Phase change-related variations of dome shape in Eucalyptus urophylla × Eucalyptus grandis shoot apical meristems

Mankessi, François; Saya, Aubin Rachel; Boudon, Frédéric; Guédon, Yann; Montes, Fabienne; Lartaud, Marc; Verdeil, Jean‐Luc; Monteuuis, Olivier

doi:10.1007/s00468-010-0444-7

Cited by 9 publications

(16 citation statements)

References 34 publications

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“…This could account for the higher morphological but also zone measurement and cell number scores recorded for Mat SAMs compared to Juv ones. These observations, in accordance with other studies on various species (Tepper 1963;Monteuuis 1989;Mankessi et al 2010;, suggest that SAM size and associated number of cells could be more influenced by the vigor of the shoot axis underneath than by heteroblasty per se. This view is supported by the fact that in in vitro conditions where such differences in size between shoots with phyllodes and shoot with compound leaves did not exist, IVJ and IVM SAMs looked similar, except at the nucleus level.…”

Section: Influence Of Heteroblasty On Sam Characteristics Outdoorssupporting

confidence: 92%

“…The situation looked different for Juv SAM nuclei where the fewer chromocenters and the lighter staining suggest a higher proportion of decondensed chromatin in the nucleoplasm but also in the nucleolei to regulate nucleolus activities including ribosome biogenesis (Fransz 2008;McKewon and Shaw 2009;Bártová et al 2010). This strengthens the assumption that SAMs from juvenile material are likely transcriptionally more active than SAMs from mature material, consistently with our observations on eucalypts (Mankessi et al 2010;.…”

Section: Influence Of Heteroblasty On Sam Characteristics Outdoorssupporting

confidence: 92%

“…Eucalyptus urophylla x Eucalyptus grandis notwithstanding for this latter case a marked influence of the plastochron (Mankessi et al 2010; mangium only in vitro conditions. These rejuvenations demonstrate that heteroblastic development is liable to be influenced by the environment, and can occasionally include reverse morphological changes i.e.…”

Section: Influence Of Heteroblasty On Sam Characteristics In Vitromentioning

confidence: 79%

“…mangium SAMs, although slightly bigger than in E. urophylla X E. grandis (Mankessi et al 2010) but smaller than for a lot of Gymnosperms and Angiosperms (Clowes 1961), showed the zonation pattern classically depicted in higher plants (Clowes 1961;Romberger 1963;Mankessi et al 2011). Variations of SAM size, shape and even histocytological characteristics have been noticed between and even within various arborescent species (Parke 1959;Owston and Molder 1973;Monteuuis 1989).…”

Section: Sampling Considerationsmentioning

confidence: 90%

“…Variations of SAM size, shape and even histocytological characteristics have been noticed between and even within various arborescent species (Parke 1959;Owston and Molder 1973;Monteuuis 1989). These differences could be caused by variations of organogenic activity or plastochron phase of the SAMs observed, as recently demonstrated in Eucalyptus urophylla x E.ucalyptus grandis (Mankessi et al 2010;. In temperate countries with highly contrasted seasonal variations of photoperiod and temperature, such within origin heterogeneity can be minimized by studying SAMs in similar physiological conditions, for instance resting or budbreak stage (Parke 1959;Owston 1969;Monteuuis 1989).…”

Section: Sampling Considerationsmentioning

confidence: 95%

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Characteristics of Acacia mangium shoot apical meristems in natural and in vitro conditions in relation to heteroblasty

Hatt

Mankessi

Durand

et al. 2012

Trees

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Abstract.The influence of heteroblasty on morphological and histocytological characteristics of Acacia mangium shoot apical meristems (SAMs) was assessed comparing materials with mature and juvenile leaf morphology in natural and in vitro conditions. SAM dome height (H), basal diameter (D) and shape (S) varied significantly according to the four origins of plant material investigated. H and D were highly correlated, with bigger values for the mature source from outdoors "Mat" than for the other origins. Mat SAMs scored also the higher values for the size and the number of cells of the two tunica layers (L1 and L2), the central mother cells (CMC), the peripheral zone (PZ) and the combination of these four SAM zones (4CZ). Overall, cells were noticeably more vacuolized in SAMs from Mat and also from the mature ("IVM") and juvenile-like "IVJ" in vitro origins than in those from the outdoor juvenile source "Juv". These latter were characterized by larger cells and nuclei for L1 and L2 tunica layers, whereas Mat SAMs showed smaller nuclei and nucleoplasmic ratios for L1, L2, CMC and PZ zones. Some Mat SAM nuclei looked densely stained by NBB while others exhibited numerous chromocenters disseminated in the nucleoplasm where nucleolei could hardly be distinguished. By contrast Juv as well as IVJ and IVM SAM nuclei appeared more evenly and lightly stained and could show clearly distinguishable darker nucleolei possibly surrounded by less abundant chromocenters.

