Phase imaging microscopy for the diagnostics of plasma-cell interaction

Ohene, Yolanda; Marinov, Ilya; Laulanié, Lucie de; Dupuy, Corinne; Wattelier, Benoit; Starikovskaia, Svetlana

doi:10.1063/1.4922525

Cited by 6 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another important reason for the stronger anti-tumor effect of direct CAP treatment could be physical. Next to RONS production, CAP also generates thermal-and ultraviolet (UV) radiation, which also have a strong impact on tumor cell behavior [54][55][56], and which are not available in the case of indirect treatment with PTS.…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma Changes the Amino Acid Composition of Solutions and Influences the Anti-Tumor Effect on Melanoma Cells

Arndt

Fadil

Dettmer

et al. 2021

IJMS

View full text Add to dashboard Cite

Cold Atmospheric Plasma (CAP) is an ionized gas near room temperature. Its anti-tumor effect can be transmitted either by direct treatment or mediated by a plasma-treated solution (PTS), such as treated standard cell culture medium, which contains different amino acids, inorganic salts, vitamins and other substances. Despite extensive research, the active components in PTS and its molecular or cellular mechanisms are not yet fully understood. The purpose of this study was the measurement of the reactive species in PTS and their effect on tumor cells using different plasma modes and treatment durations. The PTS analysis yielded mode- and dose-dependent differences in the production of reactive oxygen and nitrogen species (RONS), and in the decomposition and modification of the amino acids Tyrosine (Tyr) and Tryptophan (Trp). The Trp metabolites Formylkynurenine (FKyn) and Kynurenine (Kyn) were produced in PTS with the 4 kHz (oxygen) mode, inducing apoptosis in Mel Im melanoma cells. Nitrated derivatives of Trp and Tyr were formed in the 8 kHz (nitrogen) mode, elevating the p16 mRNA expression and senescence-associated ß-Galactosidase staining. In conclusion, the plasma mode has a strong impact on the composition of the active components in PTS and affects its anti-tumor mechanism. These findings are of decisive importance for the development of plasma devices and the effectiveness of tumor treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma Changes the Amino Acid Composition of Solutions and Influences the Anti-Tumor Effect on Melanoma Cells

Arndt

Fadil

Dettmer

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…263 "Real-time" QPI measurements of blood samples have been demonstrated, utilizing parallel computing strategies to calculate diagnostically relevant cell parameters without storing phase images, allowing for smaller electronic storage and data transmission requirements, which could benefit remote diagnoses of RBC diseases. 264 QPI has also been used to measure morphology changes in HTori thyroid cells during treatment with plasma from a nanosecond dielectric barrier discharge, 265 changes in macrophages from chemically induced apoptosis and dynamic phagocytosis, 201 and for sperm selection for bovine in vitro fertilization. 266 SLIM and tissue spatial correlation analysis was used to assess breast cancer fixed tissue microarrays and showed a 94% sensitivity and 85% specificity for cancer detection 267 (Figure 5d), independent of tissue staining quality.…”

Section: Qpi Applications In Screening and Drug Sensitivity Measurementmentioning

confidence: 99%

Quantitative Phase Imaging: Recent Advances and Expanding Potential in Biomedicine

et al. 2022

View full text Add to dashboard Cite

Quantitative phase imaging (QPI) is a label-free, wide-field microscopy approach with significant opportunities for biomedical applications. QPI uses the natural phase shift of light as it passes through a transparent object, such as a mammalian cell, to quantify biomass distribution and spatial and temporal changes in biomass. Reported in cell studies more than 60 years ago, ongoing advances in QPI hardware and software are leading to numerous applications in biology, with a dramatic expansion in utility over the past two decades. Today, investigations of cell size, morphology, behavior, cellular viscoelasticity, drug efficacy, biomass accumulation and turnover, and transport mechanics are supporting studies of development, physiology, neural activity, cancer, and additional physiological processes and diseases. Here, we review the field of QPI in biology starting with underlying principles, followed by a discussion of technical approaches currently available or being developed, and end with an examination of the breadth of applications in use or under development. We comment on strengths and shortcomings for the deployment of QPI in key biomedical contexts and conclude with emerging challenges and opportunities based on combining QPI with other methodologies that expand the scope and utility of QPI even further.

show abstract

“…In 2015, Ohene et al observed the benefit of the high contrast of CGM to monitor the deformation and condensation of living cells during apoptosis . The study mainly benefited from the good contrast of CGM, not from the quantitative nature of the measurements.…”

Section: Historical Review Of the Applicationsmentioning

confidence: 99%

Wavefront Microscopy Using Quadriwave Lateral Shearing Interferometry: From Bioimaging to Nanophotonics

Baffou

2023

ACS Photonics

View full text Add to dashboard Cite

Common cameras are only sensitive to the intensity of light, discarding an essential feature of a light wave: its phase profile or, equivalently, its wavefront profile. This Review focuses on a rising wavefront imaging technique called quadriwave lateral shearing interferometry (QLSI), based on the simple use of a 2dimensional diffraction grating, aka a cross-grating, in front of a regular camera. We detail the working principle of QLSI and its implementation on an optical microscope. We highlight its microscopy applications in bioimaging and nanophotonics, in particular for the characterization of living cells, nanoparticles, 2D materials, metasurfaces, microscale temperature gradients, and surface topography. Finally, we draw a critical comparison of QLSI with current quantitative phase microscopy techniques, namely, digital holography microscopy (DHM), spatial light interference microscopy (SLIM), and diffraction phase microscopy (DPM).

show abstract

Phase imaging microscopy for the diagnostics of plasma-cell interaction

Abstract: Single beam Fourier transform digital holographic quantitative phase microscopy Appl. Phys. Lett.

Cited by 6 publications

References 21 publications

Cold Atmospheric Plasma Changes the Amino Acid Composition of Solutions and Influences the Anti-Tumor Effect on Melanoma Cells

Cold Atmospheric Plasma Changes the Amino Acid Composition of Solutions and Influences the Anti-Tumor Effect on Melanoma Cells

Quantitative Phase Imaging: Recent Advances and Expanding Potential in Biomedicine

Wavefront Microscopy Using Quadriwave Lateral Shearing Interferometry: From Bioimaging to Nanophotonics

Contact Info

Product

Resources

About