Pharyngeal Selective Brain Cooling Is Associated with Reduced CNS Cortical Lesion after Experimental Traumatic Brain Injury in Rats

Doll, Hinnerk; Maegele, Marc; Böhl, Jürgen; Störkel, Stephan; Kipfmueller, Florian; Schaefer, Ute; Angelov, Doychin N.; Wirth, Stéfan; Truebel, Hubert

doi:10.1089/neu.2010.1505

Cited by 6 publications

(4 citation statements)

References 48 publications

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“…Similar to our previous studies and other reports, TBI produces behavioral abnormalities and neurodegenerative changes resulting from regional patterns of cell death and circuit dysfunction [21,44]. As supported by previous studies of TTM on TBI, we also demonstrated that using CCI rats as an in vivo model that TTM attenuated several phenotypes of animal post-TBI, markedly reduced cell death [7,8,11], BBB leakage [45][46][47], cortical lesion volume [48], cerebrovascular histopathology [49,50], and improved behavioral and pathological outcomes [51]. These results may help determine both the safety and efficacy for postinjury TTM, and such laboratory models (in vitro or in vivo) may present an ideal opportunity for the application of proteomic technological approach to biomarker discovery after TBI.…”

Section: Discussionsupporting

confidence: 92%

iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management

Cheng

et al. 2018

Neurotherapeutics

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This study aimed to investigate the effects of targeted temperature management (TTM) modulation on traumatic brain injury (TBI) and the involved mechanisms using quantitative proteomics technology. SH-SY5Y and HT-22 cells were subjected to moderate stretch injury using the cell injury controller (CIC), followed by incubation at TTM (mild hypothermia, 32°C), or normothermia (37°C). The real-time morphological changes, cell cycle phase distribution, death, and cell viability were evaluated. Moderate TBI was produced by the controlled cortical impactor (CCI), and the effects of TTM on the neurological damage, neurodegeneration, cerebrovascular histopathology, and behavioral outcome were determined in vivo. Results showed that TTM treatment prevented TBI-induced neuronal necrosis in the brain, achieved a substantial reduction in neuronal death both in vitro and in vivo, reduced cortical lesion volume and neuronal loss, attenuated cerebrovascular histopathological damage, brain edema, and improved behavioral outcome. Using an iTRAQ proteomics approach, proteins that were significantly associated with TTM in experimental TBI were identified. Importantly, changes in four candidate molecules (plasminogen [PLG], antithrombin III [AT III], fibrinogen gamma chain [FGG], transthyretin [TTR]) were verified using TBI rat brain tissues and TBI human cerebrospinal fluid (CSF) samples. This study is one of the first to investigate the neuroprotective effects of TTM on the proteome of human and experimental models of TBI, providing an overall landscape of the TBI brain proteome and a scientific foundation for further assessment of candidate molecules associated with TTM for the promotion of reparative strategies post-TBI.

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Section: Discussionsupporting

confidence: 92%

iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management

Cheng

et al. 2018

Neurotherapeutics

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“…Doll et al showed that a selective brain cooling method called “pharyngeal selective brain cooling” was associated with improved neurofunctional, sensorimotor, and neurocognitive recovery following TBI in rats ( 17 ). These positive findings with behavioral factors were substantiated by brain histo-morphological outcomes that included preservation of cortical tissue ipsilateral to the lesion and superior physical recovery with reduced weight loss in the acute phase post-injury and a more robust weight gain thereafter ( 18 ).…”

Section: Discussionmentioning

confidence: 96%

An Experimental Study: Does the Neuroprotective Effect Increase When Hypothermia Deepens After Traumatic Brain Injury?

Girişgin¹,

Kalkan²,

Ergin³

et al. 2015

Iran Red Crescent Med J

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Background:Experimental approaches have been promising with the use of therapeutic hypothermia after Traumatic Brain Injury (TBI) whereas clinical data have not supported its efficacy.Objectives:This study aimed to investigate whether using selective deeper brain cooling correlates with a more neuroprotective effect on Intracranial Pressure (ICP) increments following TBI in rats.Materials and Methods:Adult male Sprague-Dawley rats (mean weight = 300 g; n = 25) were subjected to brain injury using a modified Marmarou method. Immediately after the onset of TBI, rats were randomized into three groups. Selective brain cooling was applied around the head using ice packages. Intracranial Temperature (ICT) and ICP were continuously measured at 0, 30, 60, 120, and 180 minutes and recorded for all groups. Group 1 (n = 5) was normothermia and was assigned as the control group. Group 2 (n = 10) received moderate hypothermia with a target ICT of between 32°C - 33°C and Group 3 (n = 10) was given a deeper hypothermia with a target ICT of below 32°C.Results:All subjects reached the target ICT by the 30th minute of hypothermia induction. The ICT was significantly different in Group 2 compared to Group 1 only at the 120th minute (P = 0.017), while ICP was significantly lower starting from the 30th minute (P = 0.015). The ICT was significantly lower in Group 3 compared to Groups 1 and 2 starting from the 30th minute (P = 0.001 and P = 0.003, respectively). The ICP was significantly lower in Group 3 compared to Group 1 starting from 30th minute (P = 0.001); however, a significant difference in ICP between Group 3 and Group 2 was observed only at the 180th minute (P = 0.047).Conclusions:Results of this study indicate that selective brain cooling is an effective method of decreasing ICP in rats; however, the deeper hypothermia caused a greater decrease in ICP three hours after hypothermia induction.

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“…There are previously multiple papers that suggested targeted brain cooling as a reasonable treatment option to patient with severe traumatic brain injury [21][22][23][24][25][26][27][28][29][30][31][32][33]. Targeted brain cooling is a good alternative to systemic hypothermia, as systemic hypothermia has serious side effects such as circulatory constrain, increased risk of infection, electrolyte imbalance, and coagulopathy [15][16][17][18].…”

Section: Current Evidences On the Usage Of Direct Brain Cooling In Trmentioning

confidence: 99%

Direct Brain Cooling in Treating Severe Traumatic Head Injury

Idris¹,

Yee²,

Kandasamy³

et al. 2019

Traumatic Brain Injury - Neurobiology, Diagnosis and Treatment

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There are scientific evidences that hypothermia provides a strong neuroprotective effect on the brain following traumatic insults. In this chapter, we describe the pathophysiology of severe head injury with emphasis on benefits of hypothermia. To support these hypothetical or theoretical benefits, we describe our previous study with very encouraging findings done on severe head injuries, treated with direct focal brain cooling, and monitored with intracranial pressure, cerebral perfusion pressure, brain oxygenation, and brain temperature. This chapter ends with our current and still ongoing study in which one of its main objectives is to innovate a direct focal brain cooling machine. This chapter briefly explains the technical part of this cooling machine.

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Pharyngeal Selective Brain Cooling Is Associated with Reduced CNS Cortical Lesion after Experimental Traumatic Brain Injury in Rats

Cited by 6 publications

References 48 publications

iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management

iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management

An Experimental Study: Does the Neuroprotective Effect Increase When Hypothermia Deepens After Traumatic Brain Injury?

Direct Brain Cooling in Treating Severe Traumatic Head Injury

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