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Section: Influence Of Heteroblasty On Sam Characteristics Outdoorssupporting

confidence: 92%

Section: Influence Of Heteroblasty On Sam Characteristics Outdoorssupporting

confidence: 92%

Section: Influence Of Heteroblasty On Sam Characteristics In Vitromentioning

confidence: 79%

Section: Sampling Considerationsmentioning

confidence: 90%

Section: Sampling Considerationsmentioning

confidence: 95%

See 3 more Smart Citations

Characteristics of Acacia mangium shoot apical meristems in natural and in vitro conditions in relation to heteroblasty

Hatt

Mankessi

Durand

et al. 2012

Trees

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Histocytological characteristics of Eucalyptus urophylla × Eucalyptus grandis shoot apical meristems of different physiological ages

Mankessi

Saya

Montes

et al. 2011

Trees

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Histocytological characteristics of Eucalyptus urophylla 9 Eucalyptus grandis shoot apical meristems (SAMs) were described, comparing five outdoor and in vitro sources of akin genotypes differing in their physiological age. The size and the number of cells of the five zones identified within each SAM, i.e. the two tunica layers (L1 and L2), the central mother cells (CMC), the peripheral zone (PZ) and the combination of these four zones (4CZ) varied according to physiological age and plastochron phase. These five zones were significantly larger with higher numbers of cells for SAMs from mature and juvenile trees than for those from physiologically rejuvenated, in vitro mature and in vitro juvenile plants. However, these origin-related differences were not significant for SAMs in their early plastochron phase, to become obvious in a more advanced plastochron stage. Individual cell and nuclear measurements confirmed the rationale of distinguishing within SAM zones, characterized by specific cell and nuclear sizes liable to vary according to physiological age. The various histocytological investigations carried out established that SAM cell characteristics appeared to be the more reliable indicators of phase change. This was particularly true for the nucleoplasmic ratio and for more qualitative differences observed also at the nuclear level. SAM nuclei of the two in vitro origins were more evenly stained by naphtol blue-black, uniformly light for the juvenile source, whereas the mature source showed also darker nuclei. In contrast, SAM nuclei from outdoor origins had more chromocenters, darker and diffusely spread for the mature source than for the rejuvenated and the juvenile origins, where they were more peripherally distributed and where the nucleoli appeared more clearly. These results were discussed with respect to physiological ageing and in vitro culture influence, and suggest a determining influence of SAM cell nuclei on phase change phenomenon of arborescent species.

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Histocytological analysis of yam (Dioscorea alata) shoot tips cryopreserved by encapsulation–dehydration.

et al. 2013

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In this work, we performed qualitative and quantitative observations of the cytological changes occurring in cells of yam (Dioscorea alata) in vitro shoot tips cryopreserved using the encapsulation-dehydration (E-D) technique. Shoot tip osmoprotection for 24 h in 1.25 M sucrose medium induced drastic changes in cellular cytological features, including high plasmolysis in all three cellular areas studied, the external cell layer (L1), one to three (L1-3) and seven to nine (L7-9) cell layers from the surface of the meristematic dome, pyknotic nuclei in meristematic area cells and disappearance of nucleoli. Nucleus size decreased significantly in all cellular areas studied. Nucleocytoplasmic ratio decreased significantly in L1-3 and L7-9 cells. Nuclear protein content increased, particularly in L1 and L1-3 cells. After physical dehydration, plasma membrane of numerous basal part cells was broken and intracellular soluble protein leakage was observed. Nucleus area and nucleocytoplasmic ratio decreased significantly in L7-9 cells. One week after cryopreservation, shoot tips showed regrowth and living cells had recovered their original morphology. In all cellular areas studied, nuclei had retrieved their original staining and nucleoli were visible. Original nucleus area values were recovered in L1-3 and L1 cells. The nucleocytoplasmic ratio retrieved its initial value in L1 cells but remained at levels observed after osmoprotection for L1-3 and L7-9 cells. The nuclear protein content had retrieved its original level. This investigation provided new insights in changes occurring in D. alata apices throughout an E-D protocol.

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Phase change-related variations of dome shape in Eucalyptus urophylla × Eucalyptus grandis shoot apical meristems

Abstract: Shoot apical meristem (SAM) domes derived from five different outdoor and in vitro sources of juvenile and mature Eucalyptus urophylla 9 Eucalyptus grandis akin genotypes were compared.

Cited by 9 publications

References 34 publications

Characteristics of Acacia mangium shoot apical meristems in natural and in vitro conditions in relation to heteroblasty

Characteristics of Acacia mangium shoot apical meristems in natural and in vitro conditions in relation to heteroblasty

Histocytological characteristics of Eucalyptus urophylla × Eucalyptus grandis shoot apical meristems of different physiological ages

Histocytological analysis of yam (Dioscorea alata) shoot tips cryopreserved by encapsulation–dehydration.

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