Pharmacology mechanism of <i>Flos magnoliae</i> and <i>Centipeda minima</i> for treating allergic rhinitis based on pharmacology network

Liang, Yulin; Zhang, Xiaofei; Zou, Junbo; Shi, Yajun; Wang, Yu; Tai, Jia; Yang, Yanjun; Zhou, Xiao; Guo, Deliang; Wang, Jing; Cheng, Jiangxue; Yang, Ming

doi:10.1080/03639045.2019.1635150

Cited by 23 publications

(19 citation statements)

References 16 publications

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“…(B) The molecular formulas of the 11 bioactive components identified from Huachansu capsules (HCSCs). and 2, the overlapping genes for compound targets and HCCassociated human genes was obtained by using Venny 2.1.0 (Liang et al, 2019). As a result, 82 HCC-related genes were identified for the 11 components of HCSCs ( Table 2).…”

Section: Hcc-related Target Identification Of Hcscsmentioning

confidence: 99%

Interpreting the Pharmacological Mechanisms of Huachansu Capsules on Hepatocellular Carcinoma Through Combining Network Pharmacology and Experimental Evaluation

et al. 2020

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Hepatocellular carcinoma (HCC) is one of the most fatal cancers across the world. Chinese medicine has been used as adjunctive or complementary therapy for the management of HCC. Huachansu belongs to a class of toxic steroids isolated from toad venom that has important anti-cancer property. This study was aimed to identify the bioactive constituents and molecular targets of Huachansu capsules (HCSCs) for treating HCC using network pharmacology analysis and experimental assays. The major bioactive components of HCSCs were determined using ultra-performance liquid chromatographytandem mass spectrometry (UPLC-MS/MS). A series of network pharmacology methods including target prediction, pathway identification, and network establishment were applied to identify the modes of action of HCSCs against HCC. Furthermore, a series of experiments, including MTT, clonogenic assay, 3-D transwell, wound healing assay, as well as flow cytometry, were conducted to verify the inhibitory ability of HCSCs on HCC in vitro. The results showed that 11 chemical components were identified from HCSCs. The network pharmacological analysis showed that there were 82 related anti-HCC targets and 14 potential pathways for these 11 components. Moreover, experimental assays confirmed the inhibitory effects of HCSCs against HCC in vitro. Taken together, our study revealed the synergistic effects of HCSCs on a systematic level, and suggested that HCSCs exhibited anti-HCC effects in a multi-component, multi-target, and multipathway manner.

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Section: Hcc-related Target Identification Of Hcscsmentioning

confidence: 99%

Interpreting the Pharmacological Mechanisms of Huachansu Capsules on Hepatocellular Carcinoma Through Combining Network Pharmacology and Experimental Evaluation

et al. 2020

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“…Based on the above, the previous compound targets are mapped with the genes of human CCVD-correlative disease [36]. Afterwards, the GO interactive network and the bubble diagram of KEGG pathways were structured based on the topGO packet of R platform [37]. P value was calculated in these two enrichment analyses, and P < 0.05 suggested the enrichment degree of statistically significant [38].…”

Section: Target Fishing Drug Target Indentifying Is Momentousmentioning

confidence: 99%

“…We extracted the precise pathway information of targets from the database of KEGG, and then constructed a target-pathway bipartite graph that comprises targets and their corresponding normative pathways. e previous two were generated in Cytoscape 3.7.2 software that is a standard tool for biological network visualization and data integration to further analyze the mutual relations in the network [37,39]. (3) Protein-protein interaction (PPI network) was mapped using important pathway-related targets for screening core target proteins based on (STRING) database (https://string-db.org/).…”

Section: Network and Protein-protein Interaction Datamentioning

confidence: 99%

A Systems Pharmacology Approach for Identifying the Multiple Mechanisms of Action for the Rougui‐Fuzi Herb Pair in the Treatment of Cardiocerebral Vascular Diseases

