Phase and Surface Properties of Lipid Bilayers Containing Neoglycolipids

Luzardo, Marı́a del Carmen; Bernik, Delia L.; Pazos, Fabiola; Figueroa, Santiago; Lanio, María E.; Vérez, Vicente; Disalvo, E.A.

doi:10.1006/abbi.1998.1017

Cited by 8 publications

(1 citation statement)

References 19 publications

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“…Then, we postulate that some components present in grape seed extract would promote a dehydration effect in the phospholipid water interface. A decrease of the water molecules movement due to a packing effect at the polar head groups should be discarded, since no effect was observed in the behavior of the TMA-DPH anisotropy probe, as observed for bilayers containing neoglycolipids (61).…”

Section: Discussionmentioning

confidence: 99%

Incorporation and Interaction of Grape Seed Extract in Membranes and Relation with Efficacy in Muscle Foods

Maestre

Micol

Funes

et al. 2010

J. Agric. Food Chem.

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The interaction and location of phenolic antioxidants in model membranes has been related with their effectiveness for inhibiting lipid oxidation of fish mince with the aim to identify mechanisms involved in the antioxidant effectiveness in muscle foods. For such scope, the effect of grape seed extract and its main components, catechin, epicatechin and procyanidin B(2) to be located and induce changes in phospholipid model membranes was studied by different biophysical techniques and related to their antioxidant efficiency. Grape seed extract showed the highest inhibition of oxidation in chilled minced fish muscle. Antioxidant in- vitro capacities were also studied but they did not show a clear relationship with the antioxidant efficiency found in fish muscle. The phospholipid/water partition coefficients and fluorescence quenching studies showed that procyanidin B(2) was located in a more internal location than monomeric catechin and epicatechin within the phospholipid palisade. Grape seed extract showed strongest effect compared to its main components in the increase of the lipid order at the DMPC fluid phase by fluorescence anisotropy measurements. Grape seed extract also promoted a dehydration effect in DMPC membranes at the phospholipid/water interface and resistance to solubilization by nonionic detergents in DMPC membranes. The presence of molecular linkages, probably by hydrogen bonding, is proposed between procyanidins (or some galloylated catechins) and the polar head groups of the phospholipids to account for the dehydration effect at the phospholipid/water interface and membrane-stabilizing effects. These effects may be directly related to the higher efficacy of grape seed extract to inhibit lipid oxidation in fish muscle, probably by hindering radical propagation.

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Section: Discussionmentioning

confidence: 99%

Incorporation and Interaction of Grape Seed Extract in Membranes and Relation with Efficacy in Muscle Foods

Maestre

Micol

Funes

et al. 2010

J. Agric. Food Chem.

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Lipid–Protein Interactions in Rat Renal Subcellular Membranes: A Biophysical and Biochemical Study

D’Antuono

Fernández-Tomé

Sterín-Speziale

et al. 2000

Archives of Biochemistry and Biophysics

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A Fluorescence Study on Binding Interaction of N-acetylated Dansylamide Conjugates with β-cyclodextrin, Tween-20 and DPPC Lipid Bilayer Membrane

Tripathi

2017

J Fluoresc

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The present work describes the photophysical behavior of a saturated fatty acid (palmitic acid) containing N-acetylated dansylamide derivative (DAN-PA) into biologically important organized assembly such as β-cyclodextrin (β-CD), Tween-20 (T-20) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) lipid bilayer membrane. The results were compared by using another N-acetylated dansylamide conjugate having a short hydrophobic tail, DAN-ACYL. Long hydrophobic tail (saturated fatty acid) containing dansylamide conjugate (DAN-PA) shows more efficient binding interactions with the β-CD as compared to the short tail containing dansylamide derivative (DAN-ACYL). The calculated binding constants values of DAN-PA and DAN-ACYL probes are 1.35 × 10 M and 0.31 × 10 M respectively. The DAP-PA is a sensitive fluorophore for understanding the micellization process in T-20, as compared to the DAN-ACYL because it shows a significant change in fluorescent properties (steady-state and time-resolved both) with changing in T-20 concentrations. The calculated CMC value for T-20 surfactant is 0.07 mM. While the DAN-ACYL does not show any change in the fluorescent properties while changing the T-20 concentrations. Fluorescent parameters like steady-state and time-resolved of DAN-PA are quite sensitive towards the thermo-tropic phase transitional changes into lipid bilayer membrane properties. And the calculated thermo-tropic phase transition temperature by using DAN-PA fluorophore is 42 °C.

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Phase and Surface Properties of Lipid Bilayers Containing Neoglycolipids

Cited by 8 publications

References 19 publications

Incorporation and Interaction of Grape Seed Extract in Membranes and Relation with Efficacy in Muscle Foods

Incorporation and Interaction of Grape Seed Extract in Membranes and Relation with Efficacy in Muscle Foods

Lipid–Protein Interactions in Rat Renal Subcellular Membranes: A Biophysical and Biochemical Study

A Fluorescence Study on Binding Interaction of N-acetylated Dansylamide Conjugates with β-cyclodextrin, Tween-20 and DPPC Lipid Bilayer Membrane

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