Phase retrieval approach for coherent anti-Stokes Raman scattering spectrum analysis

Vartiainen, Erik M.

doi:10.1364/josab.9.001209

Cited by 114 publications

(99 citation statements)

References 20 publications

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“…To analyze the CARS signal and retrieve the Raman spectra, we used the approach described in detail by Vartianen et al (21,22). All spectra were collected in transmission geometry with the best-available spectral resolution and with the maximum number of data points recorded (typically, 2,048 for the visible and 4,096 for the near-IR CARS spectra).…”

Section: Experimental Set-up and Methodsmentioning

confidence: 99%

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Comparison of coherent and spontaneous Raman microspectroscopies for noninvasive detection of single bacterial endospores

Petrov

Arora

Yakovlev

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

132

104

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Single bacterial spores were analyzed by using nonlinear Raman microspectroscopy based on coherent anti-Stokes Raman scattering (CARS). The Raman spectra were retrieved from CARS spectra and found to be in excellent agreement with conventionally collected Raman spectra. The phase retrieval method based on maximum entropy model revealed significant robustness to external noise. The direct comparison of signal amplitudes exhibited a factor of 100 stronger CARS signal, as compared with the Raman signal.microscopy ͉ nonlinear optics ͉ scattering stimulated ͉ ultrafast optics

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Section: Experimental Set-up and Methodsmentioning

confidence: 99%

“…The spectra were retrieved by using the maximum entropy method (21,22), and the results were compared with the Raman data for those endospores (2,3).…”

mentioning

confidence: 99%

Comparison of coherent and spontaneous Raman microspectroscopies for noninvasive detection of single bacterial endospores

Petrov

Arora

Yakovlev

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

132

104

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“…With the full power of the incident beams on a sample (0.1 J/cm 2 , which is considered to be safe for this pulse duration and wavelength range 32 ), we typically achieved a full saturation of the CCD within an acquisition time as short as 10 ms. All the collected CARS spectra were normalized to the reference CARS spectrum collected from a nonresonant medium (either fused silica window or double distilled water solution), and Raman spectra were retrieved using an algorithm, which is described elsewhere. 7,8,33 In brief, we assumed that the nonresonant tensor component, χ (3) N R , is real and frequency independent and used a maximum entropy method 33 to extract the imaginary part of the resonant tensor component, χ…”

Section: Journal Of Biomedical Opticsmentioning

confidence: 99%

Hyperspectral coherent anti-Stokes Raman scattering microscopy imaging through turbid medium

2011

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Abstract. Coherent Raman microspectroscopy imaging is an emerging technique for noninvasive, chemically specific optical imaging, which can be potentially used to analyze the chemical composition and its distribution in biological tissues. In this report, a hierarchical cluster analysis was applied to hyperspectral coherent anti-Stokes Raman imaging of different chemical species through a turbid medium. It was demonstrated that by using readily available commercial software (Cytospec, Inc.) and cluster analysis, distinct chemical species can be imaged and identified through a rather thick layer of scattering medium. Once the clusters of different chemical composition were distinguished, a phase retrieval algorithm was used to convert coherent anti-Stokes Raman spectra to Raman spectra, which were used for chemical identification of hidden microscopic objects. In particular, applications to remote optical sensing of potential biological threats and to imaging through a layer of skin tissue were successfully demonstrated. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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“…2͑c͔͒. This procedure yields the imaginary part of the third-order nonlinear susceptibility, Im ͓ ͑3͒ ͔, which can be shown 16 to be identical to the Raman lineshape if both CARS and Raman spectra are obtained using all-parallel polarization conditions. Comparison of the Raman spectra ͑Fig.…”

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confidence: 99%

Spectroscopic analysis of the oxygenation state of hemoglobin using coherent anti-Stokes Raman scattering

Rinia¹,

Bonn

Vartiainen

et al. 2006

J. Biomed. Opt.

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Abstract.A method for noninvasively determining blood oxygenation in individual vessels inside bulk tissue would provide a powerful tool for biomedical research. We explore the potential of coherent anti-Stokes Raman scattering ͑CARS͒ spectroscopy to provide this capability. Using the multiplex CARS approach, we measure the vibrational spectrum in hemoglobin solutions as a function of the oxygenation state and observe a clear dependence of the spectral shape on oxygenation. The direct extraction of the Raman line shape from the CARS data using a maximum entropy method phase retrieval algorithm enables quantitative analysis. The CARS spectra associated with intermediate oxygenation saturation levels can be accurately described by a weighted sum of the fully oxygenated and fully deoxygenated spectra. We find that the degree of oxygenation determined from the CARS data agrees well with that determined by optical absorption. As a nonlinear optical technique, CARS inherently provides the 3-D imaging capability and tolerance to scattering necessary for biomedical applications. We discuss the challenges in extending the proof of principle demonstrated to in vivo applications.

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Phase retrieval approach for coherent anti-Stokes Raman scattering spectrum analysis

Cited by 114 publications

References 20 publications

Comparison of coherent and spontaneous Raman microspectroscopies for noninvasive detection of single bacterial endospores

Comparison of coherent and spontaneous Raman microspectroscopies for noninvasive detection of single bacterial endospores

Hyperspectral coherent anti-Stokes Raman scattering microscopy imaging through turbid medium

Spectroscopic analysis of the oxygenation state of hemoglobin using coherent anti-Stokes Raman scattering

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