Pharmacological modulation of the antioxidant enzymes activities and the concentration of peroxidation products in fibroblasts stimulated with elastin peptides

Gmiński, Jan; Węglarz, Ludmiła; Drózdz, M; Goss, M

doi:10.1016/0306-3623(91)90012-u

Cited by 9 publications

(11 citation statements)

References 22 publications

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“…To date, it has been described that EDPs induce ROS production in murine monocyte and human fibroblasts [ 17 , 18 , 27 ]. Gmiński et al [ 19 ] stated that κ-elastin increased both the activity of antioxidant enzymes (SOD, CAT, GSH-Px) and the lipid peroxide concentration within fibroblasts [ 19 ]. On the other hand, EDPs decrease ROS production in human neutrophil cells, which suggests that EDP effects are cell-/tissue-dependent [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…Simultaneously, EDPs have been reported to induce ROS production in monocytes and human fibroblasts, thus accelerating the ROS generation process [ 17 , 18 ]. On the other hand, EDPs also increase the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) or glutathione peroxidase (GSH-Px), with a simultaneous increase in lipid peroxidation in human fibroblasts [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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The VGVAPG Peptide Regulates the Production of Nitric Oxide Synthases and Reactive Oxygen Species in Mouse Astrocyte Cells In Vitro

Szychowski

Gmiński

2019

Neurochem Res

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The products of elastin degradation, namely elastin-derived peptides (EDPs), are detectable in the cerebrospinal fluid of healthy individuals and in patients after ischemic stroke, and their number increases with age. Depending on their concentrations, both nitric oxide (NO) and reactive oxygen species (ROS) take part either in myocardial ischemia reperfusion injury or in neurovascular protection after ischemic stroke. The aim of our study was to determine the impact of VGVAPG peptide on ROS and NO production and expression of endothelial nitric oxide synthase (eNos), inducible nitric oxide synthase (iNos) and neuronal nitric oxide synthase (nNos) in mouse cortical astrocytes in vitro. Primary astrocytes were maintained in DMEM/F12 without phenol red supplemented with 10% fetal bovine serum. The cells were exposed to rising VGVAPG peptide concentrations, and ROS and NO production was measured. After 6 h (for mRNA) and 24 (for the protein) of exposure to 10 nM and 1 µM of the peptide, expression of nNos, iNos and eNos was measured. Moreover, the Glb1 siRNA gene knockdown method and Pioglitazone, a peroxisome proliferator-activated receptor gamma (Pparγ) agonist, were applied. Our study shows that the VGVAPG peptide decreased eNos, iNos and nNos mRNA and protein expression in mouse astrocytes in vitro. The VGVAPG peptide also decreased NO production while increasing ROS production in the cells. Furthermore, silencing of the Glb1 gene reversed all effects caused by the VGVAPG peptide. However, due to the lack of sufficient data explaining the molecular mechanism of action of the VGVAPG peptide in the nervous system, more studies in this area are necessary.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The VGVAPG Peptide Regulates the Production of Nitric Oxide Synthases and Reactive Oxygen Species in Mouse Astrocyte Cells In Vitro

Szychowski

Gmiński

2019

Neurochem Res

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“…Our previous research showed that ROS levels increase during stimulation with VGVAPG peptide in mouse primary astrocytes in vitro (Szychowski and Gmiński 2019a). It has been described that EDPs enhance the activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), or glutathione peroxidase (GPx), and increase lipid peroxidation in human fibroblasts (Gmiński et al 1991). Moreover, the VGVAPG peptide reduced ROS production in neutrophils in control patients and those with stable chronic obstructive pulmonary disease (COPD) (Dupont et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative Effect of Elastin-Derived Peptide VGVAPG on SH-SY5Y Neuroblastoma Cells

et al. 2019

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Throughout the lifetime of humans, the amount of stem cells and the rate of cell proliferation continue to decrease. Reactive oxygen species (ROS) are one among the many factors that promote stem cell aging. Both a decrease in the level of stem cells and increase in ROS production can lead to the development of different neurodegenerative diseases. This study was conducted to determine how the VGVAPG peptide, liberated from elastin during the aging process and under pathological conditions, affects ROS production and activities of antioxidant enzymes in undifferentiated, proliferating SH-SY5Y cells. SH-SY5Y cells were maintained in Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 supplemented with 10% heat-inactivated fetal bovine serum (FBS). After treating the SH-SY5Y cells with VGVAPG peptide, we measured ROS production; cell metabolism, proliferation, and expression; and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT). We demonstrated that the VGVAPG peptide increases GPx expression and activity, whereas it decreases CAT expression in SH-SY5Y cells. Silencing of the GLB1 gene prevents changes in GPx activity. Despite the fact that the VGVAPG peptide increases GPx expression, it increases the ROS level. Moreover, the VGVAPG peptide decreases SH-SY5Y proliferation, which is prevented by the ROS scavenger N-acetyl-L-cysteine. Our data suggest that ROS production and decreased proliferation of SH-SY5Y cells are the results of excitotoxicity meditated through close unrecognized molecular pathways. More research is needed to elucidate the unknown mechanism of action of VGVAPG peptide in the nervous system.

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“…Gminski et al (22) attempted to determine whether the activity of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) in fibroblasts stimulated with kappa-elastin can be modulated by nifedipine. The addition of kappa-elastin to the fibroblasts caused an increase in the activity of the antioxidant enzymes, reflecting an increase in the concentration of biologically active oxygen species.…”

Section: Free Radical-induced Oxidationmentioning

confidence: 99%

“…Several studies mention that control experiments with the solvent used to dissolve the drugs were made such that the final solvent concentration in the oxidation reaction did not affect lipid oxidation (7,14,18,22,24,38,39). However, one can question the effect of the solvent on the interpretation of the results where there is no mention of solvent controls.…”

Section: Structure-function Relationshipmentioning

confidence: 99%

Calcium Channel Antagonists as Antioxidants

Rojstaczer¹,

Triggle²

1994

Cardiovascular Drug Reviews

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Pharmacological modulation of the antioxidant enzymes activities and the concentration of peroxidation products in fibroblasts stimulated with elastin peptides

Cited by 9 publications

References 22 publications

The VGVAPG Peptide Regulates the Production of Nitric Oxide Synthases and Reactive Oxygen Species in Mouse Astrocyte Cells In Vitro

The VGVAPG Peptide Regulates the Production of Nitric Oxide Synthases and Reactive Oxygen Species in Mouse Astrocyte Cells In Vitro

Antiproliferative Effect of Elastin-Derived Peptide VGVAPG on SH-SY5Y Neuroblastoma Cells

Calcium Channel Antagonists as Antioxidants

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