightly different between CMN and CAT venoms.…”

Section: Partition Of Clarified Venomsmentioning

confidence: 80%

“…Therefore, is more likely that proteins migrate towards the citrate‐rich phase in comparison to the phosphate‐rich phase. Adding to this, citrate is a biodegradable and non‐toxic anion, making this molecule more environmentally friendly than other salts used for ATPS 14,42 …”

Section: Resultsmentioning

confidence: 99%

Primary recovery strategies of low‐molecular‐weight toxins from Crotalus molossus nigrescens and Crotalus atrox using aqueous two‐phase and three‐phase partition systems

Sánchez‐Trasviña,

Melendez‐Martínez,

González‐Castañón

et al. 2024

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUNDRattlesnake venoms are a complex source of different toxin families with biotechnological and biomedical applications. However, the complexity of the sample and the low abundance of some toxins can restrict their study and applications. Toxin isolation usually requires multiple chromatographic steps, implying elevated operative costs and processing times. This raises the need for recovery and enrichment of those proteins and peptides. For that reason, we tested two liquid–liquid separation methods – aqueous two‐phase systems (ATPS) and three‐phase partition (TPP) – since these two alternatives have shown great potential for the primary recovery of different toxin families from rattlesnake venoms.RESULTSPolyethylene glycol (PEG)–citrate and ionic liquid (IL)–potassium phosphate ATPS and TPP based on t‐butanol and ammonium sulfate were used to partition different toxin families from Crotalus molossus nigrescens and C. atrox venoms. PEG–citrate allowed a selective partition of proteins between both phases. The best enrichment case was observed with a myotoxin band (6.6 kDa) in the PEG‐rich phase from 2% to 41.3% of abundance. In IL–potassium phosphate systems, proteins showed a preference towards the IL‐rich phase with no selective partition. In TPP, proteins were partitioned mainly to the bottom phase and interphase, but serine proteases (29.1 kDa) were enriched from 3.7% to 56.6% in the top phase.CONCLUSIONThe present study demonstrated that PEG–citrate ATPS and TPP separation methods can generate a selective partition of some toxin families from rattlesnake venoms. These enrichment methods will facilitate the isolation of toxins, from animal venoms, that are relevant for biotechnological and biomedical applications. © 2024 Society of Chemical Industry (SCI).

show abstract

Phase Separation Behavior and System Properties of Aqueous Two-Phase Systems with Polyethylene Glycol and Different Salts: Experiment and Correlation

Cited by 16 publications

References 32 publications

Modulating α-Synuclein Liquid–Liquid Phase Separation

Modulating α-Synuclein Liquid–Liquid Phase Separation

An efficiency strategy for cobalt recovery from simulated wastewater by biphasic system with polyethylene glycol and ammonium sulfate

Primary recovery strategies of low‐molecular‐weight toxins from Crotalus molossus nigrescens and Crotalus atrox using aqueous two‐phase and three‐phase partition systems

Contact Info

Product

Resources

About