“…The spine analog is a 3D-printed CAD model of the human L3-L5 vertebrae, as described in chapter 3, in a gelatin/graphite speckle-producing background (72). The porcine tissue layer has two functions -to provide an optical target with surface texture broadly similar to human skin, and also to mimic the speed-of-sound variations and other phenomena that cause ultrasound phase aberration in human tissue (96), (97).…”