Phase diagrams for dextran-PEG aqueous two-phase systems at 22°C

Diamond, Alan D.; Hsu, Jong-Ping

doi:10.1007/bf01875564

Cited by 44 publications

(34 citation statements)

References 3 publications

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“…Therefore, species with low affinity for water are also more able to be phase separated and to form an ATPS. This tendency is in agreement with other experimental results for ATPS composed of polymers and ionic liquids [45], polymers and salts [46] or dextran and a polymer [47].…”

Section: Resultssupporting

confidence: 93%

Aqueous two-phase systems formed by biocompatible and biodegradable polysaccharides and acetonitrile

Cardoso

Souza

Pereira

et al. 2014

Separation and Purification Technology

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In this work, it is shown that novel aqueous two-phase systems can be formed by the combination of acetonitrile and polysaccharides, namely dextran. Several ternary phase diagrams were determined at 25 °C for the systems composed of water + acetonitrile + dextran. The effect of the dextran molecular weight (6,000, 40,000 and 100,000 g.mol −1 ) was ascertained toward their ability to undergo liquid-liquid demixing. An increase in the dextran molecular weight favors the phase separation. Furthermore, the effect of temperature (25, 35 and 45 °C) was evaluated for the system constituted by the dextran of higher molecular weight. Lower temperatures are favorable for phase separation since lower amounts of dextran and acetonitrile are required for the creation of aqueous two-phase systems. In general, acetonitrile is enriched in the top phase while dextran is majorly concentrated in the bottom phase. The applicability of this new type of two-phase systems as liquid-liquid extraction approaches was also evaluated by the study of the partition behavior of a well-known antioxidant -vanillin -and used here as a model biomolecule. The optimized conditions led to an extraction efficiency of vanillin of 95% at the acetonitrile-rich phase.

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Section: Resultssupporting

confidence: 93%

Aqueous two-phase systems formed by biocompatible and biodegradable polysaccharides and acetonitrile

Cardoso

Souza

Pereira

et al. 2014

Separation and Purification Technology

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“…At low glucose concentrations, glucose was added to maintain a 10 mM concentration in the medium. The polymer content of the fresh top phase was based on the composition of the top phase at 22°C, 5.5% (w/w) PEG-5.2% (w/w) DEX (Diamond and Hsu, 1989).…”

Section: Repeated Extractive Batch Fermentationsmentioning

confidence: 99%

Analysis of phase composition in aqueous two-phase systems using a two-column chromatographic method: Application to lactic acid production by extractive fermentation

Planas¹,

Lefebvre²,

Tjerneld³

et al. 1997

Biotechnol. Bioeng.

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“…One of the most widely studied ATPS is the polyethylene glycol (PEG)/dextran system9. Many other ATPSs have been reported including ATPSs that utilize block co-polymers10111213.…”

mentioning

confidence: 99%

“…Aqueous two-phase systems (ATPSs) have been studied over the past decades due to its potential in bioseparation, high throughout assays, microfluidics, diagnostics, and bioreactors 1 2 3 4 5 6 7 8 . One of the most widely studied ATPS is the polyethylene glycol (PEG)/dextran system 9 . Many other ATPSs have been reported including ATPSs that utilize block co-polymers 10 11 12 13 .…”

mentioning

confidence: 99%

Aqueous Two Phase System Assisted Self-Assembled PLGA Microparticles

Yeredla

Kojima

et al. 2016

Sci Rep

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Here, we produce poly(lactide-co-glycolide) (PLGA) based microparticles with varying morphologies, and temperature responsive properties utilizing a Pluronic F127/dextran aqueous two-phase system (ATPS) assisted self-assembly. The PLGA polymer, when emulsified in Pluronic F127/dextran ATPS, forms unique microparticle structures due to ATPS guided-self assembly. Depending on the PLGA concentration, the particles either formed a core-shell or a composite microparticle structure. The microparticles facilitate the simultaneous incorporation of both hydrophobic and hydrophilic molecules, due to their amphiphilic macromolecule composition. Further, due to the lower critical solution temperature (LCST) properties of Pluronic F127, the particles exhibit temperature responsiveness. The ATPS based microparticle formation demonstrated in this study, serves as a novel platform for PLGA/polymer based tunable micro/nano particle and polymersome development. The unique properties may be useful in applications such as theranostics, synthesis of complex structure particles, bioreaction/mineralization at the two-phase interface, and bioseparations.

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Phase diagrams for dextran-PEG aqueous two-phase systems at 22°C

Cited by 44 publications

References 3 publications

Aqueous two-phase systems formed by biocompatible and biodegradable polysaccharides and acetonitrile

Aqueous two-phase systems formed by biocompatible and biodegradable polysaccharides and acetonitrile

Analysis of phase composition in aqueous two-phase systems using a two-column chromatographic method: Application to lactic acid production by extractive fermentation

Aqueous Two Phase System Assisted Self-Assembled PLGA Microparticles

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