Pharmacological modulation of anxiety-like phenotypes in adult zebrafish behavioral models

Stewart, Adam; Wu, Nadine; Cachat, Jonathan; Hart, Peter C.; Gaikwad, Siddharth; Wong, Keith; Utterback, Eli; Gilder, Thomas; Kyzar, Evan J.; Newman, Alan; Carlos, Dillon; Chang, Katie; Hook, Molly; Rhymes, Catherine; Caffery, Michael; Greenberg, Mitchell; Zadina, James E.; Kalueff, Allan V.

doi:10.1016/j.pnpbp.2010.11.035

Cited by 199 publications

(130 citation statements)

References 194 publications

(233 reference statements)

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“…Activation of the ligand-gated GABA A receptor is the major inhibitory mechanism in the central nervous system of vertebrate animals, and is involved in reducing anxiety in humans [15], rodents [16] and fish [16,17]. GABA A receptors are the main target for pharmaceuticals that decrease anxiety (anxiolytics) such as benzodiazepines, which have been found in surface waters at concentrations that can alter food intake, social behaviour and activity levels in fish [18].…”

Section: Introductionmentioning

confidence: 99%

CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning

2014

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The average surface pH of the ocean is dropping at a rapid rate due to the dissolution of anthropogenic CO 2 , raising concerns for marine life. Additionally, some coastal areas periodically experience upwelling of CO 2 -enriched water with reduced pH. Previous research has demonstrated ocean acidification (OA)-induced changes in behavioural and sensory systems including olfaction, which is due to altered function of neural gamma-aminobutyric acid type A (GABA A ) receptors. Here, we used a camera-based tracking software system to examine whether OA-dependent changes in GABA A receptors affect anxiety in juvenile Californian rockfish (Sebastes diploproa). Anxiety was estimated using behavioural tests that measure light/dark preference (scototaxis) and proximity to an object. After one week in OA conditions projected for the next century in the California shore (1125 + 100 matm, pH 7.75), anxiety was significantly increased relative to controls (483 + 40 matm CO 2 , pH 8.1). The GABA A -receptor agonist muscimol, but not the antagonist gabazine, caused a significant increase in anxiety consistent with altered Cl 2 flux in OA-exposed fish. OA-exposed fish remained more anxious even after 7 days back in control seawater; however, they resumed their normal behaviour by day 12. These results show that OA could severely alter rockfish behaviour; however, this effect is reversible.

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Section: Introductionmentioning

confidence: 99%

CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning

2014

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“…Zebrafish larvae are ideally suited for high-throughput analyses because of their small size 7,8 . Software is commercially available for automated analyses of behavior in larval and adult zebrafish [9][10][11][12][13][14] . However, this software can be expensive and has limited options for measuring complex behaviors of zebrafish larvae in multi-well plates.…”

Section: Introductionmentioning

confidence: 99%

Automated High-throughput Behavioral Analyses in Zebrafish Larvae

Richendrfer

Créton

2013

JoVE

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We have created a novel high-throughput imaging system for the analysis of behavior in 7-day-old zebrafish larvae in multi-lane plates. This system measures spontaneous behaviors and the response to an aversive stimulus, which is shown to the larvae via a PowerPoint presentation. The recorded images are analyzed with an ImageJ macro, which automatically splits the color channels, subtracts the background, and applies a threshold to identify individual larvae placement in the lanes. We can then import the coordinates into an Excel sheet to quantify swim speed, preference for edge or side of the lane, resting behavior, thigmotaxis, distance between larvae, and avoidance behavior. Subtle changes in behavior are easily detected using our system, making it useful for behavioral analyses after exposure to environmental toxicants or pharmaceuticals.

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“…Ten post-treatment larvae/group were analyzed for stereotypic behavior (Stewart et al, 2013;Stewart et al, 2011) on 3 and 7 dpf and repeated thrice (total 30 larvae/group). Circle swimming was monitored on larvae, placed in 54mm diameter Petri dish filled with water at 27°C, following a touch of stimulus on their tail.…”

Section: Behavior Assaysmentioning

confidence: 99%

Monosodium Glutamate Exposure at Early Developmental Stage Increases Apoptosis and Stereotypic Behavior Risks on Zebrafish (Danio Rerio) Larvae

Kurnianingsih

2016

Indonesian J. Pharm.

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Excessive glutamate may give neurotoxic effects and contribute to Autism spectrum disorder(ASD). In this study, we investigated prolonged exposure effects of 10 µg/mL Monosodium Glutamate (MSG) on intracellular calcium level, bax, bcl-2, ratio of bax/bcl-2 genes expression, caspase-3, apoptosis of brain cells and stereotypic behavior of Zebrafish (Danio rerio) larvae at early developmental stages. Genes expression were determined by real time PCR, caspase-3 using ELISA, intracellular Ca 2+ and apoptotic cells of brain using confocal microscopy, locomotor activity by using crossing lines assay whereas stereotypic behavior by circle swimming. The results indicated that MSG exposure increased brain bax and bcl-2; and caspase-3; intracellular Ca 2+ ; and apoptosis; stereotypic behavior; and decreased locomotor activity. Termination of MSG treatments resulted in recovery of bax, bcl-2, caspase-3 basal levels and stereotypic behavior. In conclusion, MSG exposure at early embryonic stage increased brain cell damage and risk of behavior changes.

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Pharmacological modulation of anxiety-like phenotypes in adult zebrafish behavioral models

Cited by 199 publications

References 194 publications

CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning

CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning

Automated High-throughput Behavioral Analyses in Zebrafish Larvae

Monosodium Glutamate Exposure at Early Developmental Stage Increases Apoptosis and Stereotypic Behavior Risks on Zebrafish (Danio Rerio) Larvae

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Pharmacological modulation of anxiety-like phenotypes in adult zebrafish behavioral models

Cited by 199 publications

References 194 publications

CO2-induced ocean acidification increases anxiety in Rockfish via alteration of GABAAreceptor functioning

CO2-induced ocean acidification increases anxiety in Rockfish via alteration of GABAAreceptor functioning

Automated High-throughput Behavioral Analyses in Zebrafish Larvae

Monosodium Glutamate Exposure at Early Developmental Stage Increases Apoptosis and Stereotypic Behavior Risks on Zebrafish (Danio Rerio) Larvae

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CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning

CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning