Phase contrast enhancement in microscopy using spiral phase filtering

Situ, Guohai; Warber, Michael; Pedrini, Giancarlo; Osten, Wolfgang

doi:10.1016/j.optcom.2009.11.084

Cited by 56 publications

(17 citation statements)

References 19 publications

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“…However, they introduce a variation in the light phase in comparison with the surrounding medium incoming from a slightly higher refractive index value (Allen et al 1995). Thus, classical phase contrast enhancement techniques (Zernike 1942a(Zernike , 1942bHoffman and Gross 1975) and recent developments (Fürhapter et al 2005;Situ et al 2010;Iglesias 2011) have enabled the qualitative visualisation of phase samples. Recently, quantitative characterisation of phase samples has become a reality because of the development of a wide range of techniques based on interferometric principles (Kim 2010;Popescu 2011;Shaked et al 2012;Marquet et al 2014;Majeed et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Effect of counting chamber depth on the accuracy of lensless microscopy for the assessment of boar sperm motility

Soler

Picazo-Bueno

Micó

et al. 2018

Reprod. Fertil. Dev.

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Sperm motility is one of the most significant parameters in the prediction of male fertility. Until now, both motility analysis using an optical microscope and computer-aided sperm analysis (CASA-Mot) entailed the use of counting chambers with a depth to 20µm. Chamber depth significantly affects the intrinsic sperm movement, leading to an artificial motility pattern. For the first time, laser microscopy offers the possibility of avoiding this interference with sperm movement. The aims of the present study were to determine the different motility patterns observed in chambers with depths of 10, 20 and 100µm using a new holographic approach and to compare the results obtained in the 20-µm chamber with those of the laser and optical CASA-Mot systems. The ISAS®3D-Track results showed that values for curvilinear velocity (VCL), straight line velocity, wobble and beat cross frequency were higher for the 100-µm chambers than for the 10- and 20-µm chambers. Only VCL showed a positive correlation between chambers. In addition, Bayesian analysis confirmed that the kinematic parameters observed with the 100-µm chamber were significantly different to those obtained using chambers with depths of 10 and 20µm. When an optical analyser CASA-Mot system was used, all kinematic parameters, except VCL, were higher with ISAS®3D-Track, but were not relevant after Bayesian analysis. Finally, almost three different three-dimensional motility patterns were recognised. In conclusion, the use of the ISAS®3D-Track allows for the analysis of the natural three-dimensional pattern of sperm movement.

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Section: Discussionmentioning

confidence: 99%

Effect of counting chamber depth on the accuracy of lensless microscopy for the assessment of boar sperm motility

Soler

Picazo-Bueno

Micó

et al. 2018

Reprod. Fertil. Dev.

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“…Examples of this effect are shown in figure 4. This approach obviously requires a certain degree of spatial coherence, but relief-like effects with a spiral phase plate have also been demonstrated for incoherent illumination by an LED in a Köhler illumination scheme [27], in order to reduce unwanted coherence artefacts, similar to those discussed before. …”

Section: (C) Pseudo-relief Imagesmentioning

confidence: 93%

Orbital angular momentum light in microscopy

Ritsch‐Marte

2017

Phil. Trans. R. Soc. A.

111

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“…When | | increases, vertex of the triangle would move toward the top right and the effective coding area would increase. In the effective coding area, peaks of the crosstalk term occur where there is discontinuity in the encoded image, and those peaks were used for the edge detection in microscopy [28,29].…”

Section: Encoding 2dmentioning

confidence: 99%

Encoding and Multiplexing of 2D Images with Orbital Angular Momentum Beams and the Use for Multiview Color Displays

Chu

Smithwick

2019

Research

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The orthogonal nature of different orbital angular momentum modes enables information transmission in optical communications with increased bandwidth through mode division multiplexing. So far the related works have been focused on using orbital angular momentum modes to encode/decode and multiplex point-based on-axis signals for maximum data channel numbers and capacity. Whether orbital angular momentum modes can be utilized to encode/decode off-axis signals for multiplexing in two-dimensional space is of significant importance both fundamentally and practically for its enormous potential in increasing the channel information capacity. In this work, a direct use of orbital angular momentum modes to encode/decode and multiplex two-dimensional images is realized in a scalable multiview display architecture, which can be utilized for viewing three-dimensional images from different angles. The effect of off-axis encoding/decoding and the resultant crosstalk between multiplexed different two-dimensional views are studied. Based on which, a color display of good image quality with four independent views is demonstrated. The resolution of the decoded images is analyzed and the limitation of this approach discussed. Moreover, a spatially multiplexed data communication scheme is also proposed with such a two-dimensional encoding/decoding approach to significantly enhance the data transmission capacity in free space for future data communication needs.

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Phase contrast enhancement in microscopy using spiral phase filtering

Cited by 56 publications

References 19 publications

Effect of counting chamber depth on the accuracy of lensless microscopy for the assessment of boar sperm motility

Effect of counting chamber depth on the accuracy of lensless microscopy for the assessment of boar sperm motility

Orbital angular momentum light in microscopy

Encoding and Multiplexing of 2D Images with Orbital Angular Momentum Beams and the Use for Multiview Color Displays

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