Phase retrieval in digital holographic microscopy using a Gerchberg-Saxton algorithm

Cruz, María-Luisa; Castro, Albertina; Arrizón, Víctor

doi:10.1117/12.793894

Cited by 5 publications

(6 citation statements)

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“…The GS algorithm implemented in this paper has been well studied for previously in the context of digital holographic microscopy [19][20][21]. Seeing as the physical process behind the DLHM is similar to that of traditional DHM, the efficacy of the GS algorithm maintained its validity just as previous utilizations have [19][20][21].…”

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confidence: 79%

Seeing cells without a lens: Compact 3D digital lensless holographic microscopy for wide-field imaging

Yang,

2023

TNS

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Optical microscopy is an essential tool for biomedical discoveries and cell diagnosis at micro- to nano-scales. However, conventional microscopes rely on lenses to record 2-D images of samples, which limits in-depth inspection of large volumes of cells. This research project implements a novel 3-D lensless microscopic imaging system that achieves a wide field of view, high resolution, and an extremely compact, cost-effective design: the Digital Lensless Holographic Microscope (DLHM).A lensless holographic microscope is built with only a light source, a sample, and an imaging chip (with other non-essential supporting structures). The entire setup costs $500 to $600. A series of MATLAB-based algorithms were designed to reconstruct phase information of samples simultaneously from the recorded hologram with built-in high-resolution and phase unwrapping functions. This produces 3-D images of cell samples. The 3-D cell reconstruction of biological samples maintained a comparable resolution with conventional optical microscopes while covering a field of view of 36.2 mm2, which is 20-30 times larger. While most microscopes are extremely time-consuming and require professional expertise, the lensless holographic microscope is portable, low-cost, high-stability, and extremely simple. This makes it accessible for point-of-care testing (POCT) to a broader coverage, including developing regions with limited medical facilities.

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confidence: 79%

Seeing cells without a lens: Compact 3D digital lensless holographic microscopy for wide-field imaging

Yang,

2023

TNS

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“…5has been presented for example in Refs. [18,19], where both the object and reference intensity were measured for further subtraction, thus requiring three images for reconstruction.…”

Section: Iterative Methods Principlementioning

confidence: 99%

“…Different methods were proposed to suppress the zero-order term in off-axis holography. A first method based on multiple measurements uses shutters to directly measure the intensity of the two beams interfering for direct subtraction [18,19]. Intensity terms were considered as constant, making it possible to subtract them with a constant term [20]; then, linear filtering was also used with high-pass filters [21,22].…”

Section: Introductionmentioning

confidence: 99%

“…Iterative methods were employed in particular in phase retrieval algorithms [26], and in particular in combination with Fourier interferometry for suppression of the conjugated imaging order [19]. Those methods however can be used for phase-only objects, as this type of algorithms is usually employed for phase recovery from intensity measurements, thus not providing the full complex field.…”

Section: Introductionmentioning

confidence: 99%

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Iterative method for zero-order suppression in off-axis digital holography

Pavillon¹,

Arfire²,

Bergoënd³

et al. 2010

Opt. Express

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We propose a method to suppress the so-called zero-order term in a hologram, based on an iterative principle. During the hologram acquisition process, the encoded information includes the intensities of the two beams creating the interference pattern, which do not contain information about the recorded complex wavefront, and that can disrupt the reconstructed signal. The proposed method selectively suppresses the zero-order term by employing the information obtained during wavefront reconstruction in an iterative procedure, thus enabling its suppression without any a priori knowledge about the object. The method is analyzed analytically and its convergence is studied. Then, its performance is shown experimentally. Its robustness is assessed by applying the procedure on various types of holograms, such as topographic images of microscopic specimens or speckle holograms.

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“…Some DHM setups employ microscope objectives to form the image of the object (Ferraro et al, 2003;Mann et al, 2005;. However, it is also possible to implement DHM in a lensless setup, where the light field scattered from the object is used instead of its projected image (Wagner et al, 1999;Schnars-Juptner, 2002;Repetto et al, 2004;Cruz et al, 2008;Oh et al, 2010). A conventional setup in DHM is based on the use of an external reference wave (RW), which interferes with the field generated by the object, forming an intensity pattern which is known as hologram of the field (Takeda et al, 1982;Kreis, 1986;Yamaguchi-Zhang, 1997;Yamaguchi et al, 2001;Arrizón-Sanchez, 2004;Liebling, 2004;Quian, 2006;Meneses-Fabian, 2006;Guo, 2007;Cruz et al, 2009).…”

Section: Introductionmentioning

confidence: 99%