Phase function measurements on nonspherical scatterers using a two-axis goniometer

Forster, Florian K.; Kienle, Alwin; Michels, René; Hibst, Raimund

doi:10.1117/1.2187421

Cited by 22 publications

(7 citation statements)

References 24 publications

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“…44 In the present case, the orientation of the dentin tubules parallel to the light application favors the transmission of the radiation and thus increases the luminous efficiency. [45][46][47][48] Nevertheless, the layer thickness remains a strongly limiting factor of transmission. As could be observed in this study, dentin (500 µm) attenuated the laser power by approximately 30%, whereas a thickness of 1000 µm reduced the power to only a third.…”

Section: Investigation Of Light Transmission: Influencing Variablesmentioning

confidence: 99%

Cavity Disinfection With a 445 nm Diode Laser Within the Scope of Restorative Therapy – A Pilot Study

et al. 2020

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Introduction: Cavity disinfection is necessary to prevent a progressive infection of the crown dentin and pulp. Increasing intolerance and resistance to antiseptics and antibiotics as well as the controversy over the effects of those on the dental hard tissue and composite have prompted the investigation of alternative treatment options. The objective of this pilot study is to evaluate the antibacterial potential of a diode laser with a wavelength of 445 nm in the cavity preparation using the bacterium Streptococcus salivarius associated with caries in conjunction with the characteristics and influences of dentin on light transmission. Methods: The bactericidal effect of the laser irradiation was determined in culture experiments by using caries-free human dentin samples on bacteria-inoculated agar. For this, dentin discs (horizontally cut coronal dentin) of 500 µm and 1000 µm thicknesses were produced and irradiated with the laser with irradiation parameters of 0.7-1 W in a cw-mode and exposure times of between 5-30 s. Based on the different sample thicknesses, the penetration depth effect of the irradiation was ascertained after the subsequent incubation of the bacteria-inoculated agar. Additional influential parameters on the irradiation transmission were investigated, including surface moisture, tooth color as well as the presence of a smear layer on the dentin surface. Results:The optical transmission values of the laser radiation for dentin were significantly dependent on the sample thickness (P = 0.006) as well as its moisture content (P = 0.013) and were independent of the presence of a smear layer. There was a 40% reduction in bacteria after the radiography of the 500-µm-thick dentin samples, which was shown as the lowest laser dose (443 J/cm 2 ). Conclusion:These findings indicate that the diode laser with light emission at a wavelength of 445 nm is interesting for the supportive cavity disinfection within the scope of caries therapy and show potential for clinical applications.

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Section: Investigation Of Light Transmission: Influencing Variablesmentioning

confidence: 99%

Cavity Disinfection With a 445 nm Diode Laser Within the Scope of Restorative Therapy – A Pilot Study

et al. 2020

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“… [5,6] Also, aggregation‐caused fluorescence quenching (ACQ) further added to the drawback. The fluorescence behavior of J‐ and H‐aggregates has been theoretically explained through the coupled oscillator model of T. Förster [7] and the molecular exciton theory of M. Kasha [8] . A few exceptions have been reported for H‐aggregates that show fluorescence emission, and their mechanism has been discussed [9–11] .…”

Section: Introductionmentioning

confidence: 99%

Effective Light‐Induced Fluorescent H‐Aggregation of Fluorinated Pyridyl‐Azo Dye via Photoisomerization

Modi,

Patel,

Mahato

et al. 2024

ChemPhotoChem

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Here we present the synthesis of new fluorinated pyridyl‐azo dye and demonstrate an effective way to convert monomer fluorinated pyridyl‐azo dye to H‐aggregated through UV‐light induced trans‐cis‐trans photoisomerization. And upto 12:88 ratio between monomer : aggregation was achieved through a small pulse of UV irradiation. H‐aggregation shows unique high extinction co‐efficient in UV‐visible absorbance and unexpectedly fluorescence emission with remarkably large Stokes shift. The solvent DMF and fluorine substituent were found to have a critical role in aggregation. 1H, 19F NMR and 19F‐DOSY NMR experiments were used to confirm the aggregation and also confirmed the absence of p‐p stacking.

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“…The theory of fluorescence resonance energy transfer (FRET) was first proposed in 1948 as a means to detect interactions between molecules that approach within 10 nm. [ 10 ] Significant progress has been made to develop biological applications of FRET technology, particularly for in vitro detection of molecular interactions. [ 11–15 ] Successful implementation of FRET technology requires a suitable pair of fluorescent systems with overlapping emission and absorption spectra, as well as the capacity to be excited by specified conditions.…”

Section: Introductionmentioning

confidence: 99%

Ion Monitoring at Nanoscale Sites of Interorganelle Membrane Contact in Living Cells

Sun,

Wang,

Zhang

et al. 2023

Small Structures

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Nanostructural contact sites formed by interorganelle membrane contacts, including mitochondria and lysosome contacts (MLC), facilitate the exchange of substances during various life processes. However, existing bioanalytical technologies have yet to provide accurate information on the exchange of substances, as these techniques exhibit limited spatial and temporal resolution. To address this limitation, a strategy is proposed that combines fluorescence resonance energy transfer (FRET) probes with high spatial resolution detection and super‐resolution microscopes (SRM) that feature time‐resolved imaging. Specifically, a proof‐of‐concept approach is presented for monitoring H+ fluctuations during MLC with a spatial H+ biosensor targeting lysosomes, BDP‐RhB. The biosensor comprises H+‐sensitive rhodamine B as a FRET acceptor connected by a flexible chain to a BODIPY derivative as a donor. The acidity of MLC sites may vary, influencing the spatial distance of the flexible chain and causing a fluorescence transition in BDP‐RhB. Consequently, the spatial distribution of H+ can be identified using SRM. Furthermore, an algorithm has been developed to screen and identify potential compounds that control substance exchange in the MLC. Collectively, this work presents the dynamic of H+ in lysosomes within living cells, which provides a drug screening tool for studying substance exchange through interorganelle membrane contacts.

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Phase function measurements on nonspherical scatterers using a two-axis goniometer

Cited by 22 publications

References 24 publications

Cavity Disinfection With a 445 nm Diode Laser Within the Scope of Restorative Therapy – A Pilot Study

Cavity Disinfection With a 445 nm Diode Laser Within the Scope of Restorative Therapy – A Pilot Study

Effective Light‐Induced Fluorescent H‐Aggregation of Fluorinated Pyridyl‐Azo Dye via Photoisomerization

Ion Monitoring at Nanoscale Sites of Interorganelle Membrane Contact in Living Cells

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