“…The cytokine cocktail included 0.1 ng/μL SCF (StemCell Technologies, catalog 02630), 0.05 ng/μL IL-3 (StemCell Technologies, catalog 02503), 0.04 ng/μL IL-6 (Miltenyi Biotech, catalog 130-095-365), 0.2 ng/μL GM-CSF (Peprotech, catalog 300–03), 0.2 ng/μL G-CSF (Peprotech, catalog 300–23), 0.004 U/mL Erithropoietin (StemCell, catalog 02625), 0.94 μg/μL Transferrin (Sigma Aldrich, catalog T8158), 0.1 ng/μL 2-Mercaptoethanol (Sigma Aldrich, catalog M7154). To identify live leukemic cells by flow cytometry, two antibodies that unequivocally identify the pathologic cell population in the patient samples were selected in combination with annexin V. Those cells without annexin V staining and with appropriate markers were considered live leukemic cells, as described previously [54, 55]. Proliferation inhibition was measured as the difference in the number of live leukemic cells in a well with drugs versus the vehicle control treated wells.…”