Phase microscopy using light-field reconstruction method for cell observation

Xiu, Peng; Zhou, Xin; Kuang, Cuifang; Xu, Yingke; Liu, Xu

doi:10.1016/j.micron.2015.04.009

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…Following, we introduced four-step PSI, a high accuracy phase measurement technique, to perform phase retrieval of single cell 22, 23 , in which four-frame phase-shifting interferograms I 1 ( x , y ), I 2 ( x , y ), I 3 ( x , y ) and I 4 ( x , y ) with the phase shifts of 0, π/2, π, 3π/2 were respectively captured by a CCD, which was executed by the control system, thus the quantitative phase of measured cell can be expressed as …”

Section: Methodsmentioning

confidence: 99%

Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging

Zhang

Zhong

Tang

et al. 2017

Sci Rep

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Cell refractive index, an intrinsic optical parameter, is closely correlated with the intracellular mass and concentration. By combining optical phase-shifting interferometry (PSI) and atomic force microscope (AFM) imaging, we constructed a label free, non-invasive and quantitative refractive index of single cell measurement system, in which the accurate phase map of single cell was retrieved with PSI technique and the cell morphology with nanoscale resolution was achieved with AFM imaging. Based on the proposed AFM/PSI system, we achieved quantitative refractive index distributions of single red blood cell and Jurkat cell, respectively. Further, the quantitative change of refractive index distribution during Daunorubicin (DNR)-induced Jurkat cell apoptosis was presented, and then the content changes of intracellular biochemical components were achieved. Importantly, these results were consistent with Raman spectral analysis, indicating that the proposed PSI/AFM based refractive index system is likely to become a useful tool for intracellular biochemical components analysis measurement, and this will facilitate its application for revealing cell structure and pathological state from a new perspective.

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Section: Methodsmentioning

confidence: 99%

Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging

Zhang

Zhong

Tang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

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Analogous on‐axis interference topographic phase microscopy (AOITPM)

Xiu

Liu

Zhou

et al. 2018

Journal of Microscopy

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The refractive index (RI) of a sample as an endogenous contrast agent plays an important role in transparent live cell imaging. In tomographic phase microscopy (TPM), 3D quantitative RI maps can be reconstructed based on the measured projections of the RI in multiple directions. The resolution of the RI maps not only depends on the numerical aperture of the employed objective lens, but also is determined by the accuracy of the quantitative phase of the sample measured at multiple scanning illumination angles. This paper reports an analogous on-axis interference TPM, where the interference angle between the sample and reference beams is kept constant for projections in multiple directions to improve the accuracy of the phase maps and the resolution of RI tomograms. The system has been validated with both silica beads and red blood cells. Compared with conventional TPM, the proposed system acquires quantitative RI maps with higher resolution (420 nm @λ = 633 nm) and signal-to-noise ratio that can be beneficial for live cell imaging in biomedical applications.

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Phase microscopy using light-field reconstruction method for cell observation

Cited by 2 publications

References 21 publications

Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging

Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging

Analogous on‐axis interference topographic phase microscopy (AOITPM)

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