Liu

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Cardiocerebral vascular diseases (CCVDs) are the main reasons for high morbidity and mortality all over the world, including atherosclerosis, hypertension, myocardial infarction, stroke, and so on. Chinese herbs pair of the Cinnamomum cassia Presl (Chinese name, rougui) and the Aconitum carmichaelii Debx (Chinese name, fuzi) can be effective in CCVDs, which is recorded in the ancient classic book Shennong Bencao Jing, Mingyibielu and Thousand Golden Prescriptions. However, the active ingredients and the molecular mechanisms of rougui-fuzi in treatment of CCVDs are still unclear. This study was designed to apply a system pharmacology approach to reveal the molecular mechanisms of the rougui-fuzi anti-CCVDs. The 163 candidate compounds were retrieved from Traditional Chinese Medicine System Pharmacology Database and Analysis Platform (TCMSP). And 84 potential active compounds and the corresponding 42 targets were obtained from systematic model. The underlying mechanisms of the therapeutic effect for rougui-fuzi were investigated with gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Then, component-target-disease (C-T-D) and target-pathway (T-P) networks were constructed to further dissect the core pathways, potential targets, and active compounds in treatment of CCVDs for rougui-fuzi. We also constituted protein-protein in interaction (PPI) network by the reflect target protein of the crucial pathways against CCVDs. As a result, 21 key compounds, 8 key targets, and 3 key pathways were obtained for rougui-fuzi. Afterwards, molecular docking was performed to validate the reliability of the interactions between some compounds and their corresponding targets. Finally, UPLC-Q-Exactive-MSE and GC-MS/MS were analyzed to detect the active ingredients of rougui-fuzi. Our results may provide a new approach to clarify the molecular mechanisms of Chinese herb pair in treatment with CCVDs at a systematic level.

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“…Pharmacological experiments have indicated that the volatile oils of drugs used in Traditional chinese Medicine (TCM) have anti-inflammatory and anti-allergenic effects, and they are currently used to treat acute and chronic rhinitis, as well as ar. in particular, the volatile oils of FM and cM serve important roles in the treatment of ar (10,11). due to the low and unstable extraction rate of volatile oil, constituents such as eucalyptol and geraniene d were easy to volatilize (12).…”

Section: Introductionmentioning

confidence: 99%

“…The association between compatibility and pharmacodynamics of FM and cM has been rarely studied. our previous study revealed that the combination of volatile oil from FM and CM may effectively relieve local inflammatory cell infiltration and cell necrosis of nasal mucosa in the nasal cavity of rats, thereby decreasing the histamine content in blood and improving the symptoms of ar (11). in Bitongning drops, the active component is aromatic water.…”

Section: Introductionmentioning

confidence: 99%

Preparation and quality evaluation of a volatile oil microemulsion from Flos magnoliae and Centipeda minima

et al. 2020

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in order to improve the water solubility of the volatile oils extracted from Flos magnoliae (FM) and Centipeda minima (cM), they were prepared as a microemulsion (Me), which were then used in the development of an FM and cM volatile oil Me for the treatment of allergic rhinitis (ar). Me was prepared by phase inversion emulsification, and the prescription factors such as emulsifier, co-emulsifier, oil phase, Km, which represents the ratio of the mass of emulsifier to that of the co-emulsifier, and preparation factors such as temperature affecting the formation of the Me were selected according to the formation area of Me in a pseudo-ternary phase diagram. The quality of the Me was evaluated based on its appearance, particle size, Zeta potential and stability. The content of eucalyptol in Me was determined by gas chromatography-mass spectrometry (Gc-MS). The cumulative permeability of the Me within 24 h was measured with a transdermal diffusion tester. The results revealed that the best formula for preparation of the Me was as follows: Castor oil polyoxyethylene ether (EL-40) was the emulsifier; the co-emulsifier was anhydrous ethanol; the Km was 2:1; the mixed phase of volatile oil and isopropyl myristate with mass ratio of 1:1 was used as oil phase; and the preparation temperature was 25˚C. The content of eucalyptol in the ME was 2.57 mg/g, and the cumulative permeability of the Me in 24 h was significantly increased compared with that of the reference oil solution. The appearance of the Me was uniform, and the solution was transparent. in conclusion, compared with traditional preparations, FM and cM volatile oil Me is a novel, improved and more effective preparation for the treatment of ar.

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Pharmacology mechanism of Flos magnoliae and Centipeda minima for treating allergic rhinitis based on pharmacology network

Cited by 23 publications

References 16 publications

Interpreting the Pharmacological Mechanisms of Huachansu Capsules on Hepatocellular Carcinoma Through Combining Network Pharmacology and Experimental Evaluation

Interpreting the Pharmacological Mechanisms of Huachansu Capsules on Hepatocellular Carcinoma Through Combining Network Pharmacology and Experimental Evaluation

A Systems Pharmacology Approach for Identifying the Multiple Mechanisms of Action for the Rougui‐Fuzi Herb Pair in the Treatment of Cardiocerebral Vascular Diseases

Preparation and quality evaluation of a volatile oil microemulsion from Flos magnoliae and Centipeda minima

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