Pharmacological Topics of Bone Metabolism: A Novel Bisphosphonate for the Treatment of Periodontitis

Satoh, Hisashi; Takeyama, Sadaaki; Suzuki, Keiko; Murakami, Shohei; Yamada, Shoji

doi:10.1254/jphs.fm0070272

Cited by 29 publications

(25 citation statements)

References 14 publications

(4 reference statements)

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“…Moreover, it can impede the conversion of dicalcium phosphate dehydrate (DCPD) to hydroxyapatite and reduce pellicle formation. Bisphosphonates, which are stable synthetic analogs of pyrophosphate with resistance to hydrolysis, have also been used as anti-calculus agents (Shinoda et al, 2008;Sikder et al, 2004). When used as advanced mineralization inhibitors, polyphosphates (e.g., hexametaphosphate) can reduce calculus formation (Sikder et al, 2004).…”

Section: Strategies For Prevention Of Calculus Formationmentioning

confidence: 99%

Detection, removal and prevention of calculus: Literature Review

Kamath

Nayak

2014

The Saudi Dental Journal

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Dental plaque is considered to be a major etiological factor in the development of periodontal disease. Accordingly, the elimination of supra- and sub-gingival plaque and calculus is the cornerstone of periodontal therapy. Dental calculus is mineralized plaque; because it is porous, it can absorb various toxic products that can damage the periodontal tissues. Hence, calculus should be accurately detected and thoroughly removed for adequate periodontal therapy. Many techniques have been used to identify and remove calculus deposits present on the root surface. The purpose of this review was to compile the various methods and their advantages for the detection and removal of calculus.

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Section: Strategies For Prevention Of Calculus Formationmentioning

confidence: 99%

Detection, removal and prevention of calculus: Literature Review

Kamath

Nayak

2014

The Saudi Dental Journal

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“…Bisphosphonates (BPs) are pyrophosphate analogs that can suppress osteoclastic bone resorption[46] Bisphosphonates are known to bind to hydroxyapatite crystals and prevent their dissolution in addition to increasing osteoblast differentiation and inhibiting osteoclast activation. [4] Giannobile WV has summarized and categorized the bone-specific actions of bisphosphonates at the tissue level (decrease bone turnover due to inhibition of bone resorption, decrease the number of new bone multicellular units resulting in a net positive whole body bone balance) and at cellular level (decrease osteoclast recruitment, osteoclast adhesion, depth of resorption site and release of cytokines by macrophages along with an increase in osteoclast apoptosis and osteoblast differentiation and number).…”

Section: Host Modulatory Therapymentioning

confidence: 99%

Host modulation therapy: An indispensable part of perioceutics

Gulati

Anand

Govila

et al. 2014

J Indian Soc Periodontol

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Traditionally, only antimicrobials have been used as the chemotherapeutic modality for the treatment of periodontitis. Though bacteria are the primary etiologic factors of periodontal diseases, yet the extent and severity of tissue destruction seen in periodontitis is determined by the host immuno-inflammatory response to these bacteria. This increasing awareness and knowledge of the host-microbial interaction in periodontal pathogenesis has presented the opportunity for exploring new therapeutic strategies for periodontitis by means of targeting host response via host-modulating agents. This has lead to the emergence of the field of “Perioceutics” i.e. the use of parmacotherapeutic agents including antimicrobial therapy as well as host modulatory therapy for the management of periodontitis. These host-modulating agents used as an adjunct tip the balance between periodontal health and disease progression in the direction of a healing response. In this article the host-modulating role of various systemically and locally delivered perioceutic agents will be reviewed.

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“…TRK-530 is a recently developed novel synthetic bisphosphonate with an anti-oxidant methylthio-phenylthio side chain (5). The nitrogen-containing bisphosphonates used were alendronate (Sigma-Aldrich, St. Louis, MO, USA), risedronate (Procter and Gamble Pharmaceutics, Wood Corners Laboratories, Norwich, NY, USA) and zoledronate (Novartis Pharma AG, Switzerland).…”

Section: Reagents (Bisphosphonates)mentioning

confidence: 99%

“…1). It has been reported that the pharmacological characteristics of bisphosphonates vary depending on the nature of the side chains (5). Bisphosphonates are also classified into nitrogen-containing and non-nitrogen-containing bisphosphonates.…”

Section: Introductionmentioning

confidence: 99%

Effects of bisphosphonates on osteoclastogenesis in RAW264.7 cells

Yoshimura

2012

Int J Mol Med

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Abstract.Bisphosphonates are used as therapeutic agents for the management of osteoporosis and other bone diseases. However, the precise effects and mechanisms of bisphosphonates on osteoclastogenesis are unclear, as previous studies have reported contradictory findings and no studies have circumstantially assessed the effects of bisphosphonates on osteoclastogenesis. Therefore, the aim of this study was to determine the effects of bisphosphonates on osteoclastogenesis in RAW264.7 (RAW) cells. To examine the direct effects of bisphosphonates on osteoclast differentiation via receptor activator of nuclear factor-κB (RANK) ligand (RANKL), RAW cells were cultured with bisphosphonates. Addition of bisphosphonates to RAW cells led to a significant decrease in the number of osteoclasts and large osteoclasts (≥8 nuclei) in a bisphosphonate concentration-dependent and time-dependent manner. The cytotoxicity of non-nitrogen-containing bisphosphonates was specific to osteoclasts, while nitrogen-containing bisphosphonates were cytotoxic and induced cell death in both osteoclasts and RAW cells. Resorption activity was significantly diminished by treatment with bisphosphonates, thus confirming that bisphosphonates impair the absorptive activity of osteoclasts. We also investigated the effects of bisphosphonates on the mRNA expression of genes associated with osteoclastogenesis, osteoclast-specific markers and apoptosis-related genes using quantitative real-time PCR. The results suggest that bisphosphonates suppress osteoclast differentiation and infusion, and induce osteoclast apoptosis. With regard to osteoclast apoptosis induced by bisphosphonates, we further investigated the detection of DNA fragmentation and Caspase-Glo 3/7 assay. DNA fragmentation was confirmed after treatment with bisphosphonates, while caspase-3/7 activity increased significantly when compared with controls. In conclusion, bisphosphonates directly inhibited RANKLstimulated osteoclast differentiation and fusion in RAW cells. It was confirmed that bisphosphonates impair osteoclast resorption activity and induce apoptosis. The effects of nonnitrogen-containing bisphosphonates were also specific to osteoclasts, while nitrogen-containing bisphosphonates were cytotoxic and induced cell death in both osteoclasts and RAW cells.

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Pharmacological Topics of Bone Metabolism: A Novel Bisphosphonate for the Treatment of Periodontitis

Cited by 29 publications

References 14 publications

Detection, removal and prevention of calculus: Literature Review

Detection, removal and prevention of calculus: Literature Review

Host modulation therapy: An indispensable part of perioceutics

Effects of bisphosphonates on osteoclastogenesis in RAW264.7 cells

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