Pharmacology of octopamine‐, dopamine‐, and 5‐hydroxytryptamine‐stimulated cyclic amp accumulation in the corpus cardiacum of the american cockroach, Periplaneta americana L

Abstract

A variety of potential receptor agonists were incubated with isolated corpora cardiaca from the American cockroach Periplaneta americana to determine their effects o n cyclic AMP production in this gland. Octopamine, dopamine, and 5-hydroxytryptamine elevated cyclic AMP levels in a dose-dependent manner with estimated K, values of 15.8, 1.7, and 1.1 pM, respectively, and their stimulation was found to be additive. Several vertebrate receptor antagonists were tested against the three amines and a preliminary pharmacological profile developed.

Malpighian tubules of larvalAedes aegypti are hormonally stimulated by 5-hydroxytryptamine in response to increased salinity

“…This is comparable to the affinity of the receptor from Periplaneta americana corpora cardiaca (14.1 µM) (Gole et al, 1987). However, (+)-butaclamol was the only compound used in the present study which was also tested by Gole et al (1987). Similarly, insufficient data is available to compare the Gryllus mandibular closer muscle 5-HT receptor (Baines and Downer, 1991), the Schistocerca foregut receptor (Banner et al, 1987), the Drosophila receptor described by Witz et al (1990), or the Locusta brain 5-HT receptor described by Hiripi and Downer (1993).…”

“…The larval Aedes aegypti Malpighian tubule has much lower affinity for (+)-butaclamol, with an apparent K b of only 19 µM. This is comparable to the affinity of the receptor from Periplaneta americana corpora cardiaca (14.1 µM) (Gole et al, 1987). However, (+)-butaclamol was the only compound used in the present study which was also tested by Gole et al (1987).…”

Modulation of motor behavior by dopamine and the D1-like dopamine receptor AmDOP2 in the honey bee

“…Flupenthixol has been reported to act as a weak antagonist at octopamine receptors in the cockroach (Gole et al, 1987). However, flupenthixol injection has a distinct (and much more profound) effect on the behavioral profile of honey bees than injection of the octopamine and tyramine receptor antagonists mianserin and yohimbine did in an experimental protocol identical to the one presented here (Fussnecker et al, 2006).…”

Pharmacological characterization of a 5‐hydroxytryptamine‐sensitive receptor/adenylate cyclase complex in the mandibular closer muscles of the cricket, Gryllus domestica

“…In the cockroach brain, e.g., gramine is a much better antagonist of 5-HT than either cyproheptadine or mianserin [ll]. However, for peripherally located receptors such as the cricket muscle receptor, blowfly salivary gland receptor, and the cockroach corpora cardiacum receptor, cyproheptadine and mianserin are better antagonists than gramine [10,19], This situation is comparable to that reported in the vertebrate, where pharmacology of the centrally located 5-HT-adenylate cyclase receptor differs from those located peripherally [20].…”

“…The systems that have been studied in any detail include the blowfly salivary glands [7], locust foregut [8,9], and cockroach nervous system [10,13]. Thus, the present study on the pharmacology of the 5-HT response of the mandibular muscles extends this catalogue to include skeletal muscle.…”