Pharmacological investigation on the wound healing effects of Radix Rehmanniae in an animal model of diabetic foot ulcer

Lau, David Tai-Wai; Lam, F.; Lau, Kin Mang; Chan, Judy Yuet-Wa; Lee, K.M.; Sahota, Daljit Singh; Ho, Yu‐Ling; Fung, Kwok‐Pui; Leung, Ping Chung; Lau, Clara Bik San

doi:10.1016/j.jep.2009.02.010

Cited by 77 publications

(48 citation statements)

References 38 publications

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“…Resistant Bacterial Infected Wound in Animal Model This was achieved as described by Lau et al (2009) [31].…”

Section: Studying the Efficacy Of Tested Antibiotics Tenoxicam And Tmentioning

confidence: 99%

“…A rectangular pattern was marked on the dorsal surface of the foot using a flexible transparent plastic template, and then a layer of skin in full thickness with standard area of 2 mm × 5 mm was removed, Figure 1. The initial wound size was measured on day 1 [31].…”

Section: Studying the Efficacy Of Tested Antibiotics Tenoxicam And Tmentioning

confidence: 99%

“…Evaluation of wound healing: Observations of wound surface provided information on the gross extent of wound healing and improved wound healing by facilitating tissue regeneration [31]. In this study we selected the one-point sampling method because of the relatively small size of wounds that can be induced in rats and to better avoid contamination with peri-wound flora [18].…”

Section: Studying the Efficacy Of Tested Antibiotics Tenoxicam And Tmentioning

confidence: 99%

“…Carrageenan-induced paw inflammation rat model was used for inflammation study and evaluation of antiinflammatory activity [31] [37]. Carrageenan is sulfated polysaccharide derived from marine algae [38].…”

Section: Assessment Of Possible Mechanisms Of Tested Antibiotics Beyomentioning

confidence: 99%

See 3 more Smart Citations

Efficacy of Some Antibiotics in Curing Resistant <i>Escherichia coli</i> Infection

El-Banna¹,

El-Aziz²,

El-Mahdy³

et al. 2015

JBM

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There is growing interest in re-evaluation of older antibiotics with the wide spread of pathogen resistance, especially gram negative bacteria, which impair treatment of some infections. In contrast various studies have reported that some antibiotics have efficacy in clearing resistant bacterial infections. On account of that it was interesting to evaluate the efficacy of erythromycin, chloramphenicol and/or tenoxicam in curing and/or relieving wound infection of highly resistant Escherichia coli and investigate the possible mechanisms beyond their antibacterial activity. This was achieved through evaluating highly resistant E. coli strains in vitro using agar dilution and in vivo rat models of E. coli infected wound and acute inflammation by carrageenin, where possible mechanisms were evaluated through measuring immunological mediators and histopathological examination. This study revealed that in vivo, erythromycin alone or in combination with tenoxicam significantly improved the healing of infected skin wounds with E. coli irresspective of resistancy in vitro. In addition to the improvement of immunological mediators involved in inflammatory reaction, oxidative stress and in cytokines expression as response to the bacterial infection in vivo. On the other hand chloramphenicol neither alone nor in combination with tenoxicam, achieved any significant effect. Tenoxicam didn't show antimicrobial activity alone nor in combination with tested antibiotics in vitro, but it has shown synergestic activity in combination with tested antibiotics in vivo. Thus we concluded that immunomodulatory activity of erythromycin through anti-inflammatory and antioxidant effects was the possible mechanisms by which this antibiotic had healed infection with resistant E. coli in vivo, despite its resistancy to this antibiotic in vitro.

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“…Resistant Bacterial Infected Wound in Animal Model This was achieved as described by Lau et al (2009) [31].…”

Section: Studying the Efficacy Of Tested Antibiotics Tenoxicam And Tmentioning

confidence: 99%

Section: Studying the Efficacy Of Tested Antibiotics Tenoxicam And Tmentioning

confidence: 99%

Section: Studying the Efficacy Of Tested Antibiotics Tenoxicam And Tmentioning

confidence: 99%

Section: Assessment Of Possible Mechanisms Of Tested Antibiotics Beyomentioning

confidence: 99%

See 2 more Smart Citations

Efficacy of Some Antibiotics in Curing Resistant <i>Escherichia coli</i> Infection

El-Banna¹,

El-Aziz²,

El-Mahdy³

et al. 2015

JBM

View full text Add to dashboard Cite

show abstract

“…Chinese medicine is widely used for different diseases today. Previous studies show that Astragalus membranaceus Bunge (Leguminosae) (RA) and Rehmannia glutinosa Steud (Scrophulariaceae) (RR) were effective in enhancing the proliferation fibroblasts in wound healing (Lau et al, 2008(Lau et al, , 2009 from Panax ginseng C. A. Mey (Araliaceae) could act against UVB-induced growth inhibition and nucleolus damage of HaCaT cells (Lau et al, 2008). Other studies also show that sinomenine from Sinomenium acutum (Thunb.)…”

Section: Introductionmentioning

confidence: 99%

Pearl extract enhances the migratory ability of fibroblasts in a wound healing model

Chen

Young

2012

Pharmaceutical Biology

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Comprehensive proteomic analysis of a Chinese 2‐herb formula (Astragali Radix and Rehmanniae Radix) on mature endothelial cells

Tam

Zhang

et al. 2014

Proteomics

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Endothelial cells are crucially involved in wound healing angiogenesis, restoring blood flow to wound tissues. Our previous study demonstrated that the Chinese 2-herb formula (NF3) possesses significant wound healing effect in diabetic foot ulcer rats with promising in vitro proangiogenic effects on human umbilical vein endothelial cells (HUVEC). Here, we present the comparative global proteome analysis of NF3-treated HUVEC in static or scratch conditions, screening the comprehensive molecular targets in governing the proangiogenic response in wound healing. Our results suggest plasminogen activator inhibitor-1, specifically down-regulated in static condition and Annexin A1 and Annexin A2, up-regulated in scratch condition, as principal proteins responsible for the proangiogenesis in wound healing. We also identified a panel of cytoskeleton regulatory proteins in static and scratch condition, mediating the migratory behavior of NF3-treated HUVEC. The key proteins in static state include myosin regulatory light polypeptide 9, SPAST, tropomyosin (TPM)2, and Vimentin while that in scratch state contained prelamin-A/C, TPM1, TPM2, and Vimentin. In addition, NF3 was shown to regulate transcription and translation, cell-cell interaction, and ROS defense in HUVEC. Proliferation and migration assays further confirmed the identified principal proteins plasminogen activator inhibitor-1 and Annexin A2 which are responsible for NF3-induced proangiogenesis of HUVEC in wound healing. This is the first study on the global proteome expression of NF3-treated HUVEC with the identification of the differences at the molecular level, between static and scratch conditions involved in wound healing angiogenesis.

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Pharmacological investigation on the wound healing effects of Radix Rehmanniae in an animal model of diabetic foot ulcer

Cited by 77 publications

References 38 publications

Efficacy of Some Antibiotics in Curing Resistant <i>Escherichia coli</i> Infection

Efficacy of Some Antibiotics in Curing Resistant <i>Escherichia coli</i> Infection

Pearl extract enhances the migratory ability of fibroblasts in a wound healing model

Comprehensive proteomic analysis of a Chinese 2‐herb formula (Astragali Radix and Rehmanniae Radix) on mature endothelial cells

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Pharmacological investigation on the wound healing effects of Radix Rehmanniae in an animal model of diabetic foot ulcer

Cited by 77 publications

References 38 publications

Efficacy of Some Antibiotics in Curing Resistant &lt;i&gt;Escherichia coli&lt;/i&gt; Infection

Efficacy of Some Antibiotics in Curing Resistant &lt;i&gt;Escherichia coli&lt;/i&gt; Infection

Pearl extract enhances the migratory ability of fibroblasts in a wound healing model

Comprehensive proteomic analysis of a Chinese 2‐herb formula (Astragali Radix and Rehmanniae Radix) on mature endothelial cells

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Product

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Efficacy of Some Antibiotics in Curing Resistant <i>Escherichia coli</i> Infection

Efficacy of Some Antibiotics in Curing Resistant <i>Escherichia coli</i> Infection