Phase-selective staining of model and cell membranes, lipid droplets and lipoproteins with fluorescent solvatochromic pyrene probes

Sot, Jesús; Esnal, Ixone; Monasterio, Bingen G.; León-Irra, Rocío; Niko, Yosuke; Goñi, Félix M.; Klymchenko, Andrey S.; Alonso, Alicia

doi:10.1016/j.bbamem.2020.183470

Cited by 12 publications

(15 citation statements)

References 31 publications

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“…Results regarding the use of PA probes for blood plasma might be interesting. While the use of PA is well documented for membranes and the RBIR values are indicative of bilayer order [29,30], blood plasma does not exhibit a significant lipid organization as there are no cellular elements present, with the exception of platelets. Considering that lipid quantities from platelets are not sufficient for an adequate PA signal, additional experiments were required for a better understanding of PA behavior.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the red/blue intensity ratio (RBIR) is generally a marker of fluidity. Although PA has been reportedly used for membranes [29,30], we used this probe to analyze blood plasma and the results are shown in Figure 7. PA red/blue ratios in the range 0.32-0.38 are commonly found in liquid-ordered bilayers, as they exist in erythrocytes [30].…”

Section: Differences In Blood Plasma Between Obese Patients and Contr...mentioning

confidence: 99%

“…Although PA has been reportedly used for membranes [29,30], we used this probe to analyze blood plasma and the results are shown in Figure 7. PA red/blue ratios in the range 0.32-0.38 are commonly found in liquid-ordered bilayers, as they exist in erythrocytes [30]. A significant decrease in RBIR was detected for obese patient blood plasma.…”

Section: Differences In Blood Plasma Between Obese Patients and Contr...mentioning

confidence: 99%

“…The fluorescence spectra of the PA probe were measured on a QuantaMaster 40 spectrofluorometer (Photon Technology International, Lawrenceville, NJ, USA), with a previously described method [30]. Emission spectra were collected between 450 and 700 nm, exciting at 430 nm for PA, using a 430 nm bandpass filter to minimize detection of dispersed light.…”

Section: Pa Ratio In Blood Plasma Using Fluorescence Spectroscopymentioning

confidence: 99%

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Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients

Sot

García-Arribas

Abad

et al. 2022

IJMS

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This work intends to describe the physical properties of red blood cell (RBC) membranes in obese adults. The hypothesis driving this research is that obesity, in addition to increasing the amount of body fat, will also modify the lipid composition of membranes in cells other than adipocytes. Forty-nine control volunteers (16 male, 33 female, BMI 21.8 ± 5.6 and 21.5 ±4.2 kg/m2, respectively) and 52 obese subjects (16 male and 36 female, BMI 38.2±11.0 and 40.7±8.7 kg/m2, respectively) were examined. The two physical techniques applied were atomic force microscopy (AFM) in the force spectroscopy mode, which allows the micromechanical measurement of penetration forces, and fluorescence anisotropy of trimethylammonium diphenylhexatriene (TMA-DPH), which provides information on lipid order at the membrane polar–nonpolar interface. These techniques, in combination with lipidomic studies, revealed a decreased rigidity in the interfacial region of the RBC membranes of obese as compared to control patients, related to parallel changes in lipid composition. Lipidomic data show an increase in the cholesterol/phospholipid mole ratio and a decrease in sphingomyelin contents in obese membranes. ω-3 fatty acids (e.g., docosahexaenoic acid) appear to be less prevalent in obese patient RBCs, and this is the case for both the global fatty acid distribution and for the individual major lipids in the membrane phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS). Moreover, some ω-6 fatty acids (e.g., arachidonic acid) are increased in obese patient RBCs. The switch from ω-3 to ω-6 lipids in obese subjects could be a major factor explaining the higher interfacial fluidity in obese patient RBC membranes.

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Section: Discussionmentioning

confidence: 99%

Section: Differences In Blood Plasma Between Obese Patients and Contr...mentioning

confidence: 99%

Section: Differences In Blood Plasma Between Obese Patients and Contr...mentioning

confidence: 99%

Section: Pa Ratio In Blood Plasma Using Fluorescence Spectroscopymentioning

confidence: 99%

See 2 more Smart Citations

Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients

Sot

García-Arribas

Abad

et al. 2022

IJMS

Self Cite

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“…However, the field is developing rapidly thanks to an increased interest in the cell biology of lipids, ingenuity and technical advance. On one hand, recent years have brought intense developments in high/super-resolution imaging of cellular membranes, in specific fluorescent probes and reporters of membrane polarity or fluidity/disorder ( Sot et al, 2021 ) and in novel electron microscopy techniques ( Heberle et al, 2020 ). On the other hand, in vitro reconstitution and structural studies have greatly improved our understanding of LTP function at MCS and of elementary but previously elusive PS movements ( Timcenko et al, 2019 ; de la Mora et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Transport Pathways That Contribute to the Cellular Distribution of Phosphatidylserine

Lenoir

D’Ambrosio

Dieudonné

et al. 2021

Front. Cell Dev. Biol.

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Phosphatidylserine (PS) is a negatively charged phospholipid that displays a highly uneven distribution within cellular membranes, essential for establishment of cell polarity and other processes. In this review, we discuss how combined action of PS biosynthesis enzymes in the endoplasmic reticulum (ER), lipid transfer proteins (LTPs) acting within membrane contact sites (MCS) between the ER and other compartments, and lipid flippases and scramblases that mediate PS flip-flop between membrane leaflets controls the cellular distribution of PS. Enrichment of PS in specific compartments, in particular in the cytosolic leaflet of the plasma membrane (PM), requires input of energy, which can be supplied in the form of ATP or by phosphoinositides. Conversely, coupling between PS synthesis or degradation, PS flip-flop and PS transfer may enable PS transfer by passive flow. Such scenario is best documented by recent work on the formation of autophagosomes. The existence of lateral PS nanodomains, which is well-documented in the case of the PM and postulated for other compartments, can change the steepness or direction of PS gradients between compartments. Improvements in cellular imaging of lipids and membranes, lipidomic analysis of complex cellular samples, reconstitution of cellular lipid transport reactions and high-resolution structural data have greatly increased our understanding of cellular PS homeostasis. Our review also highlights how budding yeast has been instrumental for our understanding of the organization and transport of PS in cells.

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Intracellular Physical Properties with Small Organic Fluorescent Probes: Recent Advances and Future Perspectives

Dutta

Pal

Koner

2022

The Chemical Record

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The intracellular physical parameters i. e., polarity, viscosity, fluidity, tension, potential, and temperature of a live cell are the hallmark of cellular health and have garnered immense interest over the past decade. In this context, small molecule organic fluorophores exhibit prominent useful properties including easy functionalizability, environmental sensitivity, biocompatibility, and fast yet efficient cellular uptakability which has made them a popular tool to understand intra‐cellular micro‐environmental properties. Throughout this discussion, we have outlined the basic design strategies of small molecules for specific organelle targeting and quantification of physical properties. The values of these parameters are indicative of cellular homeostasis and subtle alteration may be considered as the onset of disease. We believe this comprehensive review will facilitate the development of potential future probes for superior insight into the physical parameters that are yet to be quantified.

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Phase-selective staining of model and cell membranes, lipid droplets and lipoproteins with fluorescent solvatochromic pyrene probes

Cited by 12 publications

References 31 publications

Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients

Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients

Transport Pathways That Contribute to the Cellular Distribution of Phosphatidylserine

Intracellular Physical Properties with Small Organic Fluorescent Probes: Recent Advances and Future Perspectives